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<title>Sensing – Background – TUDA iGEM 2021</title>
<link rel="canonical" href="./sensing-background" />
53 KB (7,122 words) - 16:29, 20 October 2021
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...ost_type menu-item-object-page menu-item-159"><a href="./sensing">Pathogen Sensing</a></li>
...tadt-blocks-nav-heading"><h2 class="section" id="pathogensensing">Pathogen sensing</h2></div>
64 KB (8,992 words) - 19:12, 20 October 2021
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....This construct is derived from...the inducible promoter of the lux quorum sensing system of Vibrio fischeri ...inducible promoter see...']"},{"machine":"BBa_
1.16 MB (178,107 words) - 18:53, 7 August 2021
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<title>Sensing – Circuit – TUDA iGEM 2021</title>
<link rel="canonical" href="./sensing-circuit" />
44 KB (5,953 words) - 16:29, 20 October 2021
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...ost_type menu-item-object-page menu-item-159"><a href="./
sensing">Pathogen
Sensing</a></li>
...mbled our <strong><a href="#pathogensensing" class="ek-link">pathogen
sensing</a> circuit</strong> using GoldenBraid 2.0 cloning. <strong>Functionality o
54 KB (7,522 words) - 16:29, 20 October 2021
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...i>. Combined with the observed high AHL sensitivity, we showed that quorum sensing might be a good mechanism to base this safety circuit on.
<h3>Quorum sensing</h3>
64 KB (8,444 words) - 16:44, 2 December 2021
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...intestine [1,2]. To proof IBDetection, we characterized the tetrathionate sensing TtrS/R system, reproduced the ultrasound-based ARG1 reporter system, and tr
...<h2 class="text-start fw-bolder tab" style="color: #680505">TtrS/R sensing system</h2>
36 KB (4,623 words) - 14:48, 21 October 2021
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...ost_type menu-item-object-page menu-item-159"><a href="./sensing">Pathogen Sensing</a></li>
...our kill-switch.</span></strong> The kill-switch is based on a ComX quorum sensing system. 1) If the cell stays in the biofilm, the ComX concentration is high
99 KB (13,795 words) - 19:10, 20 October 2021
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...<a class="nav-link" href="#Subtitle2">Oxidative Stress
Sensing</a>
...<a class="nav-link" href="#Subtitle3">Interferon-gamma
Sensing</a>
79 KB (9,984 words) - 21:07, 21 October 2021
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...tural chemotaxis pathway to AI-2 of our ECN. AI-2 is the only known quorum sensing signal secreted by H. pylori and also a chemoattractant signal for E. coli.
This ability also plays a role in the quorum sensing (QS), a cell to cell communication process within the population of bacteri
105 KB (13,494 words) - 20:06, 21 October 2021
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...ost_type menu-item-object-page menu-item-159"><a href="./sensing">Pathogen Sensing</a></li>
...trong> that triggers bacteriophage release upon induction through a quorum sensing (QS) signal. Knowledge of the relationship between the number of QS signali
65 KB (8,850 words) - 16:38, 20 October 2021
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...ost_type menu-item-object-page menu-item-159"><a href="./sensing">Pathogen Sensing</a></li>
...n of <strong>metabolic dependency</strong> and the usage of<strong> quorum sensing </strong>or<strong> surface proteins.</strong> Furthermore, to ensure that
191 KB (25,186 words) - 16:41, 20 October 2021
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...<li><a id="run2" class="scrollID" onclick="scrollToID('run2Section')">Sensing</a></li>
...</b>. Staphylococcus aureus itself is a gram-positive bacteria. Its quorum-sensing unit will be more easily expressed on another gram-positive bacteria than a
55 KB (7,093 words) - 15:56, 17 December 2021
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<li class="mark" id="mark2">The Scheme of Quorum Sensing Model</li>
<li class="mark" id="mark4">Simulation of Quorum Sensing Model</li>
28 KB (2,977 words) - 12:17, 14 September 2021
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...ensing"><span class="toc-number">3.2.</span> <span class="toc-text">Oxygen
sensing</span></a></li><li class="toc-item toc-level-3"><a class="toc-link" href="#
...en
sensing</h5><p>The goal of this function is to monitor wound healing by
sensing changes in the oxygen concentration in the wound.<br/>Generally, it is not
42 KB (5,793 words) - 23:16, 15 December 2021
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<a class="nav-link" href="#Subtitle3">Sensing for Stress Biomarkers</a>
...ve oxygen species (ROS) and IFN-γ through the oxidative stress and IFN-γ sensing systems and produce the taurine production enzymes. Finally, with math-base
28 KB (3,467 words) - 07:18, 8 December 2021
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<a id="zone3-2">Quorum sensing</a>
...e, multivirus detection would be realized. Moreover, with LuxI/LuxR quorum sensing system from <span style="font-style: italic;">E. coli</span> used to improv
18 KB (2,271 words) - 03:11, 22 October 2021
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...a> page. The results for each of the three major characterizations (TtrS/R sensing system, ARG1 reporter system, and the entire sensor) will be discussed in t
...arious experiments, resulting in a full dose-response curve for the TtrS/R sensing system, which detects Ttr and produces sfGFP as output (n=1). Besides, for
31 KB (3,916 words) - 15:09, 21 October 2021
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...e="Abstract"></a>Abstract</h3><p>We built an ODE-based model of the Oxygen
Sensing system to simulate the system’s response to oxygen: some genes are expres
...Aim" title="Aim"></a>Aim</h3><p>This model aims to confirm that our Oxygen
Sensing system will respond to oxygen. Although we can predict that the system can
563 KB (66,273 words) - 06:27, 11 December 2021
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  To incorporate quorum sensing, dental pathogen inhibition, and dental restoration into <i>E. coli</i>, we
</svg>Figure 1. Biobrick design overview. (A)Quorum sensing sequence (B) Inhibition sequence (C) Restoration sequence</div>
39 KB (4,562 words) - 11:37, 12 December 2021
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...ed that Hidro can be implemented in diverse scenarios, such as heavy metal sensing, food-quality detection, drug secretion, etc.</p>
...vironment that can be infused with nutrients, allowing for cell growth and sensing, while also providing protection against environmental hazards.</p>
57 KB (7,240 words) - 08:18, 23 November 2021
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...itch. The cell density can be ‘sensed’ by using quorum
sensing. Quorum
sensing is the ability of microorganisms to
...exchanging signaling molecules [24]. In <i>Vibrio fischeri</i> the quorum-
sensing molecule (AHL) is produced by the LuxI (<a href="http://parts.igem.org/Part
93 KB (12,813 words) - 14:27, 2 December 2021
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<a id="zone3-6">2021 Quorum sensing seminar</a>
<strong class="smallTitle">2021 Quorum sensing seminar</strong>
24 KB (2,994 words) - 17:46, 21 October 2021
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<p id="re_8">[8]Shong, J.; Collins, C. H., Quorum sensing-modulated AND-gate promoters control gene
sensing molecule (CAI-1), we engineered the metabolic pathways of <i>E. coli</i> to
46 KB (5,069 words) - 03:59, 22 October 2021
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<title>Pathogen Sensing – TUDA iGEM 2021</title>
<link rel="canonical" href="./sensing" />
27 KB (3,318 words) - 16:29, 20 October 2021
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...d to be heavily used in future patient care. Specifically, electrochemical sensing technologies are getting attention due to their label-free capability and r
...e channel for placement of the electrodes and to allow for electrochemical sensing (middle) and a final reservoir with rounded and interlocked hexagon micropi
168 KB (22,723 words) - 02:02, 17 December 2021
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...aderlink" href="#Goal" title="Goal"></a>Goal</h4><p>To construct an oxygen sensing system using oxygen-responsive promoters pAnb1 and pRox1 from <em>Saccharom
.../em> BY4741 strain, we constructed the yeast strains which have the oxygen sensing system.</p>
30 KB (4,302 words) - 06:29, 11 December 2021
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...ing Theme Meet Up, we found that both of our teams were involved in quorum sensing design, so we exchanged the methods of and precautions for co-cultivation o
18 KB (2,869 words) - 02:45, 25 September 2021
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...ost_type menu-item-object-page menu-item-159"><a href="./sensing">Pathogen Sensing</a></li>
...ng> secreted by the pathogens for intercellular communication. The initial sensing triggers a defense reaction cascade within the sleeper cells. This cascade
58 KB (7,417 words) - 16:28, 20 October 2021
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<a id="zone3-5">Model 2 Quorum Sensing System</a>
...gn" class="href1">design</a> of our project is based on AHL-induced quorum sensing (QS) system to amplify the detection effect of engineered bacteria on virus
33 KB (4,658 words) - 03:30, 22 October 2021
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...ing Theme Meet Up, we found that both of our teams were involved in quorum sensing design, so we exchanged the methods of and precautions for co-cultivation o
18 KB (2,868 words) - 08:57, 2 October 2021
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<a id="zone3-6">2021 Quorum sensing seminar</a>
<strong class="smallTitle">2021 Quorum sensing seminar</strong>
24 KB (3,020 words) - 15:37, 14 October 2021
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<a id="zone3-5">Model 2 Quorum Sensing System</a>
...gn" class="href1">design</a> of our project is based on AHL-induced quorum sensing system to amplify the detection effect of engineered bacteria on viruses. T
33 KB (4,661 words) - 06:55, 16 October 2021
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...,null),"[8] Yang Fang, Zhangle, et al. Progress in the study of population sensing system in Pseudomonas aeruginosa. China Journal of Antibiotics. 2012, 37(1)
17 KB (2,633 words) - 08:32, 21 October 2021
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...,null),"[8] Yang Fang, Zhangle, et al. Progress in the study of population sensing system in Pseudomonas aeruginosa. China Journal of Antibiotics. 2012, 37(1)
17 KB (2,632 words) - 03:36, 21 October 2021
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...,null),"[8] Yang Fang, Zhangle, et al. Progress in the study of population sensing system in Pseudomonas aeruginosa. China Journal of Antibiotics. 2012, 37(1)
17 KB (2,632 words) - 03:39, 21 October 2021
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...,null),"[8] Yang Fang, Zhangle, et al. Progress in the study of population sensing system in Pseudomonas aeruginosa. China Journal of Antibiotics. 2012, 37(1)
17 KB (2,631 words) - 04:01, 21 October 2021
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...,null),"[8] Yang Fang, Zhangle, et al. Progress in the study of population sensing system in Pseudomonas aeruginosa. China Journal of Antibiotics. 2012, 37(1)
17 KB (2,667 words) - 16:47, 21 October 2021
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...ailable research, our project’s objective is to design a specific quorum sensing system that “rapidly” detects naturally occurring bacteria in food/beve
<h2>Quorum Sensing With Autoinducer II</h2>
6 KB (909 words) - 02:05, 22 October 2021
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...Y., Ajmal, M., Chen, J. L., & Yu, D. (2017). Molecular Mechanism of Quorum-Sensing in Enterococcus faecalis: Its Role in Virulence and Therapeutic Approaches.
21 KB (3,215 words) - 02:44, 25 September 2021
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...ight be extremely low in practical application. Thus, we focused on quorum sensing.<br>
We selected LuxI/LuxR quorum sensing system in <span style="font-style: italic;">E. coli</span>.<br><br>
23 KB (2,720 words) - 03:20, 22 October 2021
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<a href="#Quorum
sensing"><span>Quorum
sensing</span></a></li>
<div class="subtitle" id="Quorum
sensing">
13 KB (1,668 words) - 04:01, 22 October 2021
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...Y., Ajmal, M., Chen, J. L., & Yu, D. (2017). Molecular Mechanism of Quorum-Sensing in Enterococcus faecalis: Its Role in Virulence and Therapeutic Approaches.
21 KB (3,215 words) - 08:56, 2 October 2021
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...Y., Ajmal, M., Chen, J. L., & Yu, D. (2017). Molecular Mechanism of Quorum-Sensing in Enterococcus faecalis: Its Role in Virulence and Therapeutic Approaches.
21 KB (3,215 words) - 11:41, 8 October 2021
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2021 iGEM Quorum Sensing Science Symposium
...rated with BUCT, Jilin_China, and NEU_CHINA by organizing 2021 iGEM Quorum-sensing Science Symposium.
21 KB (2,447 words) - 12:46, 21 October 2021
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...Y., Ajmal, M., Chen, J. L., & Yu, D. (2017). Molecular Mechanism of Quorum-Sensing in Enterococcus faecalis: Its Role in Virulence and Therapeutic Approaches.
21 KB (3,213 words) - 09:47, 16 October 2021
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...Y., Ajmal, M., Chen, J. L., & Yu, D. (2017). Molecular Mechanism of Quorum-Sensing in Enterococcus faecalis: Its Role in Virulence and Therapeutic Approaches.
21 KB (3,214 words) - 14:11, 17 October 2021
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...Y., Ajmal, M., Chen, J. L., & Yu, D. (2017). Molecular Mechanism of Quorum-Sensing in Enterococcus faecalis: Its Role in Virulence and Therapeutic Approaches.
21 KB (3,213 words) - 04:35, 18 October 2021
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...solve this problem, we improved the current biobrick by adding the quorum sensing system.
<strong class="smallTitle">Quorum Sensing System</strong>
10 KB (1,190 words) - 16:52, 21 October 2021
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<a class="nav-link" href="#Subtitle2">
Sensing Systems</a>
<p>  We engineered <i>E. coli</i> Nissle 1917 to have two
sensing systems, which are induced by stress biomarkers interferon-gamma (IFN-γ) a
24 KB (3,559 words) - 00:03, 5 December 2021
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...The <i>p</i>-Cresol sensing bacteria can be replaced with other biomarker sensing bacteria, thus enabling <b>CreSense</b> to have a wide range of application
...Cresol sensing bacteria. In order to allow survival of the <i>p</i>-Cresol sensing bacteria inside the blood and produce fluorescent protein, it is necessary
43 KB (4,256 words) - 07:45, 4 July 2021
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...ost_type menu-item-object-page menu-item-159"><a href="./
sensing">Pathogen
Sensing</a></li>
...s="ek-link">Biosafety</a> and <a href="./
sensing" class="ek-link">Pathogen
Sensing</a>) </p>
23 KB (3,013 words) - 19:09, 20 October 2021
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...cteria. In addition, to enhance the sensitivity of virus detection, quorum sensing system was applied to transfer the signal to the whole population of bacter
<strong class="smallTitle">Quorum Sensing</strong>
16 KB (2,085 words) - 02:51, 22 October 2021
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...ost_type menu-item-object-page menu-item-159"><a href="./sensing">Pathogen Sensing</a></li>
...x">The water pathogen <em>Pseudomonas</em> <em>aeruginosa</em> uses quorum sensing molecules. </p>
25 KB (3,138 words) - 14:28, 20 October 2021
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...echnique of Staphylococcus aureus itself into our chassis organism. Quorum
sensing is a technique where environment-dependent gene regulation happens by cell-
<p>To know how we have incorporated this quorum
sensing network in our chassis organism, refer to our <a class="referenceLinks" hre
29 KB (3,815 words) - 01:28, 22 October 2021
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<li class="mark" id="mark6">Quorum Sensing System</li>
<h1 class="topic" id="topic6">Quorum Sensing System</h1>
51 KB (5,380 words) - 03:48, 22 October 2021
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...d on this evidence, we can believe that E. coli can express the Fsr quorum sensing system of Enterococcus faecalis.")]})))),i.a.createElement(c.a,{headerText:
73 KB (11,710 words) - 02:45, 25 September 2021
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...>In this project, we tested the performance of Chromoproteins, mercury ion sensing circuit, and L-dopa production pathways in hydrogels, and documented the de
<h3 style="padding-left:2vw;">Mercury ion sensing</h3>
21 KB (2,356 words) - 14:29, 21 October 2021
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1. 2021 iGEM Quorum Sensing Science Symposium
...lated to quorum sensing. Our team have then organized the 2021 iGEM Quorum-sensing Symposium with BUCT, Jilin_China, and NEU_CHINA. The symposium information
23 KB (2,684 words) - 14:40, 19 October 2021
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<a id="zone3-2">Quorum sensing system</a>
<strong class="smallTitle">Quorum sensing system</strong>
16 KB (1,938 words) - 03:28, 22 October 2021
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...up, we learned that the BS_United _China's project applied the same quorum sensing system as our project. Therefore, NEU_China establish cooperative partnersh
...ng AHL production and various factors. Our team's approach of using quorum sensing system to amplify weak signals also provided a new idea for BS_United_China
11 KB (1,267 words) - 16:53, 21 October 2021
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...d on this evidence, we can believe that E. coli can express the Fsr quorum sensing system of Enterococcus faecalis.")]})))),i.a.createElement(c.a,{headerText:
73 KB (11,710 words) - 08:57, 2 October 2021
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...d on this evidence, we can believe that E. coli can express the Fsr quorum sensing system of Enterococcus faecalis.")]})))),i.a.createElement(c.a,{headerText:
73 KB (11,710 words) - 11:42, 8 October 2021
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<title>Sensing – Laboratory – TUDA iGEM 2021</title>
<link rel="canonical" href="./sensing-laboratory" />
29 KB (3,780 words) - 16:29, 20 October 2021
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..., lactobacillus would constitutively reduce AHL, which is a kind of quorum sensing molecule
additional trigger – quorum sensing molecules of the specific pathogens. For instance, here we
7 KB (746 words) - 20:35, 21 October 2021
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1. 2021 iGEM Quorum Sensing Science Symposium
...lated to quorum sensing. Our team have then organized the 2021 iGEM Quorum-sensing Symposium with BUCT, Jilin_China, and NEU_CHINA. The symposium information
23 KB (2,684 words) - 13:05, 20 October 2021
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...<i>P. putida</i>, we designed a cell density-dependent kill switch. Quorum sensing is a mechanism can be used to ‘sense’ the cell density [22], and will b
...itoxin system again. This mechanism relies on the production of the quorum sensing molecule AHL in the cell, which rapidly diffuses in and out of any cell and
81 KB (11,133 words) - 11:56, 2 December 2021
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...ity, more specifically: thiosulfate and tetrathionate. More details on the
sensing part can be read on our <a class="pagelink" target="blank" href="https://20
...our user case, we postulated that the integration of this highly specific
sensing part with the novel ARG would indeed result in a sensor design that is not
37 KB (4,676 words) - 12:30, 21 October 2021
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...nk" href="https://2021.igem.org/Team:TU-Eindhoven/Proof_Of_Concept">TtrS/R sensing system</a>. To have more control over the expression of the TtrR and TtrS p
...econd one succeeded, giving us altered bacteria which should behave as the sensing part. To test this, we prepared all of the remedies as well as any addition
36 KB (4,415 words) - 15:37, 21 October 2021
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...ass="hugo-figure-caption">Fig. 7. Schematic representation of the cgCRISPR sensing device.</p>
level of activation, the longer sensing loop could cause unintended activation,
34 KB (4,166 words) - 12:50, 1 December 2021
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<h1 class="topic" id="topic4">Quorum Sensing System</h1>
...different amounts of bacteria in dogs’ oral cavities by using the Quorum Sensing system(QS system).
34 KB (3,466 words) - 11:01, 18 September 2021
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...ost_type menu-item-object-page menu-item-159"><a href="./
sensing">Pathogen
Sensing</a></li>
...tle ub-content-toggle-title-b2909100-40a0-487a-b042-4b182bff21e7">Pathogen
Sensing </p><div class="wp-block-ub-content-toggle-accordion-toggle-wrap right">
87 KB (12,240 words) - 16:28, 20 October 2021
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...ost_type menu-item-object-page menu-item-159"><a href="./sensing">Pathogen Sensing</a></li>
..."ek-link">BBa_K3985003</a></td><td>Coding Sequence </td><td>QscR, a quorum sensing transcripion factor which binds 3OC<sub>12</sub>-HSL, codon-optimized for <
30 KB (4,211 words) - 16:29, 20 October 2021
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...to plants. For this purpose we came up with an approach to use the quorum sensing system of the rhizosphere inhabiting Bacteria.
This, then, should lead to the excretion of AHLs as quorum sensing molecules. The cell containing BBa_3928003 thereby works as a sender cell t
23 KB (2,968 words) - 10:34, 21 October 2021
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...BS_United_China and DUT-China on plasmid construction, AHL-mediated quorum sensing system and model respectively. We also worked with LZU-China and NMU_China
...AHL threshold, so our two teams had certain complementary roles in quorum sensing. Then our cooperation carried out.<br><br>
14 KB (1,726 words) - 15:56, 21 October 2021
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...<span class="figure">Figure 2. PttB344_HrpS_PJ23105_ttrR,
sensing S4O62- to release the substance S</span>
...<li><p>Part1: <b style="color: #44546A">PyeaR_HrpR</b>,
sensing NO<sup>3-</sup> to release the substance
15 KB (1,569 words) - 14:04, 19 October 2021
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...Escherichia coli* To Use Endogenous H2S as a Signaling Molecule for Quorum Sensing. *ACS synthetic biology*, *8*(9), 2113–2120. \n\n[2] https://2010.igem.or
337 KB (51,101 words) - 03:58, 22 October 2021
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...="text-end fw-bolder" style="color: #56cccc; font-size:100%">Article - IBD sensing bacteria [2]</h2>
...the sensor mechanism of tetrathionate is further optimized. To report the sensing of these molecules, a cascade is activated which will result in the express
112 KB (14,019 words) - 14:13, 21 October 2021
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...uence placed after it instead of inhibiting it. Therefore, we modified our sensing design to adapt it to their project :</p>
...in precise quantities to assess the safety of the water. By modifying our sensing part, we would be able to detect them and trigger a response by Shewanella.
28 KB (4,263 words) - 02:02, 22 October 2021
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<h3>Mercury sensing</h3>
...mercury ion (10μM) at the ratio of 1:100. Use the same amount of mercury-sensing bacteria and M9 medium plus antibiotics for serial dilution. </p>
38 KB (4,750 words) - 14:45, 9 December 2021
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...ost_type menu-item-object-page menu-item-159"><a href="./sensing">Pathogen Sensing</a></li>
...oof of concept experiments and started the planing of our <strong>pathogen sensing genetic circuit</strong>.</p>
103 KB (13,229 words) - 16:29, 20 October 2021
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...ent of fish corruption detection, mainly targeting the detection of quorum sensing signal molecules of Pseudomonas aeruginosa. In the project internal communi
...life activities of Pseudomonas aeruginosa are inseparable from its quorum sensing system, and the existence of QS signal molecules is the reason for its rapi
19 KB (2,824 words) - 02:42, 22 October 2021
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..."br",null),"[2].Yong YC, Wu XY, Sun JZ, Cao YX, Song H. Engineering quorum sensing signaling of Pseudomonas for enhanced wastewater treatment and electricity
17 KB (2,792 words) - 16:47, 21 October 2021
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<th>The thiosulfate sensing system under the control of J23103</th>
<th>The thiosulfate sensing system under the control of J23104</th>
38 KB (2,978 words) - 01:46, 22 October 2021
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...pH response switch inhibited the expression of genes related to the quorum sensing system at a lower pH.","tap":{},"color":{},"link":[],"st":["text-align-j"]}
12 KB (1,426 words) - 02:04, 22 October 2021
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Fatty Acid Sensing Module
Acid Sensing Module, Fatty Acid Consumption Module and Safety Module. With four aspects
30 KB (3,165 words) - 02:21, 22 October 2021
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..."br",null),"[2].Yong YC, Wu XY, Sun JZ, Cao YX, Song H. Engineering quorum sensing signaling of Pseudomonas for enhanced wastewater treatment and electricity
17 KB (2,764 words) - 03:36, 21 October 2021
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..."br",null),"[2].Yong YC, Wu XY, Sun JZ, Cao YX, Song H. Engineering quorum sensing signaling of Pseudomonas for enhanced wastewater treatment and electricity
17 KB (2,764 words) - 03:39, 21 October 2021
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  With quorum sensing and repressible promoter, our biobrick has a time span to achieve oral inhi
...eed 37 degrees to enable its mRNA to begin translation and initiate quorum sensing regulation. In this way, we can ensure that our engineered bacteria can ope
11 KB (1,084 words) - 03:12, 22 October 2021
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...of the chitin oligosaccharide N-acetylglucosamine by expressing the chitin-sensing one-component system from <i> Vibrio cholerae [2,3] </i> in <i> E. coli </i
...nce we figured out which biomarker we wanted to use, we began looking into sensing pathways to detect this oligosaccharide. </p>
20 KB (2,682 words) - 20:47, 18 December 2021
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...s guards who are transformed from workers through the activation of quorum-sensing (QS) system. After the transformation, guards secrete a toxin to selectivel
...eaters that evolve from workers. They interact with each other through two sensing systems, Pmr and Agr.
16 KB (2,004 words) - 02:38, 22 October 2021
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...organism. Thus, we implemented <i>luxI</i> and <i>luxR</i> to allow quorum sensing to take place and provide a means of communication between bacterias in res
...rong>biodegradation, and lysis</strong>, which will be regulated by quorum sensing. This device begins with the stress sensitive promoter algD, which will ini
16 KB (2,292 words) - 21:52, 21 October 2021
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...><a class="navmenu scroll" href="#mainblock3" data-menuanchor="firstPage">>Sensing</a></li>
<h2>Sensing</h2>
26 KB (3,288 words) - 23:18, 21 October 2021
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...of dsRNA molecules via a cell lysis protein, that is only activated Quorum sensing mechanism triggers cell lysis</p>
...grow exponentially, AHL molecules act as communication signalS for quorum sensing. <b>C.</b> Once the concentration of lysis protein reach a threshold, cells
46 KB (5,851 words) - 20:46, 19 December 2021
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...life activities of Pseudomonas aeruginosa are inseparable from its quorum sensing system, and the existence of QS signal molecules is the reason to let it ra
...sign and experimental methods. The adviser of NDNF once studied the quorum sensing system of Pseudomonas aeruginosa and can help GCGS with the QS signal syste
22 KB (2,569 words) - 15:09, 21 October 2021
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...e, the ovastacin could be regulated with the help of some specific hormone
sensing systems.</p>
...<p>As per the planning in design, progesterone and estrogen
sensing systems in bacteria were looked into. One of them was the mifepristone (pro
104 KB (12,654 words) - 03:14, 17 December 2021
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...The "Detection & Report" module can detect the occurrence of IBD by sensing the signal of
...e. The "Suicide" module mainly adopts the strategy of realizing suicide by sensing the
44 KB (4,004 words) - 02:07, 18 November 2021
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<h2>Growth phase association: quorum sensing </h2>
...its implications are much more extensive, since “quorum sensing allows groups of
226 KB (19,581 words) - 02:32, 18 December 2021
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...BBa_K3883036. The new Part have a stronger inhibitory effect on the quorum sensing system controlled by promoter Ptrc. Our team members agreed that this new c
18 KB (2,327 words) - 12:23, 21 October 2021
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...r institute to discuss the idea, looked into lex A system for progesterone sensing, completely ruled out production of NAG enzyme by bacteria </p>
...use as chassis by looking into secretion systems, looked into progesterone sensing by the SRTF1 . </p>
67 KB (6,526 words) - 03:24, 17 December 2021
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...homogeneous-phase fluorescence anisotropy sensing of tau Proteins. Anal
...homogeneous-phase fluorescence anisotropy sensing of tau Proteins. Anal
103 KB (6,956 words) - 14:50, 21 October 2021
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...ost_type menu-item-object-page menu-item-159"><a href="./sensing">Pathogen Sensing</a></li>
...a href="#ourcircuit" class="ek-link">phage induction</a> through a quorum sensing signal and the release of a phage from a new host organism. The whole conce
84 KB (12,113 words) - 16:29, 20 October 2021
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...ur part collection gives a detailed overview of the complete tetrathionate sensing two-component system TtrS/R, followed by the formation of ultrasound measur
<td class="tg-rb3d">TtrS is the first component of the two-component sensing system TtrS/R and functions as the membrane-bound sensor kinase (SK), which
18 KB (2,360 words) - 19:25, 20 October 2021
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<h3><b>2. EsaR-based quorum-sensing system</b></h3>
...n</a> page). We hope they can help future teams to construct better quorum-sensing systems.
15 KB (1,758 words) - 19:22, 21 October 2021
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<li>Yellow = sensing loop (important for RNA recognition)</li>
<li>Blue = extended sensing domain</li>
161 KB (22,755 words) - 12:50, 1 December 2021
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<td>Sensing system for Vitamin B12 inducing the production of lactate</td>
<td>Optimised sensing system for Vitamin B12 inducing the production of lactate</td>
22 KB (2,804 words) - 01:33, 22 October 2021
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...and luxR genes. These genes will enable the chassis to partake in a quorum sensing system with the second device, which will enable them to act in accordance
...different concentrations. By using this promoter, we look to create a bio sensing system and be able to regulate future biodegradation.
17 KB (2,514 words) - 03:23, 22 October 2021
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...ods that can inspire and benefit other iGEM teams. In our oxidative stress sensing system experiments, we collected data that prove paraquat as a more suitabl
...rts.igem.org/Part:BBa_K2610031"> BBa_K2610031 </a> in the oxidative stress sensing system for the following reasons: </p>
26 KB (3,031 words) - 08:11, 5 December 2021
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...S/R for
sensing thiosulfate, ttrS/R for
sensing tetrathionate and NorR for
sensing nitric oxide.<sup>[28]</sup><br/>
<p class="figname">Fig.16 Principle for thrS/R and ttsS/R
sensing system. Figure reference from BBa_K2507004 and BBa_2507006</p><br/>
32 KB (4,722 words) - 03:23, 22 October 2021
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<br>2. The quorum sensing system<br>
...among Xanthomonas species. Therefore, we conducted research on the quorum sensing system in the targeted bacterial strains and the conclusions are as follows
28 KB (4,031 words) - 03:45, 22 October 2021
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...i</em> codon-optimized basic parts TtrS and TtrR are both derived from the sensing two-component system of the marine bacterium <em>Shewanella Baltica</em>. T
...tps://2021.igem.org/Team:TU-Eindhoven/Results">Results </a> segment TtrS/R sensing system. An overview of all components of the TtrS/R plasmids can be seen in
18 KB (2,121 words) - 14:17, 21 October 2021
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...represent the entire process of the detection officer from sensing the taste
after sensing the taste-presenting substances.
161 KB (11,046 words) - 17:17, 17 November 2021
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...macrophage that can automatically adjust its state of function in vivo by sensing the changes within the microenvironment.
<b>Sensing IL-6:</b> With the IL-6 sensor on the surface of Toggle Macrophage, it can
28 KB (4,014 words) - 02:21, 22 October 2021
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...href="#references">16</a>], a small signaling molecule involved in quorum sensing (QS) activities. Since QS is used by many bacterial species to coordinate p
...ize an already existing 3OC6HSL receiver (BBa_T9002) to test our phosphate sensing device. Future challenges will imply to investigate its actual ability to s
27 KB (3,468 words) - 12:41, 20 October 2021
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Bacterial quorum-sensing molecules, like N-acyl homoserine lactones (AHL), are one of the most preva
...relationship between the phosphorus in the soil that activates the quorum sensing signalling through to the effect it will have in the rhizobial cell (we cal
32 KB (4,298 words) - 10:44, 21 October 2021
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Fatty acid sensing module
acid-sensing system (BBa_K3763001, BBa_K3763002, BBa_K3763003) was constructed to enable
21 KB (2,181 words) - 02:29, 22 October 2021
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...:{},"link":[],"st":["text-align-l"]}]},{"pos":"t2","word":[{"char":"Quorum sensing","tap":{},"color":{},"link":[],"st":["text-align-l"]}]},{"pos":"maintext2",
26 KB (3,268 words) - 11:41, 21 October 2021
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...ong Kong in 2012 as the degrading enzyme of AHL (the most important quorum sensing molecule of Gram-negative bacteria). But in the part we designed this year,
<i class="imgTitle">Fig.5 The mCherry is expressed when <i>E. bsuahlscout</i> sensing the AHL in the environment.</i>
25 KB (2,988 words) - 12:28, 21 October 2021
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<li>Yellow = sensing loop (important for RNA recognition)</li>
<li>Blue = extended sensing domain</li>
149 KB (20,818 words) - 17:50, 20 October 2021
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...macrophage that can automatically adjust its state of function in vivo by sensing the changes within the microenvironment.
<b>Sensing IL-6:</b> With the IL-6 sensor on the surface of Toggle Macrophage, it can
26 KB (3,798 words) - 07:50, 11 October 2021
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..., Jingping Hu, et. al. Enhanced quorum sensing of anode biofilm for better sensing linearity and recovery capability of microbial fuel cell toxicity sensor. E
14 KB (2,259 words) - 11:42, 18 October 2021
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...<li><p>Part1: <b style="color: #44546A">PyeaR_HrpR</b>,
sensing NO<sup>3-</sup> to release the substance
...<li><p>Part2: <b style="color: #4472C4">PttB344_HrpS_PJ23105_ttrR</b>,
sensing
16 KB (1,815 words) - 14:07, 19 October 2021
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...<li><p>Part1: <b style="color: #44546A">PyeaR_HrpR</b>,
sensing NO<sup>3-</sup> to release the substance
...<li><p>Part2: <b style="color: #4472C4">PttB344_HrpS_PJ23105_ttrR</b>,
sensing
19 KB (2,140 words) - 14:07, 19 October 2021
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...our project, cyclic voltammetry was essential for characterizing modified sensing devices. It is conducted using a potentiostat, which was not accessible for
...ure the potentiostat and we conducted cyclic voltammetry to characterize a sensing device.
74 KB (7,830 words) - 01:44, 17 December 2021
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..., Jingping Hu, et. al. Enhanced quorum sensing of anode biofilm for better sensing linearity and recovery capability of microbial fuel cell toxicity sensor. E
14 KB (2,259 words) - 03:36, 21 October 2021
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acids-sensing system was constructed to enable precise therapeutics actuation only in app
Fatty Acid Sensing Module, Fatty Acid Consumption Module and Safety Module.</p>
32 KB (3,403 words) - 03:28, 22 October 2021
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..., Jingping Hu, et. al. Enhanced quorum sensing of anode biofilm for better sensing linearity and recovery capability of microbial fuel cell toxicity sensor. E
15 KB (2,288 words) - 23:18, 21 October 2021
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...extracellular molecule, eliciting population-wide behaviors such as quorum sensing, cell differentiation, and averaging. Drawing inspiration from collective b
...e will express this dilemma by using a genetic circuit centering in quorum sensing. We will also provide various strategies and aim to determine the best stra
9 KB (1,334 words) - 19:56, 15 March 2021
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...which includes the various parts required for our system. We combined the
sensing two-component system TtrS/R (<a class="pagelink" target=" blank" href= "ht
...we also prove that ARG1 as reporter system can be combined with different
sensing systems, e.g. thiosulfate sensor, meaning it is possible to develop multipl
10 KB (1,218 words) - 13:58, 21 October 2021
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...modeled to lead to the transcription and translation of TtrS, which is the sensing protein, and TtrR, the regulator protein. In reality, TtrS and TtrR are pre
...ure 1 step 4-7). The cascade is modeled as binding of tetrathionate to the sensing protein TtrS, inducing autophosphorylation of TtrS (Figure 1 step 4). TtrR
34 KB (4,188 words) - 14:35, 21 October 2021
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...n (CBD). Our system secretes a biofilm degrading enzyme, Dispersin B upon sensing the AI-2 autoinducer commonly present during bacterial colonization. Additi
<li class="model-li">The NaCMC/HPMC mix is chosen as the self-sensing smart material for the catheter application. Since the carboxymethyl (CMC)
18 KB (2,559 words) - 02:06, 22 October 2021
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of pathogenic bacteria, through quorum sensing signals and respond by secreting
(AI-2)/Lsp - Operon quorum sensing mechanism. (Bentley and Hooshangi, 2011.) We
13 KB (1,543 words) - 02:11, 22 October 2021
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<h1>Phase 1 : Vitamin B12 sensing</h1>
...GEM wiki pages, we identified the Cobalamin riboswitch as the basis of our sensing module. This riboswitch relies on the sequestration of the RBS located down
15 KB (2,118 words) - 01:45, 22 October 2021
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<h3><b><i>Characterization of a Quorum Sensing regulator protein EsaR and its variants</i></b></h3>
...the repression of TolB expression when engineered bacteria perceive quorum sensing molecules from pathogens. This process is mediated by the EsaR regulator an
31 KB (4,461 words) - 08:12, 14 December 2021
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.../i> zoosporangia, tryptophol, which is secreted by <i> Bd </i> as a quorum sensing metabolite, and the amphibian’s T4 hormone that acts as a chemoattractant
...sensor kinase to detect chitin. Differing from prototypical two-component sensing systems, the ChiS inter-membrane protein both recognizes chitin and directl
24 KB (3,455 words) - 07:25, 18 December 2021
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...Our first contribution was that we found a new way to recognize the quorum sensing (QS) molecule. aptamer was a single strand of DNA, it can conjugate with mo
...a team who designed a biosensor that can detect the corruption of milk by sensing 3O-C6-HSL(https://2019.igem.org/Team:Michigan）. The biosensor concluded a
14 KB (2,068 words) - 02:21, 22 October 2021
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...o additional post translational modification sensors for our central dogma sensing circuit: a protease sensor and a glycosylation sensor.</p>
...offered new ideas of other concepts related to orthogonality for potential sensing systems. He suggested that our team design orthogonality sensors targeting
45 KB (7,150 words) - 21:31, 21 October 2021
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...ainties as the FsrC protein, which is an important component of the quorum-sensing system is a membrane protein, and due to the structural difference of cell
19 KB (3,138 words) - 02:44, 25 September 2021
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...etect the inflammation marker nitric oxide, and a pathogen-specific quorum
sensing factor named autoinducer-3. Upon activation, the genetic circuit expresses
<p style="font-size: 16px; font-family: 'Montserrat', sans-serif;"> Quorum
sensing (QS) is the process used by both gram-negative and gram-positive bacteria t
22 KB (2,987 words) - 19:57, 21 October 2021
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...e performed in the BSL-1 laboratory as per required guidelines. The quorum sensing unit is cloned into E-coli DH5𝛼 and E. coli BL21 and KRX for expression
...cteria aims to detect the presence of the target pathogen using the quorum sensing system and then secrete a bacteriocin. This bacteriocin is more specific to
21 KB (2,562 words) - 15:43, 21 October 2021
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...-6;padding-left:4vw;">Gibson assembly was used in building the mercury ion sensing plasmid and caffeine detection plasmid system that initially used red fluor
...w;">We tested the function of the previously constructed strain capable of sensing mercury ions in the environment and expressing fluorescent proteins, and th
29 KB (3,485 words) - 09:58, 21 October 2021
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...at quickly. To bring the cells to do such things we wanted to use a quorum sensing system with one receiver and a sender cell. <br><br>
...n the soil where the sender cell detects phosphate levels, excretes quorum sensing molecules like HSL and the receiver cell perceives this and expresses a mar
40 KB (5,857 words) - 16:59, 20 October 2021
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...elieve that if our delivery was optimal, there would be no need for quorum sensing.<br/>Our control mechanism at this point was planned to make Riboswitches r
...content: 'After this meeting, we decided to scrap the idea of quorum sensing in favour of a more optimal delivery system. This would reduce the complexi
62 KB (8,480 words) - 20:29, 15 December 2021
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...ost_type menu-item-object-page menu-item-159"><a href="./
sensing">Pathogen
Sensing</a></li>
...href="./
sensing" class="ek-link">Pathogen
Sensing</a>” group created our
sensing system, which enables our biofilm to detect pathogens.</li><li>The “<a hr
21 KB (2,696 words) - 16:29, 20 October 2021
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LuxI/LuxR quorum sensing system was designed to improve the sensitivity of detection. This system al
...nsity of engineered bacteria without quorum sensing system and with quorum sensing.
14 KB (1,724 words) - 03:24, 22 October 2021
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...vity is not enough for induction. Hence we set out to look for alternative sensing molecules.</p>
...as the fertilisation window of the ovum is short. Even if the progesterone sensing molecule is sensitive to a low concentration of progesterone, under any cir
151 KB (15,888 words) - 03:30, 17 December 2021
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...biofilter is higher than outside. To ‘sense’ the cell density, quorum sensing is used, which is the ability of bacteria to regulate gene expression depen
...v class="text"><figcaption><strong>Figure 8:</strong> The quorum sensing compound AHL (blue circles) is produced by LuxI. AHL forms a complex with L
51 KB (6,715 words) - 23:09, 2 December 2021
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...igem.org/Team:Wageningen_UR/Wetlab/QuorumSensing" class="text-link">quorum sensing</a> can be coupled to the methane kill switch. We hypothesised that this wo
...tors and Sensory Sites of Formaldehyde-responsive Regulator FrmR and Metal-sensing Variant * □, 291 (2016) 19502–19516. https://doi.org/10.1074/jbc.M116.7
80 KB (10,607 words) - 21:45, 19 November 2021
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...FrmA and FrmB catabolize this toxic compound [8]. This protein presents a sensing system that can detect levels as low as 1 μM of formaldehyde [9, 10].
...i>K. J. Denby, J. Iwig, C. Bisson, et al., The mechanism of a formaldehyde-sensing transcriptional regulator. Sci. Rep. 6, 1–15 (2016). </li>
59 KB (7,708 words) - 16:39, 2 December 2021
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...horus monitoring solutions. This report will cover the existing phosphorus sensing methods available on the market and explore their advantages and disadvanta
...of phosphorus their farms release into the environment. Of the phosphorus sensing devices that exist there are two primary soil sensors and runoff sensors. S
9 KB (1,443 words) - 23:21, 21 October 2021
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for directed evolution in fatty acids sensing module. In high-throughput arrays, bacteria that have
sensing module</p>
53 KB (4,884 words) - 03:34, 22 October 2021
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Fatty Acid Sensing Module
<h2>Fatty Acid Sensing Module</h2>
31 KB (3,077 words) - 02:30, 22 October 2021
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...+Azurite cassettes</strong> with which we wanted to check the progesterone
sensing. SRTF1 is expressed under a constitutive promoter SP126 <a target="_blank"
...<p>From Phase 2, we will be able to show progesterone
sensing and ovastacin regulation. This would be due to the confirmation of the inte
58 KB (6,366 words) - 03:17, 17 December 2021
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<h3>Progesterone sensing</h3>
...one_sensing_in_SRTF1-P22_Combined_Cassette.png" alt="Graph of Progesterone sensing in SRTF1-P22 Combined Cassette" style="width:100%">
46 KB (5,129 words) - 03:20, 17 December 2021
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...ainties as the FsrC protein, which is an important component of the quorum-sensing system is a membrane protein, and due to the structural difference of cell
19 KB (3,138 words) - 08:56, 2 October 2021
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...ainties as the FsrC protein, which is an important component of the quorum-sensing system is a membrane protein, and due to the structural difference of cell
19 KB (3,138 words) - 11:41, 8 October 2021
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.... “Growth Regulation in Amphibian Pathogenic CHYTRID Fungi by the Quorum Sensing Metabolite Tryptophol.” Frontiers in Microbiology, vol. 9, 2019, doi:10.3
...s one of the inputs that induce violacein production. Below are some light sensing systems we considered during the design process.</p>
23 KB (3,618 words) - 02:04, 22 October 2021
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...<li><p>Part1: <b style="color: #44546A">PyeaR_HrpR</b>,
sensing NO<sup>3-</sup> to release the substance
...<li><p>Part2: <b style="color: #4472C4">PttB344_HrpS_PJ23105_ttrR</b>,
sensing
13 KB (1,471 words) - 14:04, 19 October 2021
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...between OxyR and FimH and get a second opinion on the inclusion of quorum sensing within our project.
With regards to quorum sensing, Dr. Shanks expressed his belief that pursuing FimH would be a better fit f
5 KB (832 words) - 17:11, 21 October 2021
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gene transcription. In addition, the Pi-sensing pathway
sensing (QS). The concentration of QS signaling molecules
38 KB (4,787 words) - 17:35, 21 October 2021
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...ainties as the FsrC protein, which is an important component of the quorum-sensing system is a membrane protein, and due to the structural difference of cell
19 KB (3,138 words) - 09:46, 16 October 2021
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<br>Detect <i>H. pylori</i> through quorum sensing molecules, AI-2,<sup>21,22</sup> and swim toward.<sup>23–25</sup> This ap
<li>Plummer P. LuxS and quorum-sensing in Campylobacter. Front Cell Infect Microbiol. 2012;2:22.</li>
39 KB (3,888 words) - 19:47, 21 October 2021
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...ainties as the FsrC protein, which is an important component of the quorum-sensing system is a membrane protein, and due to the structural difference of cell
19 KB (3,138 words) - 14:11, 17 October 2021
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...<li><p>Part1: <b style="color: #44546A">PyeaR_HrpR</b>,
sensing NO<sup>3-</sup> to release the substance
...<li><p>Part2: <b style="color: #4472C4">PttB344_HrpS_PJ23105_ttrR</b>,
sensing
12 KB (1,301 words) - 14:05, 19 October 2021
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...ainties as the FsrC protein, which is an important component of the quorum-sensing system is a membrane protein, and due to the structural difference of cell
19 KB (3,139 words) - 04:34, 18 October 2021
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...quence, a F1 origin, and a kanamycin resistance. To be able to combine the sensing part with the reporter part of our sensor, we need to change the T7 promote
...rocio R, Certo M, Wellington H, et al. Synthetic Cassettes for pH-Mediated Sensing, Counting, and Containment. Cell Rep. 2020 Mar 3;30(9):3139–48.e4.</s
38 KB (4,814 words) - 14:24, 21 October 2021
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...e—a biomarker for inflammation—with high specificity, using the TtrS/R sensing system. This detection will result in the production of ARG gas vesicles in
...two-component ThsS/R system functions in a similar fashion to the current sensing part as a transmembrane-regulator system, but for the detection of thiosulf
39 KB (5,290 words) - 14:12, 21 October 2021
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...lecules</b> through a cell lysis protein, which is activated by the Quorum sensing mechanism that triggers cell lysis.</p>
...ents greater stability. Our oscillator as a release system based on quorum sensing and finally the development of a simple inhibition assay, Agrobactory demon
18 KB (2,543 words) - 01:21, 22 October 2021
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...time-consuming and can only be limited to the laboratory. Since the quorum-
sensing signal system of P. aeruginosa has been well studied, the detection of its
<p style="line-height:1.5;text-align:center;"><font size="4.5">Fig 3.Quorum
sensing system of Pseudomonas Aeruginosa</p><br>
14 KB (1,996 words) - 14:37, 21 October 2021
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...uojin"> 1.IL-6 sensor 1 and IL-6 sensor 2: The IL-6 sensors are capable of sensing the IL-6 levels and releasing transcriptional factors to control the downst
...e, Matthew Thomson, and Wendell A. Lim. 2016. 'Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors', Cell, 164: 780-91.
43 KB (5,859 words) - 02:51, 22 October 2021
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...veral approaches were considered when developing a biosensor with adequate sensing capability for detecting
and allow for the steps of sulfide oxidation, and RSS sensing to be separated on a microfluidic chip. First
25 KB (3,470 words) - 17:42, 20 October 2021
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on last year's experience. The pH-sensing promoters used in the project of SZU-CHINA this year, and
...B)promoters used in our school's team project in 2019 happens to be the pH-sensing promoters.
43 KB (3,899 words) - 06:19, 19 November 2021
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<li>Phase 1 : Sensing of vitamin B12 and production of lactate</li>
<h2 style="font-size: 30px;line-height: initial;">Phase 1 : Sensing of vitamin B12 and production of lactate</h2>
26 KB (3,999 words) - 02:13, 22 October 2021
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<td class="tg-2p2w">We have shown that we were able to reproduce the sensing and reporter parts of our system, successfully transformed our system in t
<td class="tg-0lax">We made a computational model of the TtrS/R sensing system, in combination with the ARG system, to gain insight into the cascad
14 KB (1,825 words) - 10:43, 21 October 2021
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...tion of Nisin PV and DNaseI under the regulation of AIP-I regulated quorum-
sensing unit consisting of AgrC-AgrA signaling components.Inorder to develop of the
<p>The quorum
sensing molecule of S aureus ,AIP-1 regulates the activity of P2 and P3 promoter.We
35 KB (4,263 words) - 02:42, 22 October 2021
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...ent., and keep continuous communication with other schools that use quorum
sensing system. Please see the <a href="https://2021.igem.org/Team:NEU_CHINA/Desig
23 KB (2,671 words) - 17:31, 21 October 2021
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<h3>ChiSPY (Chitin-Sensing Pathway) Biosensor Device</h3>
...canonical membrane-embedded one-component system. This system can act as a sensing pathway of a specific molecule to turn on transcription to produce a desire
14 KB (1,935 words) - 03:06, 18 December 2021
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...to collect cell samples for RNA-seq. She also tested one of the heat-shock sensing circuits. In drylab, she helped complete and review a database of orthogona
...hree levels of the central dogma. She designed the <i>dnaKJ</i> heat-shock sensing circuit and was in charge of functionally testing it. Though unsuccessful,
16 KB (2,494 words) - 00:23, 18 December 2021
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..., we decided that it is safer to identify the genes responsible for quorum sensing in bacteriophages and remove them.</li>
67 KB (6,351 words) - 09:57, 17 September 2021
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...ost_type menu-item-object-page menu-item-159"><a href="./sensing">Pathogen Sensing</a></li>
...answered our questions concerning the possible difficulties of our quorum sensing molecule (AHL) passing through the Gram-positive cell wall of <em>B. subtil
34 KB (4,881 words) - 17:18, 13 December 2021
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...rgin-bottom: 0; display: flex; color: #41c1ff;">Switch activated by quorum sensing <span style="padding: 0; margin: 0; font-size: 14px;"> (Silpe, J. E.
...li id="cit2">Silpe, J. E., & Bassler, B. L. (2019). A Host-Produced Quorum-Sensing Autoinducer Controls a Phage Lysis-Lysogeny Decision. <i>Cell</i>, 176(1-2)
77 KB (7,319 words) - 13:33, 8 December 2021
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...he Quorum sensing pathway of S.aureus. S.aureus produces AIP-I as a quorum-sensing molecule. So, we choose to incorporate the agrA_agrC system to enable our G
25 KB (3,176 words) - 02:35, 22 October 2021
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...pe out of the cell and kill the pathogenic cells) downstream to the quorum sensing unit. After this basic design of our genetic circuit, we had a conversation
1.02 MB (54,624 words) - 22:40, 19 October 2021
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...y the Killer X will be released as long as the bacteria receive the quorum-sensing signal DSF. This project can be separated into four different groups and w
3: DSF sensor system: Quorum sensing system in the Xanthomonas will activate by DSF molecule. The influence in c
10 KB (1,354 words) - 03:54, 22 October 2021
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...blank" href= "http://parts.igem.org/Part:BBa_K3972001">BBa_K3972001</a>)
sensing part and the ARG1 (<a class="pagelink" target=" blank" href= "http://parts
<p style="font-size:90%">The system from tetrathionate
sensing to gas vesicle formation functions the same as design A, however, only two
16 KB (1,998 words) - 09:38, 21 October 2021
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...oject is inspired by the study‘A Modular Receptor Platform To Expand the Sensing Repertoire of Bacteria’ (Chang et al, 2017), and the iGEM project NANOFLE
...Cohen-Gonsaud, and J. Bonnet, “A modular receptor platform to expand the sensing repertoire of bacteria,” <i>ACS Synthetic Biology</i>, vol. 7, no. 1, pp.
18 KB (2,532 words) - 17:18, 21 October 2021
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..." rel="footnote"><span aria-label="T. Hon et al., “A mechanism of oxygen sensing in yeast. Multiple oxygen-responsive steps in the heme biosynthetic pathway
...margin-left: 10px;">K. E. Kwast, P. V. Burke, and R. O. Poyton, “Oxygen sensing and the transcriptional regulation of oxygen-responsive genes in yeast,”
12 KB (1,673 words) - 03:08, 22 October 2021
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...r",null),"[5].Rutherford, S. T., & Bassler, B. L. (2012). Bacterial quorum sensing: its role in virulence and possibilities for its control. Cold Spring Harb
25 KB (3,950 words) - 02:44, 25 September 2021
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...diagnosis of gut inflammation through nitrate sensing[J]. Biosensors and Bioelectronics,
gut inflammation through nitric oxide sensing[J]. ACS synthetic biology, 2012, 1(10):
65 KB (5,869 words) - 02:11, 18 November 2021
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thiosulfate sensing system. </div>
sensing system’s working condition are seriously affected by the strength promote
96 KB (9,270 words) - 12:37, 16 December 2021
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...he potential to overturn the fashion industry; more recent advances in bio-sensing and processing are producing solutions to real world problems such as pollu
...ang, T.-C. et al. Hydrogel-based biocontainment of bacteria for continuous sensing and computation. Nat Chem Biol 1–8 (2021) doi:10.1038/s41589-021-00779-6.
25 KB (2,948 words) - 14:02, 20 November 2021
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...ed that Hidro can be implemented in diverse scenarios, such as heavy metal sensing, food-quality detection, drug secretion, etc. </p>
<h2 style="display:inline-block;">Sensing</h2>
19 KB (2,107 words) - 15:53, 21 October 2021
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...ost_type menu-item-object-page menu-item-159"><a href="./sensing">Pathogen Sensing</a></li>
...major part of our project was the design of a genetic circuit that allowed sensing of certain pathogens by responding to specific signaling molecules. Accordi
38 KB (5,213 words) - 16:28, 20 October 2021
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...ne remote sensing observations. ISPRS Journal of Photogrammetry and Remote Sensing 171: 36-48. (2021)</p>
15 KB (2,198 words) - 16:49, 17 December 2021
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...95;The total amount of AHL was composed of the initial AHL from the quorum sensing model. The AHL-LuxR complex would activate the Plux promoter , which could
...wanted to predict the production of these proteins. Considering the Quorum Sensing Model, we constructed the simulation of BMP2 and STATH.<br>
33 KB (3,693 words) - 01:19, 22 October 2021
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...:white; font-family:monserrat !important;">PROTEASE PRODUCTION AND HORMONE SENSING</h5>
...">The steps followed by the Genetically Modified Organism are progesterone sensing and cloaking the ovum from the sperm by zona hardening. </p>
45 KB (5,065 words) - 03:12, 17 December 2021
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The second idea we decided on, was to use quorum sensing to establish a minimum level for cry toxin expression. We decided against t
· Enhancing soil microbiome and nutrient sensing through root exudates </br> \
21 KB (1,969 words) - 00:36, 22 October 2021
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...ors and transport signaling to the downstream components. LuxI/LuxR quorum sensing system from <span class="ital">E. coli</span> and Hrp. amplifier from Pse
Thanks to iGEM team BS_United_China for your help and suggestions on quorum sensing system.
20 KB (2,388 words) - 17:35, 21 October 2021
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...ent., and keep continuous communication with other schools that use quorum
sensing system. Please see the <a href="https://2021.igem.org/Team:NEU_CHINA/Desig
23 KB (2,691 words) - 03:26, 22 October 2021
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...rum sensing</strong>: Parts involved in intercellular signaling and quorum sensing among bacteria are not harmful for humans or other organisms. <br>
12 KB (1,436 words) - 02:54, 22 October 2021
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...out the influence that the <b>different RBS</b> exerts towards the nitrate sensing system. The results indicates that, <b>when we have the similar promoter al
...he influence that the <b>different promotor</b> exerts towards the nitrate sensing system. The results indicates that, <b>when we have the similar RBS along w
42 KB (4,212 words) - 04:08, 18 November 2021
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...ical molecules is called quorum sensing. Through over expression of quorum sensing system in electrogenic bacteria, we greatly improved the efficiency of fuel
41 KB (6,014 words) - 14:27, 21 October 2021
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...ical molecules is called quorum sensing. Through over expression of quorum sensing system in electrogenic bacteria, we greatly improved the efficiency of fuel
64 KB (9,341 words) - 02:18, 22 October 2021
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...ical molecules is called quorum sensing. Through over expression of quorum sensing system in electrogenic bacteria, we greatly improved the efficiency of fuel
22 KB (3,055 words) - 01:26, 22 October 2021
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sensing phenomenon studies.
the quorum sensing additional approach and validated our population model</span>
182 KB (15,883 words) - 03:56, 18 December 2021
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...in should be killed to prevent them from propagating. We designed a quorum
sensing Kill switch which only allows cells to survive under conditions of high cel
...nterview with Prof. Fisher. But we think our constructs will well adapt to
sensing other Bd specific molecules. More at <a href="https://2021.igem.org/Team:KU
9 KB (1,366 words) - 22:35, 21 October 2021
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...o use for LuxR and LuxI expression. Therefore, we decided to create quorum sensing models with different combination of promoters(J23100,J23106) and RBS(B0034
5 KB (830 words) - 01:53, 22 October 2021
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DBTL cycle of fatty acids sensing module
<h2>DBTL cycle of fatty acids sensing module</h2>
52 KB (5,798 words) - 02:32, 22 October 2021
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...molecules is called quorum sensing. Through overexpression of quorum sensing system in
31 KB (3,497 words) - 02:52, 22 October 2021
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...ign-j"]}]},{"pos":"maintext2","word":[{"char":" In the process of quorum sensing, the threshold of the number of bacteria, that is T0 at a certain time in
59 KB (7,755 words) - 11:56, 21 October 2021
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<h4>Progesterone sensing</h4>
...enter">Working of the genetic circuit for ovastacin production and hormone sensing.
57 KB (6,516 words) - 03:13, 17 December 2021
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...sing for intestinal chloride concentration and pathogenic bacterium quorum-sensing
7 KB (823 words) - 20:34, 21 October 2021
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...s, kynurenic acid and tryptamine, to find out if our capsule is capable of sensing different metabolite concentrations in the gut lumen. We also conducted a d
24 KB (3,217 words) - 22:56, 21 October 2021
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...td>TEF1p-Estra-ScADH1t</td><td>Sequence constituively expressing estradiol-sensing protein required for estradiol induction</td><td>Composite part </td></t
10 KB (1,359 words) - 21:31, 21 October 2021
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...ield where they could sense pathogens attacking the plant via their quorum-sensing molecules and secrete the immunogenic OMVs as a response to the attack.
31 KB (4,456 words) - 21:47, 20 October 2021
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...uojin"> 1.IL-6 sensor 1 and IL-6 sensor 2: The IL-6 sensors are capable of sensing the IL-6 levels and releasing transcriptional factors to control the downst
...e, Matthew Thomson, and Wendell A. Lim. 2016. 'Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors', Cell, 164: 780-91.
42 KB (5,827 words) - 16:29, 18 October 2021
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..., we decided that it is safer to identify the genes responsible for quorum sensing in bacteriophages and remove them.</li>
54 KB (6,840 words) - 20:55, 21 October 2021
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...ivator SoxR to our biobrick for increased function of the oxidative stress
sensing system<sup>[<a href="#ref3">3</a>]</sup>.
13 KB (1,276 words) - 03:50, 14 December 2021
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...L). In the natural environment, the TolB encoding gene regulated by quorum
sensing module EsaR-PesaR (<a href='http://parts.igem.org/Part:BBa_K3989024' target
13 KB (1,837 words) - 13:27, 12 December 2021
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...rum sensing</strong>: Parts involved in intercellular signaling and quorum sensing among bacteria are not harmful for humans or other organisms. <br>
12 KB (1,430 words) - 15:49, 12 October 2021
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...e response regulator PhoB. According to Antunes et al. 2011, the phosphate sensing domain of PhoR should be replaced by a fusion of the the DHP8 region (dimer
5 KB (737 words) - 14:58, 17 December 2021
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...They are well studied PAMPs that are recognised by the receptors FLAGELLIN-SENSING 2 (FLS2) and EF-Tu RECEPTOR (EFR) respectively. We chose these elicitors be
...protein to react to QS molecules from a specific pathogen. In our design, sensing of AHL molecules would result in the repression of tolB production and expr
20 KB (2,521 words) - 08:09, 14 December 2021
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...ost_type menu-item-object-page menu-item-159"><a href="./sensing">Pathogen Sensing</a></li>
...trong>, for example our project PHIRE BYRD. Dividing the labor of the sensing of the pathogens, the production of the phages and ensuring the homogeneity
57 KB (7,738 words) - 16:29, 20 October 2021
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...l result in a GMO with sufficient production of the protein of interest or sensing abilities, while DOPL LOCK is in place to ensure the safety of the organism
...., & Wellington, H. et al. (2020). Synthetic Cassettes for pH-Mediated Sensing, Counting, and Containment. Cell Reports, 30(9), 3139-3148.e4. doi: 10.1016
70 KB (9,599 words) - 23:17, 21 October 2021
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...n talking about acne treatment, which inspired us to design the fatty acid sensing module for
the fatty acid sensing module. The improvement of the RBS and the two DBTL cycles in this part are
27 KB (2,778 words) - 03:16, 22 October 2021
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...ns and conducting our human practices, we finally decide to use the quorum sensing system [1]. "),a.a.createElement("p",null,"Las system and rhl system are th
9 KB (1,457 words) - 00:11, 22 October 2021
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...ent., and keep continuous communication with other schools that use quorum
sensing system. Please see the <a href="https://2021.igem.org/Team:NEU_CHINA/Desig
25 KB (2,871 words) - 05:37, 21 October 2021
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...e integrate the ability of QseCs to sense autoinducer-3 (AI-3), the quorum sensing molecule used by E. coli (Walters & Sperandio, 2006), but we also used coli
<li>Walters, M., & Sperandio, V. (2006). Quorum sensing in Escherichia coli and Salmonella. International Journal of Medical Microb
21 KB (2,802 words) - 03:58, 21 October 2021
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...NO<sup>3-</sup> to release the substance R; B. PttB344_HrpS_PJ23105_ttrR, sensing S<sub>4</sub>O<sub>6</sub><sup>2-</sup> to release the substance S; C. Phr
14 KB (1,584 words) - 14:04, 19 October 2021
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Quorum sensing, an intercellular communication system that is essential for biofilm format
Moreover, it inhibits quorum sensing by decreasing density of <i>S.mutans</i>.
17 KB (2,057 words) - 02:00, 22 October 2021
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...m of a flhDC promoter (BBa_K554001) in the presence of both AI-3, a quorum sensing molecule, and nitric oxide, which has a short half-life and is oxidized int
...loop design using NarX/L’s ability to activate the yeaR-yoaG operon upon sensing nitrate. Specifically, the PyeaR promoter (BBa_K216005) in this operon cont
22 KB (3,142 words) - 04:03, 21 October 2021
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...uction of an artificial metabolic burden and a theoretical tailored quorum-sensing based kill switch. Altogether these measures harbor the potential to ultima
...conceptualized a kill switch which could be activated on 1. missing quorum sensing upon eventual <i>Lactobacillus</i> escape from the biocontainer in the inte
24 KB (3,583 words) - 16:07, 16 December 2021
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of the engineered bacteria in sensing fatty acid. BBa_K3763021 is a bacteriocin that specifically
BBa_K3763002, and BBa_K3763003 are for sensing the fatty acid. BBa_K3763023 and BBa_K3763024 are for
23 KB (2,256 words) - 02:26, 22 October 2021
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Another prominent example is the detection of landmines by the way of sensing trinitrotoluene (TNT) and its degradation product, 2,3-dinitrotoluene (DNT)
...oreporters for the detection of bioavailable metals,” <em>Whole Cell Sensing System II</em>, pp. 31–63, 2009.</li>
28 KB (4,170 words) - 12:25, 20 October 2021
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<h3>External Phosphate
Sensing Promoter Sequence</h3>
<p>The External Phosphate
Sensing Promoter is a basic, regulatory part created by iGEM08_NYMU-Taipei. The ind
17 KB (2,520 words) - 00:19, 22 October 2021
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...ting a series of concentration gradients with the knowledge of that quorum sensing works after the concentration of signal molecules reaching a threshold and
11 KB (1,138 words) - 16:34, 21 October 2021
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Fatty Acids Sensing Module
<h2>Fatty Acids Sensing Module</h2>
50 KB (4,968 words) - 02:35, 22 October 2021
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...le for convenience, we will complete our design by replacing it with other sensing systems specific to IBD treatment, like sensors</p><br/>
17 KB (2,401 words) - 16:16, 21 October 2021
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<p> </p> Quorum
sensing consultation (target Vibrio cholera)
<p> </p>CAI-1 quorum
sensing modeling
9 KB (1,015 words) - 18:32, 21 October 2021
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...le for convenience, we will complete our design by replacing it with other sensing systems specific to IBD treatment, like sensors</p><br/>
17 KB (2,408 words) - 02:41, 22 October 2021
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...gulate lactate metabolism, the key point of material flux, through lactate sensing system, orthogonal targeted protease degradation, etc. Additionally, we als
9 KB (1,337 words) - 19:55, 15 March 2021
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...sponsive cellular circuits. The system we aim to design is composed of the sensing module, which may be triggered by selected molecules, light or other signal
9 KB (1,349 words) - 19:55, 15 March 2021
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...ty to fluorescence measurements—require sensors. Projects based on novel sensing technologies, or innovative remixes of current sensors, are a welcome addit
7 KB (938 words) - 16:20, 6 April 2021
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...nd burns as well as immunodeficient people. P. aeruginosa cells use quorum sensing to communicate with each other by secreting autoinducers into their environ
9 KB (1,293 words) - 19:56, 15 March 2021
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...In a small enclosed device <span class="question_note">(Examples: a bio-sensing strip with cells
98 KB (9,829 words) - 16:31, 25 March 2022
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...spread of their fungicidal bacteria, the team plans to test a quorum-sensing-based kill switch, or to place
9 KB (1,206 words) - 14:21, 7 July 2021
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<p class="text">6. Smartt AE, Ripp S. Bacteriophage reporter technology for sensing and detecting microbial targets. Anal Bioanal Chem. 2011 May;400(4):991-100
23 KB (3,287 words) - 02:01, 15 December 2021
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...bial biosensor by coupling the <i>Vibrio cholerae</i> one component chitin-sensing system with a GFP reporter in <i>Escherichia coli</i>. This biosensor will
9 KB (1,087 words) - 19:57, 18 December 2021
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...cells with higher cell densities than usual, as RelE-RelB controls quorum sensing in <i>E. coli</i>, which would have applications like growing more <i>E. co
15 KB (1,927 words) - 17:25, 21 October 2021
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...mall, urban communities by providing cost-effective and precise methods of sensing nutrient concentrations and environmental conditions. Utilizing biosensors,
22 KB (3,233 words) - 02:57, 22 October 2021
