Team:WHU-China/Parts

Parts

We use and construct parts that are considered an important part of our project, and most of them have been tested to ensure their reliability.

To efficiently obtain these parts, we asked Tsingke Biotechnology to synthesize some of them. Besides, we also used colony PCR to get several parts from the original bacteria strain directly.

You can click Design to learn more about our project to see their specific function.


Basic Parts

Among the basic parts designed this year by ourselves, BBa_K3763000 and BBa_K3763021 are our favorite. BBa_K3763000 is an RBS improved based on BBa_B0030, which improves the performance of the engineered bacteria in sensing fatty acid. BBa_K3763021 is a bacteriocin that specifically targets Propionibacterium bacteria, including Propionibacterium acnes.

Basic Parts
Name Description Type Length
BBa_I746361 PO promoter Promoter 92bp
BBa_K906007 pBAD promoter Promoter 345bp
BBa_K3805208 trc promoter Promoter 30bp
BBa_K3040118 FadD Coding 1786bp
BBa_K3763042 FadE Coding 2445bp
BBa_B0010 rrnB T1 terminator Terminato 86bp
BBa_K3763043 rrnB T2 terminator Terminato 28bp
BBa_I0500 araBAD promoter Promoter 166bp
BBa_K861050 FadR Coding 720bp
BBa_B0010 rrnB T1 terminator Terminator 87bp
BBa_K3040114 pFadD_Lac Promoter 279bp
BBa_B0030 RBS 99-2(origin) RBS 15bp
BBa_B0010 rrnB T1 terminator Terminator 87bp
BBa_K3763000 RBS improved on the basis of BBa_B0030 RBS 15bp
BBa_K3763021 PctA gene Coding 288bp
BBa_K3763022 coding sequence of mature PctA protein Coding 195bp
BBa_K387007 OmpA leader peptide Signaling 63bp
BBa_E0040 GFP Coding 720bp


Composite Parts

We have designed a series of composite parts this year and all of them have been tested. BBa_K3763044 and BBa_K3763045 are for the consumption of fatty acid. BBa_K3763001, BBa_K3763002, and BBa_K3763003 are for sensing the fatty acid. BBa_K3763023 and BBa_K3763024 are for the inhibition of the growth of Propionibacterium acnes.

Composite Parts
Name Description Type Length
BBa_K3763044 araBAD promoter + FadE + rrnB T1 terminator + rrnB T2 terminator Composite 3233bp
BBa_K3763045 trc promoter + FadD + rrnB T1 terminator + rrnB T2 terminator Composite 2240bp
BBa_K3763001 araBAD promoter-FadR-rrnB T1 terminator Composite 1534bp
BBa_K3763002 pFadD_Lac-RBS BBa_B0030-GFP-rrnB T1 and T2 terminator Composite 1445bp
BBa_K3763003 pFadD_Lac-RBS BBa_K3763000-GFP-rrnB T1 and T2 terminator Composite 1445bp
BBa_K3763023 T7 promotor-lac operator-RBS 1-Sec leader peptide-PctA CDS- 6*His tag-T7 terminator Composite 517bp
BBa_K3763024 T7 promotor-lac operator-RBS 1-OmpA leader peptide-PctA CDS- 6*His tag-T7 terminator Composite 487bp