Team:TU-Eindhoven/Proof Of Concept

Proof of Concept

Since the current methods for monitoring IBD patients are invasive, complex, non-specific, expensive, and time-consuming, we developed IBDetection. We envision a pill that deliberately releases Genetically Modified Organisms (GMOs), which sense tetrathionate and produces gas vesicles, into the intestine [1,2]. To proof IBDetection, we characterized the tetrathionate sensing TtrS/R system, reproduced the ultrasound-based ARG1 reporter system, and transformed the entire sensor required for IBDetection into three different E. coli strains, including BL21 (DE3) [3].