Important parts
Our induction system
The induction system are responsible for inducing expression of our desired proteins upon addition of the corresponding induction agent. The copper system is native to yeast and responds to CU2+ ions. The TetOn system is from bacterial antibiotic resistance and activates upon addition of tetracycline family antibiotics, requiring the presence of the rtTA-NLS-GAL4AD fusion protein, known as TetA.
A Table Shows Parts of The Induction System
Biobrick ID | Short name | Description | Type |
---|---|---|---|
BBa_K3944007 | CUP1p + miRFP | Sequence expressing miRFP upon copper addition for copper induction benchmarking | Composite part |
BBa_K3944008 | CUP1p + TesBT | Sequence expressing TesBT upon copper addition for fatty acid synthesis control, producing short fatty acids | Composite part |
BBa_K3944019 | TetO7+ScPHO5+mTagBFP2 | Sequence expressing mTagBFP2 upon tetracycline addition, for tetracycline induction benchmarking | Composite part |
BBa_K3944020 | TetO7+ScPHO5+TesA | Sequence expressing TesA upon tetracycline addition for fatty acid synthesis control, producing medium fatty acids | Composite part |
BBa_K3944021 | TEF1p + TetA + ScPGK1 | Sequence constituively expressing tetracycline-sensing protein required for tetracycline induction | Composite part |
BBa_K3944032 | ScGAL1 + Z3BS - GFP | Sequence expressing GFP upon estradiol addition, for estradiol induction benchmarking | Composite part |
BBa_K3944033 | ScGAL1 + Z3BS - FatB | Sequence expressing FatB upon estradiol addition for fatty acid synthesis control, producing long fatty acids | Composite part |
BBa_K3944035 | TEF1p-Estra-ScADH1t | Sequence constituively expressing estradiol-sensing protein required for estradiol induction | Composite part |
The yeast fatty acid synthase (FAS) system
The yeast fatty acid synthase (FAS) system cannot be targeted for protein engineering efficiently since it is expressed as two large genes that complex to provide function, so we replace it with a bacterial FAS2 system consiting of these 8 genes.
Estradiol signalling comes homo sapiens and is a hormone signalling system, responding to the hormone estradiol.
A Table Shows The yeast fatty acid synthase (FAS) system
Biobrick ID | Short name | Description | Type |
---|---|---|---|
BBa_K3944038 | AcpP | AcpP enzyme coding sequence | Basic part |
BBa_K3944039 | AcpS | AcpS enzyme coding sequence | Basic part |
BBa_K3944040 | FabB | FabB enzyme coding sequence | Basic part |
BBa_K3944041 | FabH | FabH enzyme coding sequence | Basic part |
BBa_K3944042 | FabD | FabD enzyme coding sequence | Basic part |
BBa_K3944043 | FabZ | FabZ enzyme coding sequence | Basic part |
BBa_K3944044 | FabG | FabG enzyme coding sequence | Basic part |
BBa_K3944045 | FabI | FabI enzyme coding sequence | Basic part |
Improvement Of Parts——Ribosome-Binding Sites
As a part of our committment to future iGEM teams, we also characterised three ribosome binding sites (RBS) to provide additonal knowledge and options to future teams.
The characterisation was done on parts previously reported by other teams in the part registry.
A Table Shows our Ribosome-Binding Sites
Biobrick ID | Short name | Description | Type |
---|---|---|---|
BBa_K3252027 | BBa_K3252027 | Weak RBS site | |
BBa_K3252026 | BBa_K3252026 | Medium RBS site | |
BBa_J15001 | BBa_J15001 | Strong RBS site |
All Parts
Here is a list of all basic parts and composite we created.
<groupparts>iGEM21 Chalmers-Gothenburg</groupparts>