Team:CSU CHINA/Best Basic Part



GIP promoter is a glucose induced promoter, the reasons why we think it can be regarded as the best basic part are as followed:

(1)GIP promoter used to be assumed that it can only be used in several certain types of cells like intestine cells, but we have proved that it can function well in 293T, a type of cell usually used in labs. We have increased its universality, this is a breakthrough we have made on current literature

(2)The abnormality of glucose concentration can be important to lots of diseases, as for synthetic biology, the regulation of blood sugar is meaningful. It has a wide range of applications. However, current regulators are mostly lactose or other substances and current glucose induced promoter is few. We think these 2 glucose induced promoter can provide help for future iGEM teams.

(3)We designed different glucose concentration gradient through real-time LUC and sifted the best concentration, laid a foundation for future iGEMers’ experiments.

2.Pattern diagram

Fig.1The model diagram of GIP-miR21T-GI-GAL4-4XmiR21T


3.1 Method

We use LUC as report genes to reflect the level of expression through detecting luminescence value at 560nm wavelength and the error REN luminescence caused by the number of eliminated cells

3.2 Result

GIP promoter is induced by glucose and the expression rises along with the raise of glucose concentration.

Fig.2 Changes of LUC expression over time after blue light irradiation with different glucose concentrations


Use medium with no glucose when during the cell paving in case glucose left effect the following experiment.


[1]Anthony T. Cheung,Bama Dayanandan,Jamie T. LewisGlucose-Dependent Insulin Release from Genetically Engineered K Cells[J].Science.2000,290:1959-1962.