Team:BUCT/Engineering
Overview
"We followed the engineering cycle for countless iterations over the course of our project.
1. 5HTP synthesis
2. Enhances beta oxidation
3. Oxygen-regulated suicide
Please review the Result page for more information
References
1. Lin Y, Sun X, Yuan Q, Yan Y. Engineering bacterial phenylalanine 4-hydroxylase for microbial synthesis of human neurotransmitter precursor 5-hydroxytryptophan. ACS Synth Biol. 2014 Jul 18;3(7):497-505. doi: 10.1021/sb5002505. Epub 2014 Jun 30. PMID: 24936877.
2. Wang H, Liu W, Shi F, Huang L, Lian J, Qu L, Cai J, Xu Z. Metabolic pathway engineering for high-level production of 5-hydroxytryptophan in Escherichia coli. Metab Eng. 2018 Jul;48:279-287. doi: 10.1016/j.ymben.2018.06.007. Epub 2018 Jun 19. PMID: 29933064.
3. Luo H, Schneider K, Christensen U, Lei Y, Herrgard M, Palsson BØ. Microbial Synthesis of Human-Hormone Melatonin at Gram Scales. ACS Synth Biol. 2020 Jun 19;9(6):1240-1245. doi: 10.1021/acssynbio.0c00065. Epub 2020 Jun 5. PMID: 32501000.
4. Chae TU, Ko YS, Hwang KS, Lee SY. Metabolic engineering of Escherichia coli for the production of four-, five- and six-carbon lactams. Metab Eng. 2017 May;41:82-91. doi: 10.1016/j.ymben.2017.04.001. Epub 2017 Apr 5. PMID: 28390749.
5. Wu H, Wang H, Chen J, Chen GQ. Effects of cascaded vgb promoters on poly(hydroxybutyrate) (PHB) synthesis by recombinant Escherichia coli grown micro-aerobically. Appl Microbiol Biotechnol. 2014 Dec;98(24):10013-21. doi: 10.1007/s00253-014-6059-y. Epub 2014 Sep 13. PMID: 25216582.
6. J. Agric. Food Chem. 2017, 65, 8, 1669–1676Publication Date:February 2, 2017https://doi.org/10.1021/acs.jafc.6b05237
7. Nikolic, N., Bergmiller, T., Vandervelde, A., Albanese, T. G., Gelens, L., & Moll, I. (2018). Autoregulation of mazEF expression underlies growth heterogeneity in bacterial populations. Nucleic acids research, 46(6), 2918–2931. https://doi.org/10.1093/nar/gky079
8. Engelberg-Kulka, H., Hazan, R., & Amitai, S. (2005). mazEF: a chromosomal toxin-antitoxin module that triggers programmed cell death in bacteria. Journal of cell science, 118(Pt 19), 4327–4332. https://doi.org/10.1242/jcs.02619
9. Hazan R, Sat B, Engelberg-Kulka H. Escherichia coli mazEF-mediated cell death is triggered by various stressful conditions. Journal of Bacteriology. 2004 Jun;186(11):3663-3669. DOI: 10.1128/jb.186.11.3663-3669.2004. PMID: 15150257; PMCID: PMC415763.
2. Wang H, Liu W, Shi F, Huang L, Lian J, Qu L, Cai J, Xu Z. Metabolic pathway engineering for high-level production of 5-hydroxytryptophan in Escherichia coli. Metab Eng. 2018 Jul;48:279-287. doi: 10.1016/j.ymben.2018.06.007. Epub 2018 Jun 19. PMID: 29933064.
3. Luo H, Schneider K, Christensen U, Lei Y, Herrgard M, Palsson BØ. Microbial Synthesis of Human-Hormone Melatonin at Gram Scales. ACS Synth Biol. 2020 Jun 19;9(6):1240-1245. doi: 10.1021/acssynbio.0c00065. Epub 2020 Jun 5. PMID: 32501000.
4. Chae TU, Ko YS, Hwang KS, Lee SY. Metabolic engineering of Escherichia coli for the production of four-, five- and six-carbon lactams. Metab Eng. 2017 May;41:82-91. doi: 10.1016/j.ymben.2017.04.001. Epub 2017 Apr 5. PMID: 28390749.
5. Wu H, Wang H, Chen J, Chen GQ. Effects of cascaded vgb promoters on poly(hydroxybutyrate) (PHB) synthesis by recombinant Escherichia coli grown micro-aerobically. Appl Microbiol Biotechnol. 2014 Dec;98(24):10013-21. doi: 10.1007/s00253-014-6059-y. Epub 2014 Sep 13. PMID: 25216582.
6. J. Agric. Food Chem. 2017, 65, 8, 1669–1676Publication Date:February 2, 2017https://doi.org/10.1021/acs.jafc.6b05237
7. Nikolic, N., Bergmiller, T., Vandervelde, A., Albanese, T. G., Gelens, L., & Moll, I. (2018). Autoregulation of mazEF expression underlies growth heterogeneity in bacterial populations. Nucleic acids research, 46(6), 2918–2931. https://doi.org/10.1093/nar/gky079
8. Engelberg-Kulka, H., Hazan, R., & Amitai, S. (2005). mazEF: a chromosomal toxin-antitoxin module that triggers programmed cell death in bacteria. Journal of cell science, 118(Pt 19), 4327–4332. https://doi.org/10.1242/jcs.02619
9. Hazan R, Sat B, Engelberg-Kulka H. Escherichia coli mazEF-mediated cell death is triggered by various stressful conditions. Journal of Bacteriology. 2004 Jun;186(11):3663-3669. DOI: 10.1128/jb.186.11.3663-3669.2004. PMID: 15150257; PMCID: PMC415763.
The whole BUCT team would like to thank our sponsors. Especially:
Tel: 86+18718806286
Address: No.15 North Third Ring East Road,Chaoyang District,Beijing,P.R.China. Beijing University of Chemical Technology
BUCT--iGEM
BUCT--iGEM
