Team:Tsinghua/Protocol

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Protocol

biochemistry experiment

Dot_blot_analysis_of_in_vitro_RNA_labeling
Protein_Expression_and_Purification
Western_Blot

molecular experiment

RNA_in_vitro_transcription_using_T7_RNA_Polymerase
SDS-PAGE
Base-editing_system
sgRNA_coloning
dCas9_strain_construction
E.coli%40PCS_preparation
in_vitro_validation_by_fluorescence
In_vitro_validation_by_digestive_juice
Agarose_gel_electrophoresis
Clony_PCR_system%2820%CE%BCL%29
E.coli%40PCS_preparation
Electroporation_competent_EcN_preparation
preparation_of_competent_state_and_the_electroporation_of_L.Lactis
Electrotransformation_%28for_EcN%29

cell experiment

cell_experiment

animal experiment

colon_length_test
weight
DSS_induction
mouse_management
Intragastric_administration
Fecal_occult_blood_test

microbiology experiment

Co-culture_analysis
Growth_curve_measurement_of_L.lactis

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