Team:Tsinghua/Attributions

Attributions

Team members

Table.1 Team attributions

PIs

Professor Guoqiang Chen: Primary PI, provided instructions to general design; assisted us in animal protocol examination; provided some reagents for ourexperiments.

Doctor Peng Li: Secondary PI & supervisor of our team; provided instructions to project and experiment design; organized teamwork trainings; helped us greatly with lab management and safety.

Prof. Zhirong Sun: Supervised the process of our project and provided suggestions on project.

Advisors

Xunxun Luo: Graduate student from Prof. Xinxin Hui's lab; provided EcN strain and backbone plasmids; helped with our experiment design, specifically the electro-transformation.

Doctor Xuan Wang: Graduate student from Prof. Guoqiang Chen's lab; helped with our experiments and project design; provided reagents like Streptavidin-HRP.

Yudian Zhang: Graduate student from Prof. Guoqiang Chen's lab; helped with our cell assays, WB assays, and animal assays (including guidance on animal form, providing kits for fecal occult blood, etc.).

Qinglin Zeng: Undergraduate student, 2020 Tsinghua iGEM team leader; provided guidance on project design and iGEM competition.

Xiaofei Ge: 2019 Tsinghua iGEM team member; provided guidance on project design and iGEM competition.

Ao Wang: 2019 Tsinghua iGEM team member; provided guidance on project design and iGEM competition.

Junlin Li: 2019 Tsinghua iGEM team member; helped us get familiar with iGEM competition.

Yufan Yang: 2019 Tsinghua iGEM team member; helped us get familiar with iGEM competition.

Zijing Zhou: 2019 Tsinghua iGEM team member; helped us get familiar with iGEM competition.

Financial Support

Tsinghua Xuetang Life Science Program and Tsinghua-Peking joint Center of Life Science (CLS).

Labs

Guoqiang Chen's lab (School of Life Sciences, Tsinghua University): Provided reagents such as testing kit for fecal occult blood and certain culture media; provided plasmids encoding IBD sensors; our animal experiments are a part of Chen lab's research.

Xinhui Xing's lab (Department of Chemical Engineering, Tsinghua University): Provided EcN strain and EcN medium-copy plasmid; provided instructions to the electroporation of EcN.

Deng Pan's lab (School of Medicine, Tsinghua University): Provided instructions to FACS analysis; our cell culturing experiments were conducted in Prof. Pan's lab.

Li Yu's lab (School of Life Sciences, Tsinghua University): Provided great assistance in WB.

Peng Zou's lab (College of Chemistry and Molecular Engineering, Peking University): Provided sequence of miniSOG; provided great assistance in RNA purification.

Special Thanks

Professor Wei Wu (School of Life Sciences, Tsinghua University): Advice on proof-of-concept animal experiments.

Researcher Xia Wang (School of Pharmaceutical Sciences, Tsinghua University): Advice on proof-of-concept animal experiments.

Professor Xin Liang (School of Life Sciences, Tsinghua University): Advice on experiments and communications.

Researcher Jinyao Liu (Shanghai Jiao Tong University): Instructions to surface decoration of bacterial cells.

Doctor Zai Chang (executive deputy director of Laboratory Animal Resources Center, Tsinghua University): Assistance in animal protocol examination.

Mr. Canhui Lan (initiator & executive chairman of ChinaGut Festival): Provided opportunity of attending ChinaGut Festival.

Professor Jianbin Wang (School of Life Sciences, Tsinghua University): Provided ways of communication for the diagnostics part of our project.

Researcher Peng Zou (IDG/McGovern Institute for Brain Research at Peking University): Provided sequence of miniSOG.

Doctor Xiaoyan Tang (Peking Union Medical College Hospital): Instrutions to the questionnaire on breast feeding.

Doctor Yue Li (Peking Union Medical College Hospital): Information about current diagnosis and treatment methods of IBD.

Doctor Chao Pao (Post-doc in Shanghai Institute of Materia Medica., Chinese Academy of Sciences): Instructions to surface decoration of bacterial cells.

Doctor Xu Yan (doctor in Chen Lab, School of Life Sciences, Tsinghua University): Assistance in L.lactis electroporation and animal protocol.

Doctor Dong Jiang (Post-doc in Yu Lab, School of Life Sciences, Tsinghua University): Assistance in WB.

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