KORHS
PART COLLECTION
Basic Information:
The function of Parts Collection is as follows: (1) characterization of anti-endotoxin and anticancer cell-penetrating peptide buforin IIb from antimicrobial peptide buforin family; (2) modification of buforin IIb (MV1 and MV2) for maintenance of cancer specificity and minimization of cytotoxicity; (3) synthetic construct of BIIB/MV1/MV2-siRNA for RISC-induced sequence-specific silencing of CYP1A1; (4) CYP1A1 cDNA-specific amplification via RT-PCR for the analysis of construct transfection and gene silencing efficacy.
Parts Collection
- Buforin IIb: BBa_K3824000
- Modified Version 1 (MV1) peptide: Modified Buforin IIB (-RLLR): BBa_K3824001
- Modified Version 2 (MV2) peptide: Modified Buforin IIB (-RLLRRLLR): BBa_K3824002
- siRNA targeting CYP1A1: BBa_K3824003
- CYP1A1 RT-PCR primer: BBa_K3824004