Team:Korea HS/Notebook
KORHS
Team Korea_HS
Date : March 9th, 2021
Event : 1st Virtual Meeting
Agenda : The team was introduced to its members (for the first time), 2021 research idea (by Professor Lee), and iGEM competition logistics (by advisors Sam Han and Jina Bae).
Date : April 17th, 2021
Event : 2nd Virtual Meeting
Agenda : The team discussed MV1/MV2-siRNA-doxorubicin construct in depth and was introduced to team divisions (Wiki, Human Practices, and Collaboration).
Date : May 1st, 2021
Event : 3rd Virtual Meeting
Agenda : The team discussed fundamental concepts of (normal and cancer) cell lines and siRNA and was engaged in the first sub-team (Wiki, Human Practices, and Collaboration) discussions.
Date : May 15th, 2021
Event : 4th Virtual Meeting
Agenda : The team discussed experimental methods (e.g. FITC/MTT assay) of validating CPP construct penetration specificity and efficacy and elected sub-team leaders and co-leaders.
Date : May 29th, 2021
Event : 5th Virtual Meeting
Agenda : The team discussed experimental methods (e.g. RT-PCR and western blotting) of validating construct transfection and gene silencing efficacy and elected main leaders.
Date : June 12th, 2021
Event : 6th Virtual Meeting
Agenda : The team discussed proof of concept methods (e.g. immunogenicity and serum degradation) with Professor Lee and scheduled wet lab meetings at Suwon University.
Date : June 26th, 2021
Event : 7th Virtual Meeting
Agenda : The team finalized proof of concept methods (e.g. modification of CPP) and wet lab meetings at Suwon University and engaged in sub-team breakout sessions.
Date : July 11th, 2021
Event : 8th Virtual Meeting
Agenda: The team discussed dry lab methods (e.g. PEP-FOLD3 and Benchling) to stimulate CPP constructs and medal criteria (particularly gold) and special/community-based awards for 2021.
Date : July 25th, 2021
Event : 9th Virtual Meeting
Agenda : The team discussed all medal and special/community-based award criteria and workload allocation until Wiki Freeze (and further task assignments) and took team photos virtually.
Date : August 14th, 2021
Event : 10th Meeting (1st in-person meeting)
Agenda : The team met for the first time in-person, took team photos, discussed workload allocation and deadlines until Wiki Freeze, and completed several team tasks.
Date : August 29th, 2021
Event : 11th Virtual Meeting
Agenda : The team discussed experimental results and analysis (e.g. FITC, MTT, and RT-PCR) with Professor Lee and updated each sub-team with events and Wiki progress.
Date : September 18th, 2021
Event : 12th Meeting (2nd in-person meeting)
Agenda : The team created lab kits for elementary & middle school lab kit webinars and board and engagement materials for live sticker poll and pamphlet distribution.
Date : October 3rd, 2021
Event : 13th Virtual Meeting
Agenda : The team discussed workload distribution and deadlines for finalized Wiki and presentation video and reviewed sub-team conclusion of events and Wiki write-ups.
Date : October 16th, 2021
Event : 14th Meeting (3rd in-person meeting)
Agenda : The team concluded deadlines and the creation of final deliverables (Wiki, presentation video, and judging form) and created promotional materials for the group’s blood donation event.
Team Wet Lab
Date : July 4th, 2021
Event : 1st Wet Lab Meeting
Agenda : Students performed micropipette (Gilson) handling procedures, cell culture experiment (learning cell line and culture plate types), and trypsin cell detachment/count experiment.
Date : July 11th, 2021
Event : 2nd Wet Lab Meeting
Agenda : Students were introduced to modification of buforin IIb (MV1 and MV2) and performed colorimetric MTT metabolic (cytotoxicity) assay and stock solution (dilution) synthesis.
Date : July 18th, 2021
Event : 3rd Wet Lab Meeting
Agenda : Students were introduced to FITC constructs and performed a cell-penetration efficacy experiment by preparing stock-media mixtures with stock, RPMI media, and vortex mixer.
Date : August 1st, 2021
Event : 4th Wet Lab Meeting
Agenda : Students were introduced to siRNA + Religent synthetic constructs and performed an intracellular siRNA delivery experiment (total RNA extraction) via RNA extraction kit (Intron).
Date : August 8th, 2021
Event : 5th Wet Lab Meeting
Agenda : Students performed reverse transcription-polymerase chain reaction via RNA extraction kit, mRNA-cDNA synthesis via RTase enzyme, and CYP1A1 expression level design.
Date : August 15th, 2021
Event : 6th Wet Lab Meeting
Agenda : Students were introduced to agarose gel and completed the analysis of RT-PCR assay through quantification of CYP1A1 and GADPH band intensities and result expectations.
Team Dry Lab
Date : July 27th, 2021
Event : 1st Dry Lab Meeting
Agenda : Students discussed dry lab programs applicable to the team’s project (i.e. PEP-FOLD3, Benchling, and Ensembl) with Professor Lee and simulated peptide characterization.
Date : August 8th, 2021
Event : 2nd Dry Lab Meeting
Agenda : Students discussed applicable dry lab programs (i.e. Rosetta, Pymol, CellDesigner, and MATLAB) and their methodologies, reviewed tutorials, and sought collaboration.
Date : August 21st, 2021
Event : 3rd Dry Lab Meeting
Agenda : Students finalized simulation analysis and Wiki write-up (and workload distribution) for simulated programs and considered for future use by associating analysis with wet lab results.
Team Collaboration
Date : June 18th, 2021
Event : 1st Collaboration Meeting with IISER_Tirupati
Agenda : Both teams introduced their projects. IISER_Tirupati shared their modeling parts, and shared advice on medal criteria and human practices part.
Date : June 20th, 2021
Event : 1st Collaboration Meeting with UTEC-Peru
Agenda : Both teams presented their projects. UTEC-Peru shared suggestions for human practices and judging sessions.
Date : June 27th, 2021
Event : 1st Collaboration Meeting with IISER_Berhampur
Agenda : Both teams shared their projects. IISER_Berhampur shared their suggestions on human practices and introduced possible ideas.
Date : June 30th, 2021
Event : 1st Partnership Meeting with Greece_United (NOUS)
Agenda : Both teams shared their presentations and had a discussion about extending the collaboration to a partnership.
Date : July 1st, 2021
Event : 1st Partnership Meeting with ASIJ_Tokyo
Agenda : Both teams presented their projects and discussed possible areas for the partnership, including human practice and protein modeling.
Date : July 4th, 2021
Event : 1st Collaboration Meeting with Siberia
Agenda : Both teams introduced their projects and team Siberia advised on using longer double-stranded RNA for wet lab.
Date : July 5th, 2021
Event : 1st Collaboration Meeting with IISER_Bhopal
Agenda : Both teams shared their presentations and discussed areas of collaboration: MTT assay and protein modeling.
Date : July 9th, 2021
Event : 1st Collaboration Meeting with CCU_Taiwan
Agenda : Both teams introduced their projects and discussed areas of collaboration, wet lab, and human practices part of the project.
Date : July 10th, 2021
Event : 1st Collaboration Meeting with TU_Darmstadt
Agenda : Both teams presented their projects. Team TU_Darmstadt shared their understanding of gold medal criteria, especially, the proof of concept.
Date : July 12th, 2021
Event : 1st Collaboration Meeting with HU_Berlin
Agenda : Both teams spent time presenting their projects. HU_Berlin assisted Korea_HS with the project modeling and gold medal criteria part of iGEM.
Date : July 13th, 2021
Event : 1st Collaboration Meeting with KCIS_NewTaipei
Agenda : Both teams shared their projects, reviewed their wet-lab experiments, and discussed the project modeling part of the project.
Date : July 14th, 2021
Event : 2nd Partnership Meeting with ASIJ_Tokyo
Agenda : Both teams discussed the Northeast Asia Joint Collaboration Network, set up a possible agenda, and developed possible ideas for a YouTube promotion.
Date : July 16th, 2021
Event : 2nd Partnership Meeting with Greece_United (NOUS)
Agenda : Both teams shared ideas on ways to support one another. Greece_United shared their progress on human practices part of the project and further discussed possible areas of collaboration.
Date : July 23rd, 2021
Event : 2nd Collaboration Meeting with USP-Brazil
Agenda : Both teams suggested possible areas of collaboration and initiated collaboration on Synthetic Biology Olympics.
Date : August 4th, 2021
Event : 3rd Partnership Meeting with ASIJ_Tokyo
Agenda : Both teams proposed ideas on the YouTube promotion project and shared areas on ways to support one another.
Date : August 4th, 2021
Event : 3rd Partnership Meeting with Greece_United (NOUS)
Agenda : Both teams discussed two different projects: blood donation and co-authoring a book. Teams introduced possible ways to support the project.
Date : August 12th, 2021
Event : 4th Partnership Meeting with ASIJ_Tokyo
Agenda : Both teams shared their progress on project modeling and team Korea_HS suggested ASIJ_Tokyo to join the project “How to iGEM” of team NOUS.
Date : August 12th, 2021
Event : 4th Partnership Meeting with Greece_United (NOUS)
Agenda : Team NOUS presented on how to use MatLab, a mathematical modeling software, to Korea_HS and introduced possible modeling features for our team.
Date : August 18th, 2021
Event : 5th Partnership Meeting with ASIJ_Tokyo
Agenda : Both teams discussed the possibility of partnering to establish a shared YouTube/TikTok channel, a joint collaboration network, and a Synthetic Biology olympiad via an extended collaboration.
Date : August 23rd, 2021
Event : 2nd Collaboration Meeting with CCU_Taiwan
Agenda : Both teams discussed project progress updates, exchanged methods of entrepreneurship (e.g. market analysis), and suggested collaboration in inclusivity (LGBTQ+ in puberty).
Date : August 25th, 2021
Event : 6th Partnership Meeting with ASIJ_Tokyo
Agenda : Both teams discussed updates on Northeast Asia Joint Collaboration Network and dry lab webinars, breast cancer awareness video, and intellectual exchanges on dry and wet labs.
Date : August 25th, 2021
Event : 1st Collaboration Meeting with NYC_B1O
Agenda : Both teams discussed project ideas and discussed possible collaboration agenda on integrated human practices/education (resource exchange) and dry lab (intellectual exchange).
Date : September 1st, 2021
Event : 7th Partnership Meeting with ASIJ_Tokyo
Agenda : Both teams discussed a rough draft and participation of the 2021 Autumn Dry Lab Webinar, a shared TikTok/YouTube channel, the TAS_Taipei SDG Conference, and the Cancer Meetup.
Date : September 2nd, 2021
Event : 5th Partnership Meeting with Greece_United (NOUS)
Agenda : Both teams discussed the possibility of wet lab partnership such as exchange of specific wet-lab protocols (e.g. MTT assay and RT-PCR) and measurement and result analysis.
Date : September 3rd, 2021
Event : 2nd Collaboration Meeting with NYC_B1O
Agenda : Both teams facilitated human practices exchange (e.g. survey and stakeholders) and devised an agenda for an educational exchange program between South Korea and the U.S.
Date : September 8th, 2021
Event : 8th Partnership Meeting with ASIJ_Tokyo
Agenda : Both teams reviewed progress on interview and animation for a shared YouTube channel and finalized participation roster for Northeast Asia Joint Collaboration Network.
Date : September 9th, 2021
Event : 6th Partnership Meeting with Greece_United (NOUS)
Agenda : Both teams discussed mentored specific protocols of wet lab, such as MTT cytotoxicity assay (Korea_HS) and measurement analysis (NOUS) and devised co-authored implementation.
Date : September 11th, 2021
Event : 2nd Collaboration Meeting with IISER_Bhopal
Agenda : Both teams exchanged methods to satisfy “Partnership,” “Integrated Human Practices,” “Contribution,” “Proof of Concept,” and “Project Model” and suggested co-hosting of events.
Date : September 13th, 2021
Event : 2nd Collaboration Meeting with IISER_Berhampur
Agenda : Both teams discussed concepts of engineering cycle (process-analyze-results-improve) and application of Python and CellDesigner to modeling deterministic cell line scenarios.
Date : September 16th, 2021
Event : 7th Partnership Meeting with Greece_United (NOUS)
Agenda : Both teams reviewed the current partnership agenda and finalized workload for human practices (“How To iGEM” and “Give a Blood~Save a Life”) and the wet lab.
Date : September 17th, 2021
Event : 2nd Collaboration Meeting with Siberia
Agenda : Both teams discussed specific methods of RT-PCR band intensity quantification and FITC assay analysis and solutions to issues regarding Siberia’s genetic oscillator rate of differentiation.
Date : September 22nd, 2021
Event : 9th Partnership Meeting with ASIJ_Tokyo
Agenda : Both teams discussed the finalized script and facilitation division for the 2021 Autumn Dry Lab Webinar and 1st Conference of Northeast Asia Joint Collaboration Network.
Date : September 23rd, 2021
Event : 8th Partnership Meeting with Greece_United (NOUS)
Agenda : Both teams discussed a possible proposed implementation of fusing affinity peptide - exosome - miRNA construct with chondrocyte-specific cell-penetrating peptide KAFAK.
Date : September 29th, 2021
Event : 10th Partnership Meeting with ASIJ_Tokyo
Agenda : Both teams debriefed the 2021 Autumn Dry Lab Webinar and 1st Conference of Northeast Asia Joint Collaboration and finalized outline for partnership write-up on the Wiki.
Date : October 4th, 2021
Event : 9th Partnership Meeting with Greece_United (NOUS)
Agenda : Both teams discussed the “MATLAB Tutorial” provided by Greece_United and “Give a Blood~Save a Life” event and finalized the outline for partnership write-up on the Wiki.
Team Human Practices
Date : May 1st, 2021
Event : First HP team meeting
Agenda : Held individual introductions of sub-group members, followed by team leader elections and a short planning session.
Date : June 30th, 2021
Event : First HP interview with Dr. Sungjoon Yoon, a researcher at RWTH
Agent : Discussed theoretical clinical applicability of therapeutic and how to experimentally account for possible in vivo issues (cytotoxicity, immunogenicity in particular).
Date : July 12th, 2021
Event : Submission of experimental-tract report written by HP members to KSCY competition
Agenda: Spread awareness about our project and interacted with other students about their research via KSCY (virtual science fair/competition).
Date : July 28th, 2021
Event : Second HP interview with Dr. Munhong Kim, OB/GYN at Korea Cancer Center Hospital
Agenda : Discussed current demographics of cancer therapeutics, prospects of our peptide therapeutic, and how to account for possible in vivo deficiencies through structural modification.
Date : Aug 4th, 2021
Event : First HP interview with Dr. Youngwoong Lee, a biological science researcher at KAIST
Agenda : Discussed appropriate method of assessing product performance with special focus on cell specificity and intracellular distribution of peptides.
Date : August 14th, 2021
Event : Finalization of Korea_HS pamphlet
Agenda : Released final version of the informational pamphlet containing information about iGEM competition, cancer, and the specific project made by Ellie.
Date : August 24th, 2021
Event : Release of public engagement survey created by Andrea
Agenda: Released 8-question google form survey assessing awareness of CPP-based treatments among the public. Members recruited around 15 respondents each.
Team Education
Date : September 9th, 2021
Event : SAS-Puxi Synthetic Biology
Agenda : Haryeong introduced iGEM, Synthetic Biology, and simplified modules of the team's project to elementary school students in SAS-Puxi and also held the “Milk Lab.”
Date : September 25th, 2021
Event : 2021 Autumn Dry Lab Webinar
Agenda : Under a partnership with Team ASIJ_Tokyo, Team Korea_HS hosted the 2021 Autumn Dry Lab Webinar providing a walkthrough of mainstream dry lab programs and activities.
Date : September 30th, 2021
Event : BHA-Jeju Synthetic Biology Workshop
Agenda : Bojeong and Chaehyun held a Synthetic Biology Workshop at BHA-Jeju. They discussed an experimental analysis of our project and performed DNA extractions and gel electrophoresis.
Date : October 3rd, 2021
Event : ASIJ_Tokyo ✕ Korea_HS YouTube Channel
Agenda : Team ASIJ_Tokyo and Korea_HS launched a shared YouTube channel and uploaded two videos (interview compilation and animation) promoting breast cancer awareness.
Date : October 4th, 2021
Event : Lab Kit Webinar
Agenda : Hannah and Matthew hosted virtual lab kit webinars for elementary (“Extracting DNA from Bananas”) and middle (“Designing Your Own Petri Dish”) school students.
Date : October 6th, 2021
Event : SAS-Puxi Synthetic Biology Journalism
Agenda : Haryeong submitted a proposal to design a cancer-specific cell-penetrating peptide for the efficient delivery of siRNA into cancer cells to International Youth Scientists in SAS-Puxi.
Date : October 16th, 2021
Event : “Give a Blood~Save a Life”
Agenda : Under the partnership with Team Greece_United, Team Korea_HS held a group blood donation event at Gangnam, Seoul, educating the public on the necessity of safe blood donations.
Date : October 17th, 2021
Event : “How To iGEM” Booklet
Agenda : Under the partnership with Team Greece_United, Team Korea_HS co-authored the “How To iGEM” booklet, an engaging guideline to all aspiring iGEMers.
Team Inclusion
Date : August 15th, 2021
Event : First Inclusion meeting
Agenda : Introduced sub-group, project decisions, role division across projects, and deadline establishment to the team members.
Date : September 9th, 2021
Event : First themed posting on Korea_HS Instagram account by Bojeong
Agenda : Posted about Alan Hart, an American radiologist, in order to increase awareness about LGBTQ+ STEM pioneers.
Date : September 22nd, 2021
Event : Finalization of LGBTQ+ in STEM inclusion report by Hannah and Ellie
Agenda : Completed all parts of Inclusion report: Introduction, Analysis of Status-Quo, Significance, Plan of Action, and Conclusion.
Date : October 18th, 2021
Event : LGBTQ+ STEM webinar through zoom event hosted by Matthew and Hannah
Agenda : Discussed issues specific to LGBTQ+ in STEM, the significance of ameliorating such issues, a plan of action to target the issues, and a short conclusion and takeaways.
Date : October 20th, 2021
Event : Interview with LGBTQ+ affiliated STEM personnel Professor Ramirez
Agenda : Discussion of STEM LGBTQ+ in the status-quo and how to ameliorate issues faced by LGBTQ+ in STEM.
Korea_HS-Hosted Events
Date : August 8th, 2021
Event : Korea Meetup
Agenda : The team convened with Korean iGEMers – KUAS_Korea and Yonsei_KOR – sharing project ideas and discussing possibilities of local collaboration (education) initiatives.
Date : September 24th, 2021
Event : Northeast Asia Joint Collaboration Network
Agenda : The team convened with high school teams of Northeast Asia (Japan, China, Taiwan, etc) and discussed opportunities for a common communication platform for complementary support.
Date : September 25th, 2021
Event : 1st Global iGEM Diagnostics & Therapeutics Conference
Agenda : The team hosted the 1st Global iGEM Diagnostics & Therapeutics Conference to discuss the role of Synthetic Biology in future diagnostics and therapeutics with five other teams.
Date : October 7th, 2021
Event : Instagram Project
Agenda : The team created the Instagram Project. It was a collaborative outreach initiative connecting global iGEMers via an individualized posting (with contact and project information) for each team.