Attributions
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Team member
Each member's attributions are listed in the table below.
Kento Tamukai | Yuki Kobayashi | Alexander Liu | Chisato Nisizawa | Momoko Hayashida | Nao Tajima | Yutaro Ikeda | Chihiro Goya | Hibiki Tamura | Hiroto Koga | Kaho Tanaka | Kodai Shiotu | Rikuto Fukushima | Ryotaro Hirota | Saki Kawakami | Taiyo Hayashi | Yuto Ueda | Ayumi Morita | Kaho Maeta | Kazuyoshi Nakaji | Ryusei Hayamatsu | ||||
Wet Lab | Biomolecule Production Platform | APP | plasmid design | ✓✓ | ✓ | ✓✓ | ||||||||||||||||||
plasmid construction | ✓✓ | ✓✓ | ✓✓ | ✓✓ | ||||||||||||||||||||
ssrA tag mutagenesis | ✓✓ | |||||||||||||||||||||||
Infectious Disease Detection | gRNA & primer design | ✓✓ | ✓ | |||||||||||||||||||||
Cas12a-mitigated LAMP/RT-LAMP assay | ✓✓ | |||||||||||||||||||||||
Killing Pests | plasmid design | ✓✓ | ||||||||||||||||||||||
plasmid construction | ✓✓ | |||||||||||||||||||||||
dsRNA preperation | ✓✓ | |||||||||||||||||||||||
mortality assay | ✓✓ | ✓✓ | ||||||||||||||||||||||
Anti-Aging reagent | plasmid design | ✓✓ | ✓✓ | |||||||||||||||||||||
plasmid construction | ✓ | ✓✓ | ||||||||||||||||||||||
dsRNA preperation | ✓ | ✓✓ | ||||||||||||||||||||||
flower wilting assay | ✓✓ | ✓ | ✓ | |||||||||||||||||||||
whitening rate assay | ✓✓ | ✓ | ✓ | |||||||||||||||||||||
peptide preperation | ✓✓ | ✓✓ | ||||||||||||||||||||||
Anti-Bacterial system | plasmid design | ✓✓ | ✓✓ | |||||||||||||||||||||
plasmid construction | ✓✓ | |||||||||||||||||||||||
peptide preperation | ✓✓ | ✓✓ | ✓✓ | |||||||||||||||||||||
anti-bacterial assay | ✓✓ | ✓ | ||||||||||||||||||||||
Bacterial flora analysis | ✓✓ | ✓✓ | ✓ | |||||||||||||||||||||
Dry Lab | Modeling | ✓ | ✓✓ | ✓✓ | ✓ | |||||||||||||||||||
Software | ✓✓ | |||||||||||||||||||||||
Hardware | ✓ | ✓ | ✓ | |||||||||||||||||||||
NGS analysis | ✓ | ✓✓ | ||||||||||||||||||||||
Public relations | Human Practices | ✓✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓✓ | ||||||||||||||
Partnership | ✓✓ | |||||||||||||||||||||||
Collaboration | ✓✓ | |||||||||||||||||||||||
Education | ✓✓ | ✓✓ | ✓✓ | |||||||||||||||||||||
Sponsorship | ✓✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | |||||||||||||||||
SDGs | ✓✓ | ✓✓ | ✓✓ | |||||||||||||||||||||
Excellence in another area | ✓✓ | ✓ | ✓✓ | |||||||||||||||||||||
Submissions | Presentation video | direction | ✓✓ | ✓✓ | ||||||||||||||||||||
editing | ✓✓ | |||||||||||||||||||||||
Project promotion video | direction | ✓✓ | ✓✓ | |||||||||||||||||||||
editing | ✓✓ | |||||||||||||||||||||||
appearance | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | |||||||||||||||
Wiki | writing | ✓ | ✓ | ✓ | ✓✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ||||||
design | ✓✓ | |||||||||||||||||||||||
Graphics | ✓✓ | ✓✓ | ✓✓ | ✓✓ | ✓✓ | |||||||||||||||||||
Accounting | ✓✓ |
Team instructor support
Dr. Makoto Kitabatake
Institute for Frontier Life and Medical Sciences, Kyoto University
He helped us with the whole project. He gave us expert advice, including experimental training, and taught us about team management and safety issues. He also supported us a lot to make our wiki and presentation video better. Moreover, he allowed us to conduct experiments in his laboratory.
He helped us with the whole project. He gave us expert advice, including experimental training, and taught us about team management and safety issues. He also supported us a lot to make our wiki and presentation video better. Moreover, he allowed us to conduct experiments in his laboratory.
Dr. Knut Woltjen
Center for iPS Cell Research and Application, Kyoto University
He helped us with the whole project. He provided expert advice in planning the project. He checked most of our script and supported our wiki and presentation video become better. Moreover, he allowed us to conduct experiments in his laboratory.
He helped us with the whole project. He provided expert advice in planning the project. He checked most of our script and supported our wiki and presentation video become better. Moreover, he allowed us to conduct experiments in his laboratory.
Team advisor
Mr. Masaaki Shimazoe
Faculty of Science, Kyoto University
He helped us with everything related to our project. He supervised the project from start to finish. He took the lead in editing the wiki. Without his help, we would not have been able to complete the project as a whole. In addition, he provided us with E. coli HT115 (DE3) to make dsRNA.
He helped us with everything related to our project. He supervised the project from start to finish. He took the lead in editing the wiki. Without his help, we would not have been able to complete the project as a whole. In addition, he provided us with E. coli HT115 (DE3) to make dsRNA.
Technical support
Dr. Tomoo Shimada
Faculty of Science, Kyoto University
They provided us with a plant growing room and a laboratory. They also provided us with Arabidopsis thaliana and helped us grow it. In addition, they gave us a lot of expert advice on the whole project.
Dr. Ichiro Taniguchi
Institute for Frontier Life and Medical Sciences, Kyoto University
He allowed us to conduct experiments in his laboratory. We deeply appreciated him for accepting that many students come to the lab everyday. He also transported large planters full of flowers for us.
He allowed us to conduct experiments in his laboratory. We deeply appreciated him for accepting that many students come to the lab everyday. He also transported large planters full of flowers for us.
Dr. Hiroko Watanabe
Dr. Michiki Takeuchi
Dr. Jun Ogawa
Laboratory for Circular Bioeconomy Development, Office of Society Academia Collaboration for Innovation, Kyoto University
Industrial Microbiology, Graduate School of Agriculture, Kyoto University
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
We were allowed to conduct experiments in their laboratory. They trained us on the experiment and supervised us to conduct experiments safely. Moreover, they had many meetings with us and advised us on the whole project. In particular, they gave a lot of advice on the experiments of antimicrobial peptides and biofilm degradation enzymes. They also gave us their experts’ comments on our Biomolecular Production Platform: BLOOM.
Mr. Ryo Niwa
Center for iPS Cell Research and Application, Kyoto University
NGS data were processed and analyzed by him. We had the opportunity to have several meetings with him and received advice on NGS analysis.
NGS data were processed and analyzed by him. We had the opportunity to have several meetings with him and received advice on NGS analysis.
Ms. Kako Nakazato
Graduate School of Agriculture, Kyoto University
She helped us to create the illustrations for wiki and our presentation video. Thanks to her beautiful illustrations, the quality of the wiki and the video has been really improved. We really appreciated her.
She helped us to create the illustrations for wiki and our presentation video. Thanks to her beautiful illustrations, the quality of the wiki and the video has been really improved. We really appreciated her.
Mr. Ren Takimoto
Faculty of Agriculture, Kyoto University
He helped us in editting our wiki. He corrected a large number of English sentences in a short period of time. We really appreciated him.
He helped us in editting our wiki. He corrected a large number of English sentences in a short period of time. We really appreciated him.
Dr. Shiro Futaki
Professor of Biochemistry, Institute for Chemical Research, Kyoto University
He allowed us to synthesize NOP-1 peptide in his lab and also suggested an experimental method.
He allowed us to synthesize NOP-1 peptide in his lab and also suggested an experimental method.
Dr. Shunnsuke Asano
Nara Prefecture Agricultural Research and Development Center
We had a chance to have an online meeting with him and discussed virus detection. We also received RNA samples of Dahlia Mosaic Virus(DMV), Chrysanthemum Stunt Viroid(CSVd), and Tomato Spotted Wilt Virus(TSWV).
Dr. Susumu Tokumaru
Kyoto Prefecture Agriculture Forestry and Fisheries Technology Center
He bred and provided us with western flower thrips. He also gave us advice on how to conduct experiments and showed us how to operate them.
Dr. Takeshi Nakano
Faculty of Agriculture, Kyoto Universityr
We had several meetings with him and he advised us about the whole project. We also received Brassinolide and a brassinosteroid biosynthesis inhibitor (Brz).
Dr. Wataru Aoki
Faculty of Agriculture, Kyoto University
We had many meetings with him and he advised us on the design of the biomolecular production platform: BLOOM. He also provided us with reagents for Tricin SDS-PAGE, which was used to determine the purity of low-molecular-weight peptides.
We had many meetings with him and he advised us on the design of the biomolecular production platform: BLOOM. He also provided us with reagents for Tricin SDS-PAGE, which was used to determine the purity of low-molecular-weight peptides.
Mr. Yuishinn Kosaka
Faculty of Agriculture, Kyoto University
He taught us the protocol for Tricin SDS-PAGE. He also helped us to prepare the reagents.
He taught us the protocol for Tricin SDS-PAGE. He also helped us to prepare the reagents.
Ms. Yukiko Nakahara
Office of Society-Academia Collaboration for Innovation
She offered us the space of Kyoto University Entrepreneur Platform. Our Wiki writing was not possible without her generous offer.
She offered us the space of Kyoto University Entrepreneur Platform. Our Wiki writing was not possible without her generous offer.
Project advisor support
Dr. Atsushi Ishida
Center for Ecological Research, Kyoto University
He taught us about the effects of cavitation on plants and the ability of plants to eliminate cavitation.
He taught us about the effects of cavitation on plants and the ability of plants to eliminate cavitation.
Dr. Kenichi Shibuya
National Agriculture and Food Research Organization
We learned about the aging mechanism of flowers and efforts to make flowers last longer.
We learned about the aging mechanism of flowers and efforts to make flowers last longer.
Dr. Naoki Honda
Graduate School of Integrated Sciences for Life, Hiroshima University
He advised us on how to model asymmetric plasmid partitioning.
Dr. Nobuhiko Nomura
University of Tsukuba Life and Environmental Sciences
We received some inspirations on the relationship between plants and bacterial biofilms.
We received some inspirations on the relationship between plants and bacterial biofilms.
Dr. Satoshi Ohara
Institute for Future Initiatives, The University of Tokyo
We received advice on how to implement the Life Cycle Assessment (LCA). He also allowed us to watch his lecture video about LCA.
Dr. Shirou Nakao
Faculty of Life and Environmental Sciences, Kyoto Prefectural University
We had the opportunity to have an online meeting with him. He taught us how to grow and operate thrips and how important RNAi research against pests are.
Dr. Syouji Sonoda
Faculty of Agriculture, Utsunomiya University
We had the opportunity to have an online meeting with him. We learned how to grow and operate thrips from him.
We had the opportunity to have an online meeting with him. We learned how to grow and operate thrips from him.
Dr. Takehisa Yano
Kao Corporation
We had the opportunity to have an online meeting with him. Thanks to him, we realized how interesting biofilm control is.
Prof. Dr. Takeshi Miki
Faculty of Advanced Science and Technology, Ryukoku University
We had an online meeting and he advised us on how to model asymmetric plasmid partitioning.
Dr. Tomohiro Nobeyama
University of Tsukuba Science and Technology
We had an online meeting with him. He gave us advice on how to model asymmetric plasmid partitioning.
Human practice support
Mr. Takashi Kurokawa
Kurokawa Floriculture
He gave us several opportunities to visit his fields. We learned about the causes of losses during flowering plant growth from him. We also received feedback on our detection system from a farmer's perspective.
Mr. Yoshiyuki Ozawa
KYOTO SEIKA Inc.
He gave us an opportunity to visit the market and learn about the causes of flower loss at the market.
Mr. Masahiro Inoue and all the employees we intervied
Gin Ka-En
We had several opportunities to talk with the store owners and learn about the causes of flower disposal and the countermeasures. We also asked their opinions on the use of antimicrobial peptides. Moreover, they provided us with water samples to be used in our experiments.
We had several opportunities to talk with the store owners and learn about the causes of flower disposal and the countermeasures. We also asked their opinions on the use of antimicrobial peptides. Moreover, they provided us with water samples to be used in our experiments.
Mr. Chikara Ishidou
Representative Director and CEO, Hanane CO.
He gave us the opportunity to have an online meeting with him and learn about the current state of the flower industry in Japan and why flower loss occurs.
HANACHO Florist
Shogoin Store
From their perspective as flower sellers, they taught us how important it is to pay attention to the quality of the flowers we sell.
From their perspective as flower sellers, they taught us how important it is to pay attention to the quality of the flowers we sell.
Mr. Takurou Ishihara
SUNTORY FLOWERS
We had the opportunity to have an online meeting with him. We learned about the current state of the floral industry from a global perspective and received feedback on the project as a whole.
Mr. Hiroaki Fujii
Kyoto Vegetables Promotion Section, Agricultural Industry Division, Department of Agriculture, Forestry and Fisheries, Kyoto Prefecture
We were given the opportunity to visit them in person and get their opinions on our virus detection project from a practical point of view. They also helped us to conduct a questionnaire to get the farmers' opinions on the practical use of our virus detection project.
We were given the opportunity to visit them in person and get their opinions on our virus detection project from a practical point of view. They also helped us to conduct a questionnaire to get the farmers' opinions on the practical use of our virus detection project.
Mr. Yoshinori Shimazu
IKENOBO
They gave us the opportunity to visit their practice and learn about the history and spirit of ikebana. They also invited us to their flower arrangement class and helped us photograph their flower arrangement works.
Education & Communications
Mr. Yuichiro Shin
Sci-tech promotion committee of Hyogo Prefecture・Teacher, Hyogo Prefectural Kakogawa Higashi High School
He gave us the opportunity to give an online lecture on synthetic biology to high school students.
He gave us the opportunity to give an online lecture on synthetic biology to high school students.
Excellence in another area
Dr.Shunichi Kashida
Dr. Kaoru Richard Komatsu
Mr. Kenn Tajiri
Ms. Sayaka Dantsuji
xFOREST Therapeutics
xFOREST Therapeutics
xFOREST Therapeutics
xFOREST Therapeutics
They gave us the opportunity to have an interview for the article “Gifts from Synthetic Biology”.
Wiki edit support
iGEM BITSPilani-Goa India 2020
We used a wiki editing tool called iGEM wikisync, developed by the iGEM BITSPilani-Goa India 2020 team.
Collaboration & Partnership
iGEM Gunma
The purified biofilm-degrading enzymes were provided by them. We were introduced to Mr. Shimazu
iGEM UTokyo
They provided us with yeast supernatant containing defensin-3.
iGEM NCTU-Formosa
They had an online meeting and exchanged information with us.
iGEM NTU-Singapore
They had an online meeting and exchanged information with us.
Sponsors
TOYOBO CO., LTD.
They provided us with financial support and reagents.
NAGASE & CO., LTD.
They provided us with financial support.
Cosmo Bio CO., LTD.
They provided us with financial support.
NIPPON Genetics
They provided us with financial support.
Beckman Coulter, Inc.
They provided us with reagents.
Promega Corporation.
They provided us with reagents.
BioRender
They allowed us to use their tools.
Integrated DNA Technologies, Inc.
They provided us with reagents.
Twist Bioscience
They provided us with reagents.