Team:Kyoto/Parts
Parts
Basic Part
Overview
Our best basic part is BBa_K3815000.
This is composed of
ELK16, PT linker, Mxe GyrA intein. It can be expressed by attaching the sequence of the
targeted protein to the N-terminus of Intein. This part is used for the purification method
called ELK16system, which past iGEM teams had not been used. In this ELK16system, the ELK16
part is self-condensing, so the fusion protein can be extracted only by centrifugation, and
the intein part is self-cleaved by DTT, so the target protein can be easily separated. The
ELK16system will help improve the technology of recombinant protein purification, although
it has some problems such as the presence of many other impurities in the protein recovery
process.
Fig1. The mechanism of ELK16 system
Basic Part List
| Name | Type | Part Name | Diagram | Designer |
|---|---|---|---|---|
| BBa_K3815000 | Protein Coding | ELK16-PT Linker-Mxe GyrA intein |
|
Koudai Shiotsu |
| BBa_K3815001 | Protein Coding | ELK16-PT Linker-Mxe GyrA intein-CecropinA |
|
Koudai Shiotsu |
| BBa_K3815002 | Protein Coding | ELK16-PT Linker-Mxe GyrA intein-Defensin1 |
|
Koudai Shiotsu |
| BBa_K3815003 | Protein Coding | ELK16-PT Linker-Mxe GyrA intein-LL37 |
|
Koudai Shiotsu |
| BBa_K3815004 | Protein Coding | ELK16-PT Linker-Mxe GyrA intein-NOP1 |
|
Koudai Shiotsu |
| BBa_K3815005 | Protein Coding | ELK16-PT Linker-Mxe GyrA intein-NOP1v |
|
Koudai Shiotsu |
| BBa_K3815006 | Protein Coding | His tag-PT Linker-Mxe GyrA intein-CecropinA |  |
Hiroto Koga |
| BBa_K3815007 | Protein Coding | His tag-PT Linker-Mxe GyrA intein-Defensin1 |
|
Hiroto Koga |
| BBa_K3815008 | Protein Coding | His tag-PT Linker-Mxe GyrA intein-LL37 |
|
Hiroto Koga |
| BBa_K3815009 | Protein Coding | His tag-PT Linker-Mxe GyrA intein-NOP1 |
|
Hiroto Koga |
| BBa_K3815010 | Protein Coding | His tag-PT Linker-Mxe GyrA intein-NOP1v |
|
Hiroto Koga |
| BBa_K3815011 | RNA | V-ATPaseB | N/A | Yuto Ueda |
| BBa_K3815012 | RNA | TrxZ -3 | N/A | Yuto Ueda |
| BBa_K3815013 | RNA | EPH1 | N/A | Yuto Ueda |
| BBa_K3815032 | Protein Coding | B. stearothermophilus DNA polymerase | N/A | Paper |
| BBa_K3815033 | Protein Coding | HIV-1 reverse transcriptase p51 | N/A | Paper |
| BBa_K3815034 | Protein Coding | HIV-1 reverse transcriptase p66 | N/A | Paper |
Improvement of an Existing Part
Overview
Each of the parts listed below are the improvement from BBa_K1051206, the wildtype ssrA tag.
Three C-terminal amino acids LAA in WT tag are replaced in these mutants, therefore
resulting in a reduced protein degradation efficiency to various degrees. The collection of
these mutants consist of a large repertoire of protein degradation tags of various levels,
which can be applied for fine-tuning of many kinds of synthetic systems that require a
precise control of protein half-life (see ssrA results in the Results page).
Improvement of an Existing Part List
| Name | Type | Part Name | Designer |
|---|---|---|---|
| BBa_K3815015 | Tag | AANDENYALGA.mutant SsrA degradation tag | Alexander Liu |
| BBa_K3815016 | Tag | AANDENYAKWA.mutant SsrA degradation tag | Alexander Liu |
| BBa_K3815017 | Tag | AANDENYALAK.mutant SsrA degradation tag | Alexander Liu |
| BBa_K3815018 | Tag | AANDENYAHPK.mutant SsrA degradation tag | Alexander Liu |
| BBa_K3815019 | Tag | AANDENYALAL.mutant SsrA degradation tag | Alexander Liu |
| BBa_K3815020 | Tag | AANDENYANDK.mutant SsrA degradation tag | Alexander Liu |
| BBa_K3815021 | Tag | AANDENYANEN.mutant SsrA degradation tag | Alexander Liu |
| BBa_K3815022 | Tag | AANDENYAYES.mutant SsrA degradation tag | Alexander Liu |
| BBa_K3815023 | Tag | AANDENYAHAK.mutant SsrA degradation tag | Alexander Liu |
| BBa_K3815024 | Tag | AANDENYALAS.mutant SsrA degradation tag | Alexander Liu |
| BBa_K3815025 | Tag | AANDENYATAP.mutant SsrA degradation tag | Alexander Liu |
| BBa_K3815026 | Tag | AANDENYANAA.mutant SsrA degradation tag | Alexander Liu |
| BBa_K3815027 | Tag | AANDENYAKLA.mutant SsrA degradation tag | Alexander Liu |
| BBa_K3815028 | Tag | AANDENYALPA.mutant SsrA degradation tag | Alexander Liu |
| BBa_K3815029 | Tag | AANDENYALAM.mutant SsrA degradation tag | Alexander Liu |
| BBa_K3815030 | Tag | AANDENYALAI.mutant SsrA degradation tag | Alexander Liu |
| BBa_K3815031 | Tag | AANDENYAPHA.mutant SsrA degradation tag | Alexander Liu |