Team:Kyoto/Parts

Parts

Our best basic part is BBa_K3815000. This is composed of ELK16, PT linker, Mxe Gyr intein. It can be expressed by attaching the sequence of the targeted protein to the N-terminus of Intein. This part is used for the purification method called ELK16system, which past iGEM teams had not been used. In this ELK16system, the ELK16 part is self-condensing, so the fusion protein can be extracted only by centrifugation, and the intein part is self-cleaved by DTT, so the target protein can be easily separated. The ELK16system will help improve the technology of recombinant protein purification, although it has some problems such as the presence of many other impurities in the protein recovery process.

Basic Part List

Name	Type	Part Name	Designer
BBa_K3815000	Protein Coding	ELK16-PT Linker-Mxe Gyr intein	Koudai Shiotsu
BBa_K3815001	Protein Coding	ELK16-PT Linker-Mxe Gyr intein-CecropinA	Koudai Shiotsu
BBa_K3815002	Protein Coding	ELK16-PT Linker-Mxe Gyr intein-Defensin1	Koudai Shiotsu
BBa_K3815003	Protein Coding	ELK16-PT Linker-Mxe Gyr intein-LL37	Koudai Shiotsu
BBa_K3815004	Protein Coding	ELK16-PT Linker-Mxe Gyr intein-NOP1	Koudai Shiotsu
BBa_K3815005	Protein Coding	ELK16-PT Linker-Mxe Gyr intein-NOP1v	Koudai Shiotsu
BBa_K3815006	Protein Coding	His tag-PT Linker-Mxe Gyr intein-CecropinA	Hiroto Koga
BBa_K3815007	Protein Coding	His tag-PT Linker-Mxe Gyr intein-Defensin1	Hiroto Koga
BBa_K3815008	Protein Coding	His tag-PT Linker-Mxe Gyr intein-LL37	Hiroto Koga
BBa_K3815009	Protein Coding	His tag-PT Linker-Mxe Gyr intein-NOP1	Hiroto Koga
BBa_K3815010	Protein Coding	His tag-PT Linker-Mxe Gyr intein-NOP1v	Hiroto Koga
BBa_K3815011	RNA	V-ATPaseB	Yuto Ueda
BBa_K3815012	RNA	TrxZ -3	Yuto Ueda
BBa_K3815013	RNA	EPH1	Yuto Ueda
BBa_K3815014	Composite	iRFP 670-superfolder GFP	Yuto Ueda
BBa_K3815015	Tag	AANDENYALGA.mutant SsrA degradation tag	Alexander Liu
BBa_K3815016	Tag	KWADENYALGA.mutant SsrA degradation tag	Alexander Liu
BBa_K3815017	Tag	LAKDENYALGA.mutant SsrA degradation tag	Alexander Liu
BBa_K3815018	Tag	HPKDENYALGA.mutant SsrA degradation tag	Alexander Liu
BBa_K3815019	Tag	LALDENYALGA.mutant SsrA degradation tag	Alexander Liu
BBa_K3815020	Tag	NDKDENYALGA.mutant SsrA degradation tag	Alexander Liu
BBa_K3815021	Tag	NENDENYALGA.mutant SsrA degradation tag	Alexander Liu
BBa_K3815022	Tag	YESDENYALGA.mutant SsrA degradation tag	Alexander Liu
BBa_K3815023	Tag	HAKDENYALGA.mutant SsrA degradation tag	Alexander Liu
BBa_K3815024	Tag	LASDENYALGA.mutant SsrA degradation tag	Alexander Liu
BBa_K3815025	Tag	TAPDENYALGA.mutant SsrA degradation tag	Alexander Liu
BBa_K3815026	Tag	NAADENYALGA.mutant SsrA degradation tag	Alexander Liu
BBa_K3815027	Tag	KLADENYALGA.mutant SsrA degradation tag	Alexander Liu
BBa_K3815028	Tag	LPADENYALGA.mutant SsrA degradation tag	Alexander Liu
BBa_K3815029	Tag	LAMDENYALGA.mutant SsrA degradation tag	Alexander Liu
BBa_K3815030	Tag	LAIDENYALGA.mutant SsrA degradation tag	Alexander Liu
BBa_K3815031	Tag	PHADENYALGA.mutant SsrA degradation tag	Alexander Liu
BBa_K3815032	Tag	B. stearothermophilus DNA polymerase	Paper
BBa_K3815033	Tag	HIV-1 reverse transcriptase p51	Paper
BBa_K3815034	Tag	HIV-1 reverse transcriptase p66	Paper

Fig1. The mechanism of ELK16 system