Difference between revisions of "Team:Kyoto/Parts"

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<div id="sctarget1-1" class="myh2">Basic Part List</div>
 
 
  <div class="myp">Our best basic part is <a href="http://parts.igem.org/Part:BBa_K3815000">BBa_K3815000</a>. This is composed of ELK16, PT linker, Mxe Gyr intein. It can be expressed by attaching the sequence of the targeted protein to the N-terminus of Intein. This part is used for the purification method called ELK16system, which past iGEM teams had not been used. In this ELK16system, the ELK16 part is self-condensing, so the fusion protein can be extracted only by centrifugation, and the intein part is self-cleaved by DTT, so the target protein can be easily separated. The ELK16system will help improve the technology of recombinant protein purification, although it has some problems such as the presence of many other impurities in the protein recovery process. </div>
 
  <div class="myp">Our best basic part is <a href="http://parts.igem.org/Part:BBa_K3815000">BBa_K3815000</a>. This is composed of ELK16, PT linker, Mxe Gyr intein. It can be expressed by attaching the sequence of the targeted protein to the N-terminus of Intein. This part is used for the purification method called ELK16system, which past iGEM teams had not been used. In this ELK16system, the ELK16 part is self-condensing, so the fusion protein can be extracted only by centrifugation, and the intein part is self-cleaved by DTT, so the target protein can be easily separated. The ELK16system will help improve the technology of recombinant protein purification, although it has some problems such as the presence of many other impurities in the protein recovery process. </div>
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<table border="1" width="80%" style="center">
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<div id="sctarget1-1" class="myh2">Basic Part List</div>
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<table border="1" width="100%">
 
   <tr>
 
   <tr>
 
       <th>Name</th>
 
       <th>Name</th>
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       <td>Protein Coding</td>
 
       <td>Protein Coding</td>
 
       <td>ELK16-PT Linker-Mxe Gyr intein</td>
 
       <td>ELK16-PT Linker-Mxe Gyr intein</td>
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       <td><div class ="figs">
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       <td>
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      <div class ="figs">
 
         <img class="h70" src="https://static.igem.org/mediawiki/2021/c/cc/T--kyoto--plasmid_ELK16.png">
 
         <img class="h70" src="https://static.igem.org/mediawiki/2021/c/cc/T--kyoto--plasmid_ELK16.png">
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    </div></td>
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      </div></td>
 
       <td>Koudai Shiotsu</td>
 
       <td>Koudai Shiotsu</td>
 
     </tr>
 
     </tr>
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       <td>ELK16-PT Linker-Mxe Gyr intein-CecropinA</td>
 
       <td>ELK16-PT Linker-Mxe Gyr intein-CecropinA</td>
 
       <td>
 
       <td>
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    <div class ="figs">
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      <div class ="figs">
 
         <img class="h70" src="https://static.igem.org/mediawiki/2021/c/c0/T--kyoto--CecA-ELK16.png">
 
         <img class="h70" src="https://static.igem.org/mediawiki/2021/c/c0/T--kyoto--CecA-ELK16.png">
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    </div>
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      </div>
 
       </td>
 
       </td>
 
       <td>Koudai Shiotsu</td>
 
       <td>Koudai Shiotsu</td>
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       <td>Protein Coding</td>
 
       <td>Protein Coding</td>
 
       <td>ELK16-PT Linker-Mxe Gyr intein-Defensin1</td>
 
       <td>ELK16-PT Linker-Mxe Gyr intein-Defensin1</td>
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       <td><div class ="figs">
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       <td>
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      <div class ="figs">
 
         <img class="h70" src="https://static.igem.org/mediawiki/2021/8/89/T--kyoto--Def1_ELK16.png">
 
         <img class="h70" src="https://static.igem.org/mediawiki/2021/8/89/T--kyoto--Def1_ELK16.png">
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    </div></td>
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      </div></td>
 
       <td>Koudai Shiotsu</td>
 
       <td>Koudai Shiotsu</td>
 
     </tr>
 
     </tr>
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       <td>Protein Coding</td>
 
       <td>Protein Coding</td>
 
       <td>ELK16-PT Linker-Mxe Gyr intein-LL37</td>
 
       <td>ELK16-PT Linker-Mxe Gyr intein-LL37</td>
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       <td><div class ="figs">
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       <td>
 +
      <div class ="figs">
 
         <img class="h70" src="https://static.igem.org/mediawiki/2021/e/e5/T--kyoto--LL37_ELK16.png">
 
         <img class="h70" src="https://static.igem.org/mediawiki/2021/e/e5/T--kyoto--LL37_ELK16.png">
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    </div></td>
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      </div></td>
 
       <td>Koudai Shiotsu</td>
 
       <td>Koudai Shiotsu</td>
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    </tr>
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    </tr>
 
       <td><a href="http://parts.igem.org/Part:BBa_K3815004">BBa_K3815004</a></td>
 
       <td><a href="http://parts.igem.org/Part:BBa_K3815004">BBa_K3815004</a></td>
 
       <td>Protein Coding</td>
 
       <td>Protein Coding</td>
 
       <td>ELK16-PT Linker-Mxe Gyr intein-NOP1</td>
 
       <td>ELK16-PT Linker-Mxe Gyr intein-NOP1</td>
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       <td height="30"><div class ="figs">
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       <td height="30">
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      <div class ="figs">
 
         <img class="h70" src="https://static.igem.org/mediawiki/2021/3/3f/T--kyoto--NOP1_ELK16.png">
 
         <img class="h70" src="https://static.igem.org/mediawiki/2021/3/3f/T--kyoto--NOP1_ELK16.png">
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    </div></td>
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      </div></td>
 
       <td>Koudai Shiotsu</td>
 
       <td>Koudai Shiotsu</td>
 
     </tr>
 
     </tr>

Revision as of 16:28, 21 October 2021

Parts
- Click to visit -
Our best basic part is BBa_K3815000. This is composed of ELK16, PT linker, Mxe Gyr intein. It can be expressed by attaching the sequence of the targeted protein to the N-terminus of Intein. This part is used for the purification method called ELK16system, which past iGEM teams had not been used. In this ELK16system, the ELK16 part is self-condensing, so the fusion protein can be extracted only by centrifugation, and the intein part is self-cleaved by DTT, so the target protein can be easily separated. The ELK16system will help improve the technology of recombinant protein purification, although it has some problems such as the presence of many other impurities in the protein recovery process.
Basic Part List
Name Type Part Name Diagram Designer
BBa_K3815000 Protein Coding ELK16-PT Linker-Mxe Gyr intein
Koudai Shiotsu
BBa_K3815001 Protein Coding ELK16-PT Linker-Mxe Gyr intein-CecropinA
Koudai Shiotsu
BBa_K3815002 Protein Coding ELK16-PT Linker-Mxe Gyr intein-Defensin1
Koudai Shiotsu
BBa_K3815003 Protein Coding ELK16-PT Linker-Mxe Gyr intein-LL37
Koudai Shiotsu
BBa_K3815004 Protein Coding ELK16-PT Linker-Mxe Gyr intein-NOP1
Koudai Shiotsu
BBa_K3815005 Protein Coding ELK16-PT Linker-Mxe Gyr intein-NOP1v
Koudai Shiotsu
BBa_K3815006 Protein Coding His tag-PT Linker-Mxe Gyr intein-CecropinA Hiroto Koga
BBa_K3815007 Protein Coding His tag-PT Linker-Mxe Gyr intein-Defensin1
Hiroto Koga
BBa_K3815008 Protein Coding His tag-PT Linker-Mxe Gyr intein-LL37
Hiroto Koga
BBa_K3815009 Protein Coding His tag-PT Linker-Mxe Gyr intein-NOP1
Hiroto Koga
BBa_K3815010 Protein Coding His tag-PT Linker-Mxe Gyr intein-NOP1v
Hiroto Koga
BBa_K3815011 RNA V-ATPaseB Yuto Ueda
BBa_K3815012 RNA TrxZ -3 Yuto Ueda
BBa_K3815013 RNA EPH1 Yuto Ueda
BBa_K3815014 Composite iRFP 670-superfolder GFP Yuto Ueda
BBa_K3815015 Tag AANDENYALGA.mutant SsrA degradation tag Alexander Liu
BBa_K3815016 Tag KWADENYALGA.mutant SsrA degradation tag Alexander Liu
BBa_K3815017 Tag LAKDENYALGA.mutant SsrA degradation tag Alexander Liu
BBa_K3815018 Tag HPKDENYALGA.mutant SsrA degradation tag Alexander Liu
BBa_K3815019 Tag LALDENYALGA.mutant SsrA degradation tag Alexander Liu
BBa_K3815020 Tag NDKDENYALGA.mutant SsrA degradation tag Alexander Liu
BBa_K3815021 Tag NENDENYALGA.mutant SsrA degradation tag Alexander Liu
BBa_K3815022 Tag YESDENYALGA.mutant SsrA degradation tag Alexander Liu
BBa_K3815023 Tag HAKDENYALGA.mutant SsrA degradation tag Alexander Liu
BBa_K3815024 Tag LASDENYALGA.mutant SsrA degradation tag Alexander Liu
BBa_K3815025 Tag TAPDENYALGA.mutant SsrA degradation tag Alexander Liu
BBa_K3815026 Tag NAADENYALGA.mutant SsrA degradation tag Alexander Liu
BBa_K3815027 Tag KLADENYALGA.mutant SsrA degradation tag Alexander Liu
BBa_K3815028 Tag LPADENYALGA.mutant SsrA degradation tag Alexander Liu
BBa_K3815029 Tag LAMDENYALGA.mutant SsrA degradation tag Alexander Liu
BBa_K3815030 Tag LAIDENYALGA.mutant SsrA degradation tag Alexander Liu
BBa_K3815031 Tag PHADENYALGA.mutant SsrA degradation tag Alexander Liu
BBa_K3815032 Tag B. stearothermophilus DNA polymerase Paper
BBa_K3815033 Tag HIV-1 reverse transcriptase p51 Paper
BBa_K3815034 Tag HIV-1 reverse transcriptase p66 Paper
Fig1. The mechanism of ELK16 system
Contact Us
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E-mail: igemkyoto2021[at]gmail.com