Difference between revisions of "Team:GreatBay SCIE/Dissociation constant"

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<div class="title">Optimum liposome aptamer ratio</div>
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<div class="title">Dissociation constant</div>
 
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<p>With the data, we can simply calculate the kd value by using following equation.</p>
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<p>At first we planned to calculate Kd which can be derived from Hill equation</p>
  
 
<p>$$\ce{A + T<-->T[K_{on}][K_{off}]AT}$$</p>
 
<p>$$\ce{A + T<-->T[K_{on}][K_{off}]AT}$$</p>
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<p>$$k_{on}[A][T]=K_{off}[AT]$$</p>
 
<p>$$k_{on}[A][T]=K_{off}[AT]$$</p>
 
<p>$$K_A= \frac{1}{K_D}=\frac{K_{on}}{K_{off}}=\frac{[AT]}{[A][T]}$$</p>
 
<p>$$K_A= \frac{1}{K_D}=\frac{K_{on}}{K_{off}}=\frac{[AT]}{[A][T]}$$</p>
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<p>By using the data, we can compare our affinity with the others' to ensure our binding affinity is outstanding.</p>
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<p>But soon we found that it is impossible to calculate in this way as the seller of the ELISA kit can't tell us the concentration of the aptamer so we had to find another way.</p>
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<p>Besides we used the equation</p>
 
<p>Besides we used the equation</p>
 
<p>$$V=V_{max}\frac{[P]}{K_d\times{[P]}}+M\times[P]$$</p>
 
<p>$$V=V_{max}\frac{[P]}{K_d\times{[P]}}+M\times[P]$$</p>

Revision as of 09:36, 18 October 2021

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Dissociation constant

At first we planned to calculate Kd which can be derived from Hill equation

$$\ce{A + T<-->T[K_{on}][K_{off}]AT}$$

$$k_{on}[A][T]=K_{off}[AT]$$

$$K_A= \frac{1}{K_D}=\frac{K_{on}}{K_{off}}=\frac{[AT]}{[A][T]}$$

But soon we found that it is impossible to calculate in this way as the seller of the ELISA kit can't tell us the concentration of the aptamer so we had to find another way.

Besides we used the equation

$$V=V_{max}\frac{[P]}{K_d\times{[P]}}+M\times[P]$$

As we know the aptamer has the ability to bind with non-specific region.