Difference between revisions of "Team:Kyoto/Parts"
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<li><a href="#sctarget1" class="gr">Basic Part</a></li> | <li><a href="#sctarget1" class="gr">Basic Part</a></li> | ||
| + | <ul> | ||
| + | <li><a href="#sctarget1-1" class="gr">Basic Part List</a></li> | ||
| + | </ul> | ||
<li><a href="#sctarget2" class="gr">Improving Part</a></li> | <li><a href="#sctarget2" class="gr">Improving Part</a></li> | ||
</ul> | </ul> | ||
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<div class="myp center-text-aligner-sidecontent"> | <div class="myp center-text-aligner-sidecontent"> | ||
| − | <div id="sctarget1" class="myh1-green">Basic Part List</div> | + | <div id="sctarget1" class="myh1-green"></div> |
| − | <div class="myp"> | + | <div id="sctarget1-1" class="myh2">Basic Part List</div> |
| + | <div class="myp">Our best basic part is <a href="http://parts.igem.org/Part:BBa_K3815000">BBa_K3815000</a>. This is composed of ELK16, PT linker, Mxe Gyr intein. It can be expressed by attaching the sequence of the targeted protein to the N-terminus of Intein. This part is used for the purification method called ELK16system, which past iGEM teams had not been used. In this ELK16system, the ELK16 part is self-condensing, so the fusion protein can be extracted only by centrifugation, and the intein part is self-cleaved by DTT, so the target protein can be easily separated. The ELK16system will help improve the technology of recombinant protein purification, although it has some problems such as the presence of many other impurities in the protein recovery process. </div> | ||
<table border="1" width="80%" style="center"> | <table border="1" width="80%" style="center"> | ||
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<div class="figs"> | <div class="figs"> | ||
<img class="w40" src="https://static.igem.org/mediawiki/2021/e/e3/T--Kyoto--Engineering_200_ELK16.png"> | <img class="w40" src="https://static.igem.org/mediawiki/2021/e/e3/T--Kyoto--Engineering_200_ELK16.png"> | ||
Revision as of 16:25, 21 October 2021
Parts
Basic Part List
Our best basic part is BBa_K3815000. This is composed of ELK16, PT linker, Mxe Gyr intein. It can be expressed by attaching the sequence of the targeted protein to the N-terminus of Intein. This part is used for the purification method called ELK16system, which past iGEM teams had not been used. In this ELK16system, the ELK16 part is self-condensing, so the fusion protein can be extracted only by centrifugation, and the intein part is self-cleaved by DTT, so the target protein can be easily separated. The ELK16system will help improve the technology of recombinant protein purification, although it has some problems such as the presence of many other impurities in the protein recovery process.
| Name | Type | Part Name | Diagram | Designer |
|---|---|---|---|---|
| BBa_K3815000 | Protein Coding | ELK16-PT Linker-Mxe Gyr intein | 
|
Koudai Shiotsu |
| BBa_K3815001 | Protein Coding | ELK16-PT Linker-Mxe Gyr intein-CecropinA |
|
Koudai Shiotsu |
| BBa_K3815002 | Protein Coding | ELK16-PT Linker-Mxe Gyr intein-Defensin1 | 
|
Koudai Shiotsu |
| BBa_K3815003 | Protein Coding | ELK16-PT Linker-Mxe Gyr intein-LL37 | 
|
Koudai Shiotsu | BBa_K3815004 | Protein Coding | ELK16-PT Linker-Mxe Gyr intein-NOP1 | 
|
Koudai Shiotsu |
| BBa_K3815005 | Protein Coding | ELK16-PT Linker-Mxe Gyr intein-NOP1v | 
|
Koudai Shiotsu |
| BBa_K3815006 | Protein Coding | His tag-PT Linker-Mxe Gyr intein-CecropinA | 
|
Hiroto Koga |
| BBa_K3815007 | Protein Coding | His tag-PT Linker-Mxe Gyr intein-Defensin1 | 
|
Hiroto Koga |
| BBa_K3815008 | Protein Coding | His tag-PT Linker-Mxe Gyr intein-LL37 | 
|
Hiroto Koga |
| BBa_K3815009 | Protein Coding | His tag-PT Linker-Mxe Gyr intein-NOP1 | 
|
Hiroto Koga |
| BBa_K3815010 | Protein Coding | His tag-PT Linker-Mxe Gyr intein-NOP1v | 
|
Hiroto Koga |
| BBa_K3815011 | RNA | V-ATPaseB | Yuto Ueda | |
| BBa_K3815012 | RNA | TrxZ -3 | Yuto Ueda | |
| BBa_K3815013 | RNA | EPH1 | Yuto Ueda | |
| BBa_K3815014 | Composite | iRFP 670-superfolder GFP | Yuto Ueda | |
| BBa_K3815015 | Tag | AANDENYALGA.mutant SsrA degradation tag | Alexander Liu | |
| BBa_K3815016 | Tag | KWADENYALGA.mutant SsrA degradation tag | Alexander Liu | |
| BBa_K3815017 | Tag | LAKDENYALGA.mutant SsrA degradation tag | Alexander Liu | |
| BBa_K3815018 | Tag | HPKDENYALGA.mutant SsrA degradation tag | Alexander Liu | |
| BBa_K3815019 | Tag | LALDENYALGA.mutant SsrA degradation tag | Alexander Liu | |
| BBa_K3815020 | Tag | NDKDENYALGA.mutant SsrA degradation tag | Alexander Liu | |
| BBa_K3815021 | Tag | NENDENYALGA.mutant SsrA degradation tag | Alexander Liu | |
| BBa_K3815022 | Tag | YESDENYALGA.mutant SsrA degradation tag | Alexander Liu | |
| BBa_K3815023 | Tag | HAKDENYALGA.mutant SsrA degradation tag | Alexander Liu | |
| BBa_K3815024 | Tag | LASDENYALGA.mutant SsrA degradation tag | Alexander Liu | |
| BBa_K3815025 | Tag | TAPDENYALGA.mutant SsrA degradation tag | Alexander Liu | |
| BBa_K3815026 | Tag | NAADENYALGA.mutant SsrA degradation tag | Alexander Liu | |
| BBa_K3815027 | Tag | KLADENYALGA.mutant SsrA degradation tag | Alexander Liu | |
| BBa_K3815028 | Tag | LPADENYALGA.mutant SsrA degradation tag | Alexander Liu | |
| BBa_K3815029 | Tag | LAMDENYALGA.mutant SsrA degradation tag | Alexander Liu | |
| BBa_K3815030 | Tag | LAIDENYALGA.mutant SsrA degradation tag | Alexander Liu | |
| BBa_K3815031 | Tag | PHADENYALGA.mutant SsrA degradation tag | Alexander Liu | |
| BBa_K3815032 | Tag | B. stearothermophilus DNA polymerase | Paper | |
| BBa_K3815033 | Tag | HIV-1 reverse transcriptase p51 | Paper | |
| BBa_K3815034 | Tag | HIV-1 reverse transcriptase p66 | Paper |
Fig1. The mechanism of ELK16 system