Difference between revisions of "Team:GreatBay SCIE/Dissociation constant"
| Line 63: | Line 63: | ||
<p>But soon we found that it is impossible to calculate in this way as the seller of the ELISA kit can't tell us the concentration of the aptamer so we had to find another way.</p> | <p>But soon we found that it is impossible to calculate in this way as the seller of the ELISA kit can't tell us the concentration of the aptamer so we had to find another way.</p> | ||
| − | <p> | + | <p>This equation is derived from Michaelis–Menten kinetics</p> |
| − | <p>$$V=V_{max}\frac{[P]}{ | + | <p>$$V=V_{max}\frac{[P]}{K_M\times{[P]}}$$</p> |
| + | <p>With slightly modification, we can obtain the following equation</p> | ||
| + | <p>$$Y=\frac{B_max\times X}{}$$</p> | ||
| + | |||
<p>As we know the aptamer has the ability to bind with non-specific region.</p> | <p>As we know the aptamer has the ability to bind with non-specific region.</p> | ||
</div> | </div> | ||
Revision as of 04:30, 19 October 2021
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Dissociation constant
At first we planned to calculate Kd which can be derived from Hill equation
$$\ce{A + T<-->T[K_{on}][K_{off}]AT}$$
$$k_{on}[A][T]=K_{off}[AT]$$
$$K_A= \frac{1}{K_D}=\frac{K_{on}}{K_{off}}=\frac{[AT]}{[A][T]}$$
But soon we found that it is impossible to calculate in this way as the seller of the ELISA kit can't tell us the concentration of the aptamer so we had to find another way.
This equation is derived from Michaelis–Menten kinetics
$$V=V_{max}\frac{[P]}{K_M\times{[P]}}$$
With slightly modification, we can obtain the following equation
$$Y=\frac{B_max\times X}{}$$
As we know the aptamer has the ability to bind with non-specific region.