Difference between revisions of "Team:Kyoto/Parts"

 
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{{Kyoto}}
 
{{Kyoto}}
 
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    <div class="page-title">Parts</div>
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  <main>
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    <div class="page-top">
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      <div class="page-title">Parts</div>
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    </div>
 
     <div class="sidebar-enabler">
 
     <div class="sidebar-enabler">
 
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           <li><a href="#sctarget1" class="gr">Basic Part</a></li>
 
           <li><a href="#sctarget1" class="gr">Basic Part</a></li>
 
           <ul>
 
           <ul>
−
          <li><a href="#sctarget1-1" class="gr">Basic Part List</a></li>
+
            <li><a href="#sctarget1-x" class="gr">Overview</a></li>
 +
            <li><a href="#sctarget1-1" class="gr">Basic Part List</a></li>
 
           </ul>
 
           </ul>
 
           <li><a href="#sctarget2" class="gr">Improvement of an Existing Part</a></li>
 
           <li><a href="#sctarget2" class="gr">Improvement of an Existing Part</a></li>
 
           <ul>
 
           <ul>
−
          <li><a href="#sctarget2-1" class="gr">Improvement of an Existing Part List</a></li>
+
            <li><a href="#sctarget2-x" class="gr">Overview</a></li>
 +
            <li><a href="#sctarget2-1" class="gr">Improvement of an Existing Part List</a></li>
 
           </ul>
 
           </ul>
 
         </ul>
 
         </ul>
 
       </div>
 
       </div>
  
−
        <div class="maincontents">
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      <div class="maincontents">
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          <div class="myp center-text-aligner-sidecontent">
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        <div class="center-text-aligner-sidecontent">
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<div id="sctarget1" class="myh1-green">Basic Part</div>
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          <div id="sctarget1" class="myh1-green">Basic Part</div>
−
<div class="myp">Our best basic part is <a href="http://parts.igem.org/Part:BBa_K3815000">BBa_K3815000</a>. This is composed of ELK16, PT linker, Mxe Gyr intein. It can be expressed by attaching the sequence of the targeted protein to the N-terminus of Intein. This part is used for the purification method called ELK16system, which past iGEM teams had not been used. In this ELK16system, the ELK16 part is self-condensing, so the fusion protein can be extracted only by centrifugation, and the intein part is self-cleaved by DTT, so the target protein can be easily separated. The ELK16system will help improve the technology of recombinant protein purification, although it has some problems such as the presence of many other impurities in the protein recovery process. </div>
+
          <div id="sctarget1-x" class="myh2">Overview</div>
 +
          <div class="myp">Our best basic part is <a href="http://parts.igem.org/Part:BBa_K3815000">BBa_K3815000</a>.
 +
            This is composed of
 +
            ELK16, PT linker, Mxe GyrA intein. It can be expressed by attaching the sequence of the
 +
            targeted protein to the N-terminus of Intein. This part is used for the purification method
 +
            called ELK16system, which past iGEM teams had not been used. In this ELK16system, the ELK16
 +
            part is self-condensing, so the fusion protein can be extracted only by centrifugation, and
 +
            the intein part is self-cleaved by DTT, so the target protein can be easily separated. The
 +
            ELK16system will help improve the technology of recombinant protein purification, although
 +
            it has some problems such as the presence of many other impurities in the protein recovery
 +
            process. </div>
 +
          <div class="figs">
 +
            <img class="w40" src="https://static.igem.org/mediawiki/2021/e/e3/T--Kyoto--Engineering_200_ELK16.png"
 +
              style="width:500px;">
 +
            <div class="legend">Fig1. The mechanism of ELK16 system</div>
 +
          </div>
  
−
              <center><img class="w40" src="https://static.igem.org/mediawiki/2021/e/e3/T--Kyoto--Engineering_200_ELK16.png" style="width:500px;"></center>
+
          <div id="sctarget1-1" class="myh2">Basic Part List</div>
−
              <center>Fig1. The mechanism of ELK16 system</center>
+
  
−
<div id="sctarget1-1" class="myh2">Basic Part List</div>
+
          <table width="100%">
 +
            <tr>
 +
              <th>Name</th>
 +
              <th>Type</th>
 +
              <th>Part Name</th>
 +
              <th width="500">Diagram</th>
 +
              <th>Designer</th>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="http://parts.igem.org/Part:BBa_K3815000">BBa_K3815000</a></td>
 +
              <td>Protein Coding</td>
 +
              <td>ELK16-PT Linker-Mxe GyrA intein</td>
 +
              <td>
 +
                <div class="figs">
 +
                  <img class="h70" src="https://static.igem.org/mediawiki/2021/c/cc/T--kyoto--plasmid_ELK16.png">
 +
                </div>
 +
              </td>
 +
              <td>Koudai Shiotsu</td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="http://parts.igem.org/Part:BBa_K3815001">BBa_K3815001</a></td>
 +
              <td>Protein Coding</td>
 +
              <td>ELK16-PT Linker-Mxe GyrA intein-CecropinA</td>
 +
              <td>
 +
                <div class="figs">
 +
                  <img class="h70" src="https://static.igem.org/mediawiki/2021/c/c0/T--kyoto--CecA-ELK16.png">
 +
                </div>
 +
              </td>
 +
              <td>Koudai Shiotsu</td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="http://parts.igem.org/Part:BBa_K3815002">BBa_K3815002</a></td>
 +
              <td>Protein Coding</td>
 +
              <td>ELK16-PT Linker-Mxe GyrA intein-Defensin1</td>
 +
              <td>
 +
                <div class="figs">
 +
                  <img class="h70" src="https://static.igem.org/mediawiki/2021/8/89/T--kyoto--Def1_ELK16.png">
 +
                </div>
 +
              </td>
 +
              <td>Koudai Shiotsu</td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="http://parts.igem.org/Part:BBa_K3815003">BBa_K3815003</a></td>
 +
              <td>Protein Coding</td>
 +
              <td>ELK16-PT Linker-Mxe GyrA intein-LL37</td>
 +
              <td>
 +
                <div class="figs">
 +
                  <img class="h70" src="https://static.igem.org/mediawiki/2021/e/e5/T--kyoto--LL37_ELK16.png">
 +
                </div>
 +
              </td>
 +
              <td>Koudai Shiotsu</td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="http://parts.igem.org/Part:BBa_K3815004">BBa_K3815004</a></td>
 +
              <td>Protein Coding</td>
 +
              <td>ELK16-PT Linker-Mxe GyrA intein-NOP1</td>
 +
              <td height="30">
 +
                <div class="figs">
 +
                  <img class="h70" src="https://static.igem.org/mediawiki/2021/3/3f/T--kyoto--NOP1_ELK16.png">
 +
                </div>
 +
              </td>
 +
              <td>Koudai Shiotsu</td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="http://parts.igem.org/Part:BBa_K3815005">BBa_K3815005</a></td>
 +
              <td>Protein Coding</td>
 +
              <td>ELK16-PT Linker-Mxe GyrA intein-NOP1v</td>
 +
              <td>
 +
                <div class="figs">
 +
                  <img class="h70" src="https://static.igem.org/mediawiki/2021/0/09/T--kyoto--NOP1v_ELK16.png">
 +
                </div>
 +
              </td>
 +
              <td>Koudai Shiotsu</td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="http://parts.igem.org/Part:BBa_K3815006">BBa_K3815006</a></td>
 +
              <td>Protein Coding</td>
 +
              <td>His tag-PT Linker-Mxe GyrA intein-CecropinA</td>
 +
              <td><img class="h70" src="https://static.igem.org/mediawiki/2021/a/a9/T--kyoto--CecA_His.png"></td>
 +
              <td>Hiroto Koga</td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="http://parts.igem.org/Part:BBa_K3815007">BBa_K3815007</a></td>
 +
              <td>Protein Coding</td>
 +
              <td>His tag-PT Linker-Mxe GyrA intein-Defensin1</td>
 +
              <td>
 +
                <div class="figs">
 +
                  <img class="h70" src="https://static.igem.org/mediawiki/2021/c/c0/T--kyoto--Def1_His.png">
 +
                </div>
 +
              </td>
 +
              <td>Hiroto Koga</td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="http://parts.igem.org/Part:BBa_K3815008">BBa_K3815008</a></td>
 +
              <td>Protein Coding</td>
 +
              <td>His tag-PT Linker-Mxe GyrA intein-LL37</td>
 +
              <td>
 +
                <div class="figs">
 +
                  <img class="h70" src="https://static.igem.org/mediawiki/2021/e/e1/T--kyoto--LL37_His.png">
 +
                </div>
 +
              </td>
 +
              <td>Hiroto Koga</td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="http://parts.igem.org/Part:BBa_K3815009">BBa_K3815009</a></td>
 +
              <td>Protein Coding</td>
 +
              <td>His tag-PT Linker-Mxe GyrA intein-NOP1</td>
 +
              <td>
 +
                <div class="figs">
 +
                  <img class="h70" src="https://static.igem.org/mediawiki/2021/5/5d/T--kyoto--NOP1_His.png">
 +
                </div>
 +
              </td>
 +
              <td>Hiroto Koga</td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="http://parts.igem.org/Part:BBa_K3815010">BBa_K3815010</a></td>
 +
              <td>Protein Coding</td>
 +
              <td>His tag-PT Linker-Mxe GyrA intein-NOP1v</td>
 +
              <td>
 +
                <div class="figs">
 +
                  <img class="h70" src="https://static.igem.org/mediawiki/2021/a/a6/T--kyoto--NOP1v_His.png">
 +
                </div>
 +
              </td>
 +
              <td>Hiroto Koga</td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="http://parts.igem.org/Part:BBa_K3815011">BBa_K3815011</a></td>
 +
              <td>RNA</td>
 +
              <td>V-ATPaseB </td>
 +
              <td>N/A</td>
 +
              <td>Yuto Ueda</td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="http://parts.igem.org/Part:BBa_K3815012">BBa_K3815012</a></td>
 +
              <td>RNA</td>
 +
              <td>TrxZ -3</td>
 +
              <td>N/A</td>
 +
              <td>Yuto Ueda</td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="http://parts.igem.org/Part:BBa_K3815013">BBa_K3815013</a></td>
 +
              <td>RNA</td>
 +
              <td>EPH1</td>
 +
              <td>N/A</td>
 +
              <td>Yuto Ueda</td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="http://parts.igem.org/Part:BBa_K3815032">BBa_K3815032</a></td>
 +
              <td>Protein Coding</td>
 +
              <td>B. stearothermophilus DNA polymerase</td>
 +
              <td>N/A</td>
 +
              <td>Paper</td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="http://parts.igem.org/Part:BBa_K3815033">BBa_K3815033</a></td>
 +
              <td>Protein Coding</td>
 +
              <td>HIV-1 reverse transcriptase p51</td>
 +
              <td>N/A</td>
 +
              <td>Paper</td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="http://parts.igem.org/Part:BBa_K3815034">BBa_K3815034</a></td>
 +
              <td>Protein Coding</td>
 +
              <td>HIV-1 reverse transcriptase p66</td>
 +
              <td>N/A</td>
 +
              <td>Paper</td>
 +
            </tr>
 +
          </table>
  
−
<table border="1" width="100%">
+
          <div id="sctarget2" class="myh1-green">Improvement of an Existing Part</div>
−
  <tr>
+
          <div id="sctarget2-x" class="myh2">Overview</div>
−
      <th>Name</th>
+
          <div class="myp">Each of the parts listed below are the improvement from <a
−
      <th>Type</th>
+
              href="http://parts.igem.org/Part:BBa_K1051206">BBa_K1051206</a>, the wildtype ssrA tag.
−
      <th>Part Name</th>
+
            Three C-terminal amino acids LAA in WT tag are replaced in these mutants, therefore
−
      <th width="500" >Diagram</th>
+
            resulting in a reduced protein degradation efficiency to various degrees. The collection of
−
      <th>Designer</th>
+
            these mutants consist of a large repertoire of protein degradation tags of various levels,
−
    </tr>
+
            which can be applied for fine-tuning of many kinds of synthetic systems that require a
−
    <tr>
+
            precise control of protein half-life (see ssrA results in <a
−
      <td><a href="http://parts.igem.org/Part:BBa_K3815000">BBa_K3815000</a></td>
+
              href="https://2021.igem.org/Team:Kyoto/Results">the Results page</a>).
−
      <td>Protein Coding</td>
+
          </div>
−
      <td>ELK16-PT Linker-Mxe Gyr intein</td>
+
−
      <td>
+
−
      <div class ="figs">
+
−
        <img class="h70" src="https://static.igem.org/mediawiki/2021/c/cc/T--kyoto--plasmid_ELK16.png">
+
−
      </div></td>
+
−
      <td>Koudai Shiotsu</td>
+
−
    </tr>
+
−
    <tr>
+
−
      <td><a href="http://parts.igem.org/Part:BBa_K3815001">BBa_K3815001</a></td>
+
−
      <td>Protein Coding</td>
+
−
      <td>ELK16-PT Linker-Mxe Gyr intein-CecropinA</td>
+
−
      <td>
+
−
      <div class ="figs">
+
−
        <img class="h70" src="https://static.igem.org/mediawiki/2021/c/c0/T--kyoto--CecA-ELK16.png">
+
−
      </div>
+
−
      </td>
+
−
      <td>Koudai Shiotsu</td>
+
−
    </tr>
+
−
  <tr>
+
−
      <td><a href="http://parts.igem.org/Part:BBa_K3815002">BBa_K3815002</a></td>
+
−
      <td>Protein Coding</td>
+
−
      <td>ELK16-PT Linker-Mxe Gyr intein-Defensin1</td>
+
−
      <td>
+
−
      <div class ="figs">
+
−
        <img class="h70" src="https://static.igem.org/mediawiki/2021/8/89/T--kyoto--Def1_ELK16.png">
+
−
      </div></td>
+
−
      <td>Koudai Shiotsu</td>
+
−
    </tr>
+
−
  <tr>
+
−
      <td><a href="http://parts.igem.org/Part:BBa_K3815003">BBa_K3815003</a></td>
+
−
      <td>Protein Coding</td>
+
−
      <td>ELK16-PT Linker-Mxe Gyr intein-LL37</td>
+
−
      <td>
+
−
      <div class ="figs">
+
−
        <img class="h70" src="https://static.igem.org/mediawiki/2021/e/e5/T--kyoto--LL37_ELK16.png">
+
−
      </div></td>
+
−
      <td>Koudai Shiotsu</td>
+
−
    </tr>
+
−
      <td><a href="http://parts.igem.org/Part:BBa_K3815004">BBa_K3815004</a></td>
+
−
      <td>Protein Coding</td>
+
−
      <td>ELK16-PT Linker-Mxe Gyr intein-NOP1</td>
+
−
      <td height="30">
+
−
      <div class ="figs">
+
−
        <img class="h70" src="https://static.igem.org/mediawiki/2021/3/3f/T--kyoto--NOP1_ELK16.png">
+
−
      </div></td>
+
−
      <td>Koudai Shiotsu</td>
+
−
    </tr>
+
−
    <tr>
+
−
      <td><a href="http://parts.igem.org/Part:BBa_K3815005">BBa_K3815005</a></td>
+
−
      <td>Protein Coding</td>
+
−
      <td>ELK16-PT Linker-Mxe Gyr intein-NOP1v</td>
+
−
      <td><div class ="figs">
+
−
        <img class="h70" src="https://static.igem.org/mediawiki/2021/0/09/T--kyoto--NOP1v_ELK16.png">
+
−
    </div></td>
+
−
      <td>Koudai Shiotsu</td>
+
−
  </tr>
+
−
  <tr>
+
−
      <td><a href="http://parts.igem.org/Part:BBa_K3815006">BBa_K3815006</a></td>
+
−
      <td>Protein Coding</td>
+
−
      <td>His tag-PT Linker-Mxe Gyr intein-CecropinA</td>
+
−
      <td><img class="h70" src="https://static.igem.org/mediawiki/2021/a/a9/T--kyoto--CecA_His.png">
+
−
    </div></td>
+
−
      <td>Hiroto Koga</td></tr>
+
−
  <tr>
+
−
      <td><a href="http://parts.igem.org/Part:BBa_K3815007">BBa_K3815007</a></td>
+
−
      <td>Protein Coding</td>
+
−
      <td>His tag-PT Linker-Mxe Gyr intein-Defensin1</td>
+
−
      <td><div class ="figs">
+
−
        <img class="h70" src="https://static.igem.org/mediawiki/2021/c/c0/T--kyoto--Def1_His.png">
+
−
    </div></td>
+
−
      <td>Hiroto Koga</td>
+
−
  </tr>
+
−
  <tr>
+
−
      <td><a href="http://parts.igem.org/Part:BBa_K3815008">BBa_K3815008</a></td>
+
−
      <td>Protein Coding</td>
+
−
      <td>His tag-PT Linker-Mxe Gyr intein-LL37</td>
+
−
      <td><div class ="figs">
+
−
        <img class="h70" src="https://static.igem.org/mediawiki/2021/e/e1/T--kyoto--LL37_His.png">
+
−
    </div></td>
+
−
      <td>Hiroto Koga</td>
+
−
  </tr>
+
−
  <tr>
+
−
      <td><a href="http://parts.igem.org/Part:BBa_K3815009">BBa_K3815009</a></td>
+
−
      <td>Protein Coding</td>
+
−
      <td>His tag-PT Linker-Mxe Gyr intein-NOP1</td>
+
−
      <td><div class ="figs">
+
−
        <img class="h70" src="https://static.igem.org/mediawiki/2021/5/5d/T--kyoto--NOP1_His.png">
+
−
    </div></td>
+
−
      <td>Hiroto Koga</td>
+
−
  </tr>
+
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815010">BBa_K3815010</a></td>
+
−
      <td>Protein Coding</td>
+
−
      <td>His tag-PT Linker-Mxe Gyr intein-NOP1v</td>
+
−
      <td><div class ="figs">
+
−
        <img class="h70" src="https://static.igem.org/mediawiki/2021/a/a6/T--kyoto--NOP1v_His.png">
+
−
    </div></td>
+
−
      <td>Hiroto Koga</td></tr>
+
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815011">BBa_K3815011</a></td>
+
−
      <td>RNA</td>
+
−
      <td>V-ATPaseB </td>
+
−
      <td></td>
+
−
      <td>Yuto Ueda</td></tr>
+
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815012">BBa_K3815012</a></td>
+
−
      <td>RNA</td>
+
−
      <td>TrxZ -3</td>
+
−
      <td></td>
+
−
      <td>Yuto Ueda</td></tr>
+
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815013">BBa_K3815013</a></td>
+
−
      <td>RNA</td>
+
−
      <td>EPH1</td>
+
−
      <td></td>
+
−
      <td>Yuto Ueda</td></tr>
+
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815014">BBa_K3815014</a></td>
+
−
      <td>Composite</td>
+
−
      <td>iRFP 670-superfolder GFP</td>
+
−
      <td></td>
+
−
      <td>Yuto Ueda</td></tr>
+
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815032">BBa_K3815032</a></td>
+
−
      <td>Protein Coding</td>
+
−
      <td>B. stearothermophilus DNA polymerase</td>
+
−
      <td></td>
+
−
      <td>Paper</td></tr>
+
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815033">BBa_K3815033</a></td>
+
−
      <td>Protein Coding</td>
+
−
      <td>HIV-1 reverse transcriptase p51</td>
+
−
      <td></td>
+
−
      <td>Paper</td></tr>
+
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815034">BBa_K3815034</a></td>
+
−
      <td>Protein Coding</td>
+
−
      <td>HIV-1 reverse transcriptase p66</td>
+
−
      <td></td>
+
−
      <td>Paper</td></tr>
+
−
  </table>
+
  
−
<div id="sctarget2" class="myh1-green">Improvement of an Existing Part</div>
+
          <div id="sctarget2-1" class="myh2">Improvement of an Existing Part List</div>
−
<div class="myp">Each of the parts listed below are the improvement from <a href="http://parts.igem.org/Part:BBa_K1051206">BBa_K1051206</a>, the wildtype ssrA tag. Three C-terminal amino acids LAA in WT tag are replaced in these mutants, therefore resulting in a reduced protein degradation efficiency to various degrees. The collection of these mutants consist of a large repertoire of protein degradation tags of various levels, which can be applied for fine-tuning of many kinds of synthetic systems that require a precise control of proteins (see ssrA results in <a class="wikinailink" href="https://2021.igem.org/Team:Kyoto/Results">the Results page</a>).
+
−
</div>
+
  
−
<div id="sctarget2-1" class="myh2">Improvement of an Existing Part List</div>
+
          <table width="100%">
−
 
+
            <tr>
−
<table border="1" width="100%">
+
              <th>Name</th>
−
  <tr>
+
              <th>Type</th>
−
      <th>Name</th>
+
              <th>Part Name</th>
−
      <th>Type</th>
+
              <th>Designer</th>
−
      <th>Part Name</th>
+
            </tr>
−
      <th>Designer</th>
+
            <tr>
−
    </tr>
+
              <td><a href="http://parts.igem.org/Part:BBa_K3815015">BBa_K3815015</a></td>
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815015">BBa_K3815015</a></td>
+
              <td>Tag</td>
−
      <td>Tag</td>
+
              <td>AANDENYALGA.mutant SsrA degradation tag</td>
−
      <td>AANDENYALGA.mutant SsrA degradation tag</td>
+
              <td>Alexander Liu</td>
−
      <td>Alexander Liu</td></tr>
+
            </tr>
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815016">BBa_K3815016</a></td>
+
            <tr>
−
      <td>Tag</td>
+
              <td><a href="http://parts.igem.org/Part:BBa_K3815016">BBa_K3815016</a></td>
−
      <td>AANDENYAKWA.mutant SsrA degradation tag</td>
+
              <td>Tag</td>
−
      <td>Alexander Liu</td></tr>
+
              <td>AANDENYAKWA.mutant SsrA degradation tag</td>
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815017">BBa_K3815017</a></td>
+
              <td>Alexander Liu</td>
−
      <td>Tag</td>
+
            </tr>
−
      <td>AANDENYALGALAK.mutant SsrA degradation tag</td>
+
            <tr>
−
      <td>Alexander Liu</td></tr>
+
              <td><a href="http://parts.igem.org/Part:BBa_K3815017">BBa_K3815017</a></td>
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815018">BBa_K3815018</a></td>
+
              <td>Tag</td>
−
      <td>Tag</td>
+
              <td>AANDENYALAK.mutant SsrA degradation tag</td>
−
      <td>AANDENYALGAHPK.mutant SsrA degradation tag</td>
+
              <td>Alexander Liu</td>
−
      <td>Alexander Liu</td></tr>
+
            </tr>
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815019">BBa_K3815019</a></td>
+
            <tr>
−
      <td>Tag</td>
+
              <td><a href="http://parts.igem.org/Part:BBa_K3815018">BBa_K3815018</a></td>
−
      <td>AANDENYALGALAL.mutant SsrA degradation tag</td>
+
              <td>Tag</td>
−
      <td>Alexander Liu</td></tr>
+
              <td>AANDENYAHPK.mutant SsrA degradation tag</td>
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815020">BBa_K3815020</a></td>
+
              <td>Alexander Liu</td>
−
      <td>Tag</td>
+
            </tr>
−
      <td>AANDENYALGANDK.mutant SsrA degradation tag</td>
+
            <tr>
−
      <td>Alexander Liu</td></tr>
+
              <td><a href="http://parts.igem.org/Part:BBa_K3815019">BBa_K3815019</a></td>
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815021">BBa_K3815021</a></td>
+
              <td>Tag</td>
−
      <td>Tag</td>
+
              <td>AANDENYALAL.mutant SsrA degradation tag</td>
−
      <td>AANDENYALGANEN.mutant SsrA degradation tag</td>
+
              <td>Alexander Liu</td>
−
      <td>Alexander Liu</td></tr>
+
            </tr>
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815022">BBa_K3815022</a></td>
+
            <tr>
−
      <td>Tag</td>
+
              <td><a href="http://parts.igem.org/Part:BBa_K3815020">BBa_K3815020</a></td>
−
      <td>AANDENYALGAYES.mutant SsrA degradation tag</td>
+
              <td>Tag</td>
−
      <td>Alexander Liu</td></tr>
+
              <td>AANDENYANDK.mutant SsrA degradation tag</td>
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815023">BBa_K3815023</a></td>
+
              <td>Alexander Liu</td>
−
      <td>Tag</td>
+
            </tr>
−
      <td>AANDENYALGAHAK.mutant SsrA degradation tag</td>
+
            <tr>
−
      <td>Alexander Liu</td></tr>
+
              <td><a href="http://parts.igem.org/Part:BBa_K3815021">BBa_K3815021</a></td>
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815024">BBa_K3815024</a></td>
+
              <td>Tag</td>
−
      <td>Tag</td>
+
              <td>AANDENYANEN.mutant SsrA degradation tag</td>
−
      <td>AANDENYALGALAS.mutant SsrA degradation tag</td>
+
              <td>Alexander Liu</td>
−
      <td>Alexander Liu</td></tr>
+
            </tr>
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815025">BBa_K3815025</a></td>
+
            <tr>
−
      <td>Tag</td>
+
              <td><a href="http://parts.igem.org/Part:BBa_K3815022">BBa_K3815022</a></td>
−
      <td>AANDENYALGATAP.mutant SsrA degradation tag</td>
+
              <td>Tag</td>
−
      <td>Alexander Liu</td></tr>
+
              <td>AANDENYAYES.mutant SsrA degradation tag</td>
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815026">BBa_K3815026</a></td>
+
              <td>Alexander Liu</td>
−
      <td>Tag</td>
+
            </tr>
−
      <td>AANDENYALGANAA.mutant SsrA degradation tag</td>
+
            <tr>
−
      <td>Alexander Liu</td></tr>
+
              <td><a href="http://parts.igem.org/Part:BBa_K3815023">BBa_K3815023</a></td>
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815027">BBa_K3815027</a></td>
+
              <td>Tag</td>
−
      <td>Tag</td>
+
              <td>AANDENYAHAK.mutant SsrA degradation tag</td>
−
      <td>AANDENYALGAKLA.mutant SsrA degradation tag</td>
+
              <td>Alexander Liu</td>
−
      <td>Alexander Liu</td></tr>  
+
            </tr>
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815028">BBa_K3815028</a></td>
+
            <tr>
−
      <td>Tag</td>
+
              <td><a href="http://parts.igem.org/Part:BBa_K3815024">BBa_K3815024</a></td>
−
      <td>AANDENYALGALPA.mutant SsrA degradation tag</td>
+
              <td>Tag</td>
−
      <td>Alexander Liu</td></tr>
+
              <td>AANDENYALAS.mutant SsrA degradation tag</td>
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815029">BBa_K3815029</a></td>
+
              <td>Alexander Liu</td>
−
      <td>Tag</td>
+
            </tr>
−
      <td>AANDENYALGALAM.mutant SsrA degradation tag</td>
+
            <tr>
−
      <td>Alexander Liu</td></tr>
+
              <td><a href="http://parts.igem.org/Part:BBa_K3815025">BBa_K3815025</a></td>
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815030">BBa_K3815030</a></td>
+
              <td>Tag</td>
−
      <td>Tag</td>
+
              <td>AANDENYATAP.mutant SsrA degradation tag</td>
−
      <td>AANDENYALGALAI.mutant SsrA degradation tag</td>
+
              <td>Alexander Liu</td>
−
      <td>Alexander Liu</td></tr>
+
            </tr>
−
  <tr><td><a href="http://parts.igem.org/Part:BBa_K3815031">BBa_K3815031</a></td>
+
            <tr>
−
      <td>Tag</td>
+
              <td><a href="http://parts.igem.org/Part:BBa_K3815026">BBa_K3815026</a></td>
−
      <td>AANDENYALGAPHA.mutant SsrA degradation tag</td>
+
              <td>Tag</td>
−
      <td>Alexander Liu</td></tr>
+
              <td>AANDENYANAA.mutant SsrA degradation tag</td>
−
</table>
+
              <td>Alexander Liu</td>
−
</div>
+
            </tr>
−
</div>
+
            <tr>
 +
              <td><a href="http://parts.igem.org/Part:BBa_K3815027">BBa_K3815027</a></td>
 +
              <td>Tag</td>
 +
              <td>AANDENYAKLA.mutant SsrA degradation tag</td>
 +
              <td>Alexander Liu</td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="http://parts.igem.org/Part:BBa_K3815028">BBa_K3815028</a></td>
 +
              <td>Tag</td>
 +
              <td>AANDENYALPA.mutant SsrA degradation tag</td>
 +
              <td>Alexander Liu</td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="http://parts.igem.org/Part:BBa_K3815029">BBa_K3815029</a></td>
 +
              <td>Tag</td>
 +
              <td>AANDENYALAM.mutant SsrA degradation tag</td>
 +
              <td>Alexander Liu</td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="http://parts.igem.org/Part:BBa_K3815030">BBa_K3815030</a></td>
 +
              <td>Tag</td>
 +
              <td>AANDENYALAI.mutant SsrA degradation tag</td>
 +
              <td>Alexander Liu</td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="http://parts.igem.org/Part:BBa_K3815031">BBa_K3815031</a></td>
 +
              <td>Tag</td>
 +
              <td>AANDENYAPHA.mutant SsrA degradation tag</td>
 +
              <td>Alexander Liu</td>
 +
            </tr>
 +
          </table>
 +
        </div>
 +
      </div>
 +
    </div>
  
−
</main>
+
  </main>
 
</html>
 
</html>
 
{{Kyoto/Footer}}
 
{{Kyoto/Footer}}

Latest revision as of 03:11, 12 December 2021

Parts
- Click to visit -
Basic Part
Overview
Our best basic part is BBa_K3815000. This is composed of ELK16, PT linker, Mxe GyrA intein. It can be expressed by attaching the sequence of the targeted protein to the N-terminus of Intein. This part is used for the purification method called ELK16system, which past iGEM teams had not been used. In this ELK16system, the ELK16 part is self-condensing, so the fusion protein can be extracted only by centrifugation, and the intein part is self-cleaved by DTT, so the target protein can be easily separated. The ELK16system will help improve the technology of recombinant protein purification, although it has some problems such as the presence of many other impurities in the protein recovery process.
Fig1. The mechanism of ELK16 system
Basic Part List
Name Type Part Name Diagram Designer
BBa_K3815000 Protein Coding ELK16-PT Linker-Mxe GyrA intein
Koudai Shiotsu
BBa_K3815001 Protein Coding ELK16-PT Linker-Mxe GyrA intein-CecropinA
Koudai Shiotsu
BBa_K3815002 Protein Coding ELK16-PT Linker-Mxe GyrA intein-Defensin1
Koudai Shiotsu
BBa_K3815003 Protein Coding ELK16-PT Linker-Mxe GyrA intein-LL37
Koudai Shiotsu
BBa_K3815004 Protein Coding ELK16-PT Linker-Mxe GyrA intein-NOP1
Koudai Shiotsu
BBa_K3815005 Protein Coding ELK16-PT Linker-Mxe GyrA intein-NOP1v
Koudai Shiotsu
BBa_K3815006 Protein Coding His tag-PT Linker-Mxe GyrA intein-CecropinA Hiroto Koga
BBa_K3815007 Protein Coding His tag-PT Linker-Mxe GyrA intein-Defensin1
Hiroto Koga
BBa_K3815008 Protein Coding His tag-PT Linker-Mxe GyrA intein-LL37
Hiroto Koga
BBa_K3815009 Protein Coding His tag-PT Linker-Mxe GyrA intein-NOP1
Hiroto Koga
BBa_K3815010 Protein Coding His tag-PT Linker-Mxe GyrA intein-NOP1v
Hiroto Koga
BBa_K3815011 RNA V-ATPaseB N/A Yuto Ueda
BBa_K3815012 RNA TrxZ -3 N/A Yuto Ueda
BBa_K3815013 RNA EPH1 N/A Yuto Ueda
BBa_K3815032 Protein Coding B. stearothermophilus DNA polymerase N/A Paper
BBa_K3815033 Protein Coding HIV-1 reverse transcriptase p51 N/A Paper
BBa_K3815034 Protein Coding HIV-1 reverse transcriptase p66 N/A Paper
Improvement of an Existing Part
Overview
Each of the parts listed below are the improvement from BBa_K1051206, the wildtype ssrA tag. Three C-terminal amino acids LAA in WT tag are replaced in these mutants, therefore resulting in a reduced protein degradation efficiency to various degrees. The collection of these mutants consist of a large repertoire of protein degradation tags of various levels, which can be applied for fine-tuning of many kinds of synthetic systems that require a precise control of protein half-life (see ssrA results in the Results page).
Improvement of an Existing Part List
Name Type Part Name Designer
BBa_K3815015 Tag AANDENYALGA.mutant SsrA degradation tag Alexander Liu
BBa_K3815016 Tag AANDENYAKWA.mutant SsrA degradation tag Alexander Liu
BBa_K3815017 Tag AANDENYALAK.mutant SsrA degradation tag Alexander Liu
BBa_K3815018 Tag AANDENYAHPK.mutant SsrA degradation tag Alexander Liu
BBa_K3815019 Tag AANDENYALAL.mutant SsrA degradation tag Alexander Liu
BBa_K3815020 Tag AANDENYANDK.mutant SsrA degradation tag Alexander Liu
BBa_K3815021 Tag AANDENYANEN.mutant SsrA degradation tag Alexander Liu
BBa_K3815022 Tag AANDENYAYES.mutant SsrA degradation tag Alexander Liu
BBa_K3815023 Tag AANDENYAHAK.mutant SsrA degradation tag Alexander Liu
BBa_K3815024 Tag AANDENYALAS.mutant SsrA degradation tag Alexander Liu
BBa_K3815025 Tag AANDENYATAP.mutant SsrA degradation tag Alexander Liu
BBa_K3815026 Tag AANDENYANAA.mutant SsrA degradation tag Alexander Liu
BBa_K3815027 Tag AANDENYAKLA.mutant SsrA degradation tag Alexander Liu
BBa_K3815028 Tag AANDENYALPA.mutant SsrA degradation tag Alexander Liu
BBa_K3815029 Tag AANDENYALAM.mutant SsrA degradation tag Alexander Liu
BBa_K3815030 Tag AANDENYALAI.mutant SsrA degradation tag Alexander Liu
BBa_K3815031 Tag AANDENYAPHA.mutant SsrA degradation tag Alexander Liu
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