Difference between revisions of "Team:GreatBay SCIE/Notebook"

 
(36 intermediate revisions by 4 users not shown)
Line 1: Line 1:
 +
{{GreatBay_SCIE/HTML/loader}}
 
<html>
 
<html>
 
<head>
 
<head>
Line 16: Line 17:
 
<body style="overflow-x:hidden;">
 
<body style="overflow-x:hidden;">
  
−
<div class="navbarsticker" id="nb" style="margin-top:10px;"></div>
+
<!--<div class="navbarsticker" id="nb" style="margin-top:10px;"></div>-->
−
<img src="https://static.igem.org/mediawiki/2021/b/ba/T--GreatBay_SCIE--Description_Header.png" class="header" style="margin-top:-8px">
+
<!--<img src="https://static.igem.org/mediawiki/2021/b/ba/T--GreatBay_SCIE--Description_Header.png" class="header" style="margin-top:-8px">-->
 
<div class="navbarsticker" id="navbarsticker" style="margin-top:10px;"></div>
 
<div class="navbarsticker" id="navbarsticker" style="margin-top:10px;"></div>
 
<div class="navbar" id="navbar">
 
<div class="navbar" id="navbar">
  
−
<a href="https://2021.igem.org/Team:GreatBay_SCIE">
+
<a href="https://2021.igem.org/Team:GreatBay_SCIE" style="padding-right:0px">
−
<img class="plogo" id="project_logo" src="https://static.igem.org/mediawiki/2021/6/6a/T--GreatBay_SCIE--Project_Logo.png" alt="logo" height="46"
+
<img class="plogo" id="project_logo" src="https://static.igem.org/mediawiki/2021/6/6a/T--GreatBay_SCIE--Project_Logo.png" alt="logo" height="46" style="position:absolute; margin-left:50px; margin-top:10px; left:0px;">
−
height:"46"; style="position:absolute; margin-left:30px; margin-top:10px;">
+
<img class="tlogo" id="team_logo" src="https://static.igem.org/mediawiki/2021/0/06/T--GreatBay_SCIE--Team_Logo.png" alt="logo" height="46" style="position:absolute; left:0; margin-top:13px!important; left:0px;">
−
<img class="tlogo" id="team_logo" src="https://static.igem.org/mediawiki/2021/0/06/T--GreatBay_SCIE--Team_Logo.png" alt="logo" height="46" style="position:absolute; left:0; margin-top:13px!important;">
+
 
</a>
 
</a>
−
<ul style="margin:0;">   
+
<ul style="margin-left: -11px">   
 
         <li>
 
         <li>
 
             <a href="https://2021.igem.org/Team:GreatBay_SCIE">Home</a>
 
             <a href="https://2021.igem.org/Team:GreatBay_SCIE">Home</a>
Line 40: Line 40:
 
             <a href="https://2021.igem.org/Team:GreatBay_SCIE/Description">Project</a>
 
             <a href="https://2021.igem.org/Team:GreatBay_SCIE/Description">Project</a>
 
             <ul>
 
             <ul>
−
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Description">Description</a></li>                 
+
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Description">Description</a></li>                
−
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Proof_Of_Concept">Proposed Implementation</a></li>   
+
               
−
                <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Parts">Parts</a></li>
+
                
−
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Engineering">Engineering</a></li>
+
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Design">Design</a></li>
−
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Design">Design</a></li>      
+
        <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Proof_Of_Concept">Proof of Concept</a></li>   
 +
        <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Results">Results</a></li>   
 +
        <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Engineering">Engineering</a></li>
 +
        <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Parts">Parts</a></li>
 +
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Implementation">Implementation</a></li>  
 +
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Contribution">Contribution</a></li>  
 
             </ul>
 
             </ul>
 
         </li>
 
         </li>
Line 50: Line 55:
 
             <a href="https://2021.igem.org/Team:GreatBay_SCIE/Experiments">Lab</a>
 
             <a href="https://2021.igem.org/Team:GreatBay_SCIE/Experiments">Lab</a>
 
             <ul>
 
             <ul>
 +
                <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Experiments">Experimental Design</a></li>
 +
                <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Results">Results</a></li> 
 
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Safety">Safety</a></li>
 
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Safety">Safety</a></li>
−
                <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Results">Results</a></li>
 
−
                <!--<li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Measurement">Measurement</a></li>-->
 
 
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Notebook">Lab Notes</a></li>
 
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Notebook">Lab Notes</a></li>
 +
                <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Protocol">Protocol</a></li>
 +
                <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Measurement">Measurement</a></li>
  
 
             </ul>
 
             </ul>
Line 61: Line 68:
 
             <ul>
 
             <ul>
 
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Model">Overview</a></li>
 
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Model">Overview</a></li>
−
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Optimum_Aptamer_Concentration">Optimum Aptamer Concentration</a></li>
+
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Optimum_Aptamer_Concentration" >Optimum Liposome-Aptamer Ratio</a></li>
 
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Drug_Loading">Drug Loading</a></li>
 
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Drug_Loading">Drug Loading</a></li>
 +
                <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Dissociation_constant">Dissociation Constant</a></li>
 +
<li><a href="https://2021.igem.org/Team:GreatBay_SCIE/3D_Model">3D Models</a></li>
 +
 
             </ul>
 
             </ul>
 
         </li>
 
         </li>
Line 68: Line 78:
 
             <a href="https://2021.igem.org/Team:GreatBay_SCIE/Human_Practices">Human Practices</a>
 
             <a href="https://2021.igem.org/Team:GreatBay_SCIE/Human_Practices">Human Practices</a>
 
             <ul>
 
             <ul>
−
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Education">Public Engagement & Education</a></li>
+
<li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Human_Practices">Integrated Human Practices</a></li>
−
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/IHP">Integrated Human Practices</a></li>
+
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Communication">Public Engagement & Education</a></li>
 +
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/BreastCancerInChina">GreatBay_SCIE X Pink Ribbon</a></li>
 
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Collaborations">Collaborations</a></li>
 
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Collaborations">Collaborations</a></li>
−
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/SMS">Partnership with Shenzhen_SMS</a></li>
+
                 <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Partnership">Partnership with SMS_Shenzhen</a></li>
 
             </ul>  
 
             </ul>  
 
         </li>
 
         </li>
−
        <!--<li>
 
−
            <a href="https://2021.igem.org/Team:GreatBay_SCIE/Notebook">Notebook</a>
 
−
            <ul>
 
−
                <li><a href="https://2021.igem.org/Team:GreatBay_SCIE/Methods">Methods</a></li>
 
  
−
        </li>-->
 
 
     </ul>
 
     </ul>
 
</div>
 
</div>
Line 85: Line 91:
  
  
−
<div class="sidebarsticker" id="sidebarsticker"></div>
+
<!--<div class="sidebarsticker" id="sidebarsticker"></div>-->
−
<div class="sidebar_outer" id="sidebar_inner">
+
<div class="sidebar_outer" id="sidebar_inner"style="width: 20%;height: auto!important;margin-left: 3%;border-radius:10px; position:fixed; margin-top: 150px;">
 +
<img src="https://static.igem.org/mediawiki/2021/9/9c/T--GreatBay_SCIE--Cat.gif" id="cat">
 
   <ul>
 
   <ul>
 
         <li class="top1">  
 
         <li class="top1">  
 
             <a href="#Aptamer" class="lateral inner-link";>Aptamer Lab Notebook</a>
 
             <a href="#Aptamer" class="lateral inner-link";>Aptamer Lab Notebook</a>
−
        </li>    
+
<ul>
−
        <li>  
+
<a href="#ELONA" class="lateral inner-link";>ELONA</a>
−
            <a href="#Questionnaire" class="lateral inner-link";>Questionnaire</a>
+
</ul>
−
        </li>  
+
<ul>
 +
<a href="#Asymmetric PCR" class="lateral inner-link";>Asymmetric PCR</a>
 +
</ul>
 
         <li>  
 
         <li>  
−
             <a href="#Self Education" class="lateral inner-link";>Self Education</a>
+
             <a href="#PLGA" class="lateral inner-link";>PLGA Lab Notebook</a>
 
         </li>  
 
         </li>  
 
         <li>  
 
         <li>  
−
             <a href="#Synthetic Biology Card Game" class="lateral inner-link";>Synthetic Biology Card Game</a>
+
             <a href="#Liposome" class="lateral inner-link";>Liposome Lab Notebook</a>
 
         </li>  
 
         </li>  
−
         <li>
+
         <li class="btm1">  
−
            <a href="#Education" class="lateral inner-link";>Synthetic Biology Education</a>
+
             <a href="#Cell" class="lateral inner-link";>Cell Room Notebook</a>
−
        </li>
+
−
        <li>  
+
−
             <a href="#TED Talk" class="lateral inner-link";>TED Talk</a>
+
 
         </li>  
 
         </li>  
 
     </ul>
 
     </ul>
 +
<div class="top_bt">
 +
<img  onclick="backtotop()" src="https://static.igem.org/mediawiki/2021/9/94/T--GreatBay_SCIE--Button.png"></img>
 +
</div>
 +
 
</div>
 
</div>
 
</div>
 
</div>
Line 112: Line 122:
  
 
<div class="content" style="font-family:"Century Gothic";">
 
<div class="content" style="font-family:"Century Gothic";">
−
<div class="title"
+
<div class="title"style="font-size:500% !important; text-align:center;">Lab Notebook</div><br>
−
<p>Lab Notebook</p>
+
 
−
</div>
+
−
<a class="anchorOffset" id="Aptamer"></a>
+
 
<h1 id='Aptamer'>Aptamer Lab Note</h1>
 
<h1 id='Aptamer'>Aptamer Lab Note</h1>
  
−
<h4>(Scroll down to see more in the pdf below)</h4>
+
<h2 id="ELONA">ELONA</h2>
 +
<p>(Scroll down to see more in the pdf below)
 +
<br>
 +
All raw data for aptamer lab are compiled <a href="https://2021.igem.org/File:T--GreatBay_SCIE--Aptamer_ELONA_Lab.zip" target = "_blank">here</a><br>
  
−
<iframe src="https://static.igem.org/mediawiki/2021/2/2b/T--GreatBay_SCIE--AptamerLabNote.pdf" width="90%" height="600px">
+
<iframe src="https://static.igem.org/mediawiki/2021/2/2b/T--GreatBay_SCIE--AptamerLabNote.pdf" width="90%" height="600px"></iframe>
−
</div>
+
</p>
−
<p>&nbsp;</p>
+
 
−
<ul>
+
<h2 id="Asymmetric PCR">Asymmetric PCR</h2>
−
<a class="anchorOffset" id="Questionnaire"></a>
+
 
−
<h1 id="Questionnaire">Questionnaire </h1>
+
<iframe src="https://static.igem.org/mediawiki/2021/c/cc/T--GreatBay_SCIE--AS_PCR_Lab_Notes.pdf" width="90%" height="600px"></iframe>
−
<p>After our interests of breast cancer was intrigued, we started different types of investigation. First, we had obtained online data regarding the general influence and severity of the disease. Realizing that this disease is on the top of the list among all illnesses exposed to women occupying 24.2% of incidence, we were worried about the possible lack of awareness to prevent and to deal with cancer in an appropriate way, especially when it comes to breast cancer. Then, we designed a survey and distributed it to 607 people. In fact, we have expected similar result that tends to reflect the lack of knowledge about breast cancer; however, the extent shocked us. Over half of the people being investigated acknowledged that they haven’t learned anything in breast cancer yet, and a lot of them are unfamiliar with commonsense such as what might lead to the danger of breast cancer, how is breast cancer developed, will it be spread infectiously among individuals. Apart from that, the cancer treatment is also the area of unknown for a lot of survey fillers. Based on the results, we decided to plan a series of activities to learn more details about breast cancer ourselves and then transfer the knowledge to others. </p>
+
<h1 id='PLGA'>PLGA Lab Notebook</h1>
−
<p>&nbsp;</p>
+
<iframe src="https://static.igem.org/mediawiki/2021/6/6a/T--GreatBay_SCIE--PLGA_Labnotes.pdf" width="90%" height="600px"></iframe>
−
<a class="anchorOffset" id="Synthetic Biology Card Game"></a>
+
 
−
<h1 id='Synthetic Biology Card Game'>Synthetic Biology Card Game</h1>
+
<h1 id='Liposome'>Liposome Lab Notebook</h1>
−
<p>To promote breast cancer, our team designed our own card game. Each card has different information ranging from the causes of breast cancer to obstacles patients might encounter along with the treatment processes. At first, we played this card game with students in the IGEM club. Some students thought this card game was a good way for people to learn more about breast cancer. For instance, initially many students were not aware that endocrine dyscrasia and persistent emotional fluctuations are associated with high risks of breast cancer,but after this card game, they gained a new understanding of potential factors contributing to breast cancers. The card game also introduced students to possible preventions of breast cancers like regular self-checking and early treatments. After this card game, our team also reinforced the importance of self-checking and regular checking in preventing breasts cancers. This card game received positive feedback from students and raised people&#39;s awareness about breast cancer intriguingly. Hoping to engage more people, our team decided to refine the card games and give more people chances to get involved in breast cancer. </p>
+
 
−
<img src="https://static.igem.org/mediawiki/2021/4/41/T--GreatBay_SCIE--CardGame.jpg"/ width="100%">
+
<iframe src="https://static.igem.org/mediawiki/2021/a/a0/T--GreatBay_SCIE--Liposome_Notebook.pdf" width="90%" height="600px"></iframe>
−
<h6>Fig.1: From design to product</h6>
+
 
−
<img src="https://static.igem.org/mediawiki/2021/a/a6/T--GreatBay_SCIE--CardGame2.jpg"/ width="100%">
+
<h1 id='Cell'>Cell Room Notebook</h1>
−
<h6>Fig.2: Playing the card game during the lab tour</h6>
+
<iframe src="https://static.igem.org/mediawiki/2021/d/d2/T--GreatBay_SCIE--Cell_Room_Lab_Notes.pdf" width="90%" height="600px"></iframe>
−
<p>&nbsp;</p>
+
 
−
<a class="anchorOffset" id="Education"></a>
+
<br>
−
<h1 id="Education">Synthetic biology education in SCIE</h1>
+
<br>
−
<p>After the beginning of the school, we restarted the IGEM club that opened last year in SCIE so as to circulate the knowledge of Synthetic Biology with our peers. Therefore, we conducted a series of lectures about Synthetic Biology, Bio-engineering, and scientific research skills to teach our younger peers in SCIE. </p>
+
−
<p>Our members has prepared a lot for these lectures. We have covered many things, including basic knowledge of DNA structure and central dogma of DNA replication, transcription and translation. We also introduced them about practical part of synthetic biology by playing the video which manipulates the real PCR and Agarose Gel Electropherosis experiment. What’s more, the synthetic biology lab belonging to SCIE was still constructing. We promised our members that after the completion of the construction, we would show them around the lab and teach them how to do those experiments in the real life under the supervision of school tutor if we got the chance. </p>
+
−
<p>Beside the knowledge, we also provided full-scaled information about IGEM and shared our precious experiences as well as heartfelt feeling of participating IGEM. We hoped that the spark of love for synthetic biology and the spirit of curiosity and exploration could be inherited by juniors. </p>
+
−
<img src="https://static.igem.org/mediawiki/2021/6/61/T--GreatBay_SCIE--education.jpg"/ width="100%">
+
−
<p>&nbsp;</p>
+
−
<a class="anchorOffset" id="TED Talk"></a>
+
−
<h1 id="TED Talk">Lecture within iGEM Club</h1>
+
−
<p>To further raise people’s awareness about breast cancer, we held lectures at our school. Our lectures introduced students to potential causes of breast cancer and preventions. In the hope of engaging more students, we tailored our lectures to what they are interested in such as different treatments for breast cancers. We also shared real stories about breast cancer patients with students to reinforce the importance of paying attention to breast health from a young age. </p>
+
−
<p>&nbsp;</p>
+
−
<a class="anchorOffset" id="Lab tour"></a>
+
−
<h1 id="Lab tour">Lab tour</h1>
+
−
<p>We would like to offer an opportunity for our IGEM club members to visit to the laboratory in the real life. However, the construction of our own school lab was predicted to be completed in the next year.  Thus, we prepared a lab tour for our IGEM club members to visit to the Bluepha lab, which was also the place we do experiments of our IGEM project. We hoped this special lab tour experience could help our members gain a deeper insights in the actual synthetic biology experiement. What&#39;s more,  being familiar with experimental operations is a very basic point for learning synthetic biology and participating in igem competitions. If they want to participate in the IGEM next year, this experience will help them build a strong fundation.</p>
+
−
<img src="https://static.igem.org/mediawiki/2021/8/8f/T--GreatBay_SCIE--labtour.jpg"/ width="100%">
+
−
<p>&nbsp;</p>
+
−
<h1>Project introduction via Wechat Official Account</h1>
+
−
<p>给Alice写</p>
+
−
<h1>Presentation in Bluepha lab summer camp</h1>
+
−
<p>Jerry and Albert </p>
+
 
<script type="text/javascript" src="https://2021.igem.org/wiki/index.php?title=Template:GreatBay_SCIE/JS/sidebar&action=raw&ctype=text/javascript"></script>
 
<script type="text/javascript" src="https://2021.igem.org/wiki/index.php?title=Template:GreatBay_SCIE/JS/sidebar&action=raw&ctype=text/javascript"></script>
 
</body>
 
</body>
 
</html>
 
</html>

Latest revision as of 02:05, 22 October 2021

Lab Notebook

Aptamer Lab Note

ELONA

(Scroll down to see more in the pdf below)
All raw data for aptamer lab are compiled here

Asymmetric PCR

PLGA Lab Notebook

Liposome Lab Notebook

Cell Room Notebook