Difference between revisions of "Team:Shanghai Metro HS/Results"

(Prototype team page)
 
 
(3 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
{{IGEM_TopBar}}
+
<html lang="en">
−
{{Shanghai_Metro_HS}}
+
−
<html>
+
−
 
+
−
<div class="column full_size">
+
−
<h1>Results</h1>
+
−
<p>You can describe the results of your project and your future plans here. </p>
+
−
</div>
+
−
 
+
−
 
+
−
<div class="column third_size" >
+
−
 
+
−
<h3>What should this page contain?</h3>
+
−
<ul>
+
−
<li> Clearly and objectively describe the results of your work.</li>
+
−
<li> Future plans for the project. </li>
+
−
<li> Considerations for replicating the experiments. </li>
+
−
</ul>
+
−
</div>
+
−
 
+
−
 
+
−
 
+
−
 
+
−
<div class="column two_thirds_size" >
+
−
<h3>Describe what your results mean </h3>
+
−
<ul>
+
−
<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
+
−
<li> Show data, but remember <b>all measurement and characterization data must also be on the Part's Main Page on the <a href="http://parts.igem.org/Main_Page">Registry</a>.</b> Otherwise these data will not be in consideration for any medals or part awards! </li>
+
−
<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
+
−
</ul>
+
−
</div>
+
−
 
+
−
 
+
−
<div class="clear extra_space"></div>
+
−
 
+
−
 
+
−
 
+
−
<div class="column two_thirds_size" >
+
−
<h3> Project Achievements </h3>
+
−
 
+
−
<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
+
−
 
+
−
<ul>
+
−
<li>A list of linked bullet points of the successful results during your project</li>
+
−
<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
+
−
</ul>
+
−
 
+
−
</div>
+
−
 
+
−
 
+
−
 
+
−
<div class="column third_size" >
+
−
<div class="highlight decoration_A_full">
+
−
<h3>Inspiration</h3>
+
−
<p>See how other teams presented their results.</p>
+
−
<ul>
+
−
<li><a href="https://2019.igem.org/Team:Newcastle/Results">2019 Newcastle</a></li>
+
−
<li><a href="https://2019.igem.org/Team:Munich/Results">2019 Munich </a></li>
+
−
<li><a href="https://2019.igem.org/Team:Tec-Chihuahua/Results">2019 Tec Chihuahua</a></li>
+
−
<li><a href="https://2020.igem.org/Team:Aalto-Helsinki/Results">2020 Aalto Helsinki</a></li>
+
−
<li><a href="https://2020.igem.org/Team:GreatBay_SCIE/Results">2020 GreatBay SCIE</a></li>
+
−
<li><a href="https://2020.igem.org/Team:Queens_Canada/Results">2020 Queens Canada</a></li>
+
−
 
+
−
</ul>
+
−
</div>
+
−
</div>
+
−
 
+
  
 +
<head>
 +
    <meta charset="UTF-8">
 +
    <meta http-equiv="X-UA-Compatible" content="IE=edge">
 +
    <meta name="viewport" content="width=device-width, initial-scale=1.0">
 +
    <title>Shanghai_Metro_HS</title>
 +
    <link rel="stylesheet"
 +
        href="https://2021.igem.org/wiki/index.php?title=Template:Shanghai_Metro_HS/Main_CSS&action=raw&ctype=text/css" />
  
 +
</head>
  
 +
<body>
 +
    <nav class="head-nav clearfix">
 +
        <div class="top-block"></div>
 +
        <div class="top-nav-bar">
 +
            <ul class="clearfix">
 +
                <span class="small-logo"></span>
 +
                <li>
 +
                    <a href="https://2021.igem.org/Team:Shanghai_Metro_HS">Home</a>
 +
                </li>
 +
                <li class="active">
 +
                    <a href="">Project</a>
 +
                    <div class="sub-nav">
 +
                        <ul>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Description"
 +
                                    class="sub-nav-74">Description</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Experiments"
 +
                                    class="sub-nav-74">Experiments</a></li>
 +
                            <li class="current-sub-nav"><a href="#" class="sub-nav-74">Results</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Proof_Of_Concept"
 +
                                    class="sub-nav-52">Proof Of Concept</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Notebook"
 +
                                    class="sub-nav-52">Notebook</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Safety">Safety</a></li>
 +
                        </ul>
 +
                    </div>
 +
                </li>
 +
                <li>
 +
                    <a href="">Parts</a>
 +
                    <div class="sub-nav">
 +
                        <ul>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Parts" class="sub-nav-74">Parts
 +
                                    Collection</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Engineering"
 +
                                    class="sub-nav-74">Engineering</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Contribution"
 +
                                    class="sub-nav-74">Contribution</a></li>
 +
                        </ul>
 +
                    </div>
 +
                </li>
 +
                <li>
 +
                    <a href="">Human Practices</a>
 +
                    <div class="sub-nav">
 +
                        <ul>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Human_Practices"
 +
                                    class="sub-nav-74">Integrated Human Practice</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Communication"
 +
                                    class="sub-nav-74">Communication</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Fundraising"
 +
                                    class="sub-nav-74">Fundraising</a></li>
 +
                        </ul>
 +
                    </div>
 +
                </li>
 +
                <li>
 +
                    <a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Implementation">Implementation</a>
 +
                </li>
 +
                <li>
 +
                    <a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Entrepreneurship">Entrepreneurship</a>
 +
                </li>
 +
                <li>
 +
                    <a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Model">Model</a>
 +
                </li>
 +
                <li>
 +
                    <a href="">Team</a>
 +
                    <div class="sub-nav">
 +
                        <ul>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Members" class="sub-nav-74">Team
 +
                                    Members</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Attributions"
 +
                                    class="sub-nav-74">Attributions</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Collaborations"
 +
                                    class="sub-nav-74">Collaborations</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Partnership"
 +
                                    class="sub-nav-74">Partnership</a></li>
 +
                        </ul>
 +
                    </div>
 +
                </li>
 +
            </ul>
 +
        </div>
 +
    </nav>
 +
    <div class="sub-banner">
 +
        <img src="https://static.igem.org/mediawiki/2021/a/a4/T--Shanghai_Metro_HS--Team_Members01.png" alt="" />
 +
        <span>Results</span>
 +
    </div>
 +
    <div class="sub-content">
 +
        <div class="img-wrap no-margin">
 +
            <img tyle="width: 816px;" src="https://static.igem.org/mediawiki/2021/d/d6/T--Shanghai_Metro_HS--results01.jpg"
 +
                alt="" />
 +
            <span>Figure 1. The result of enzyme cutting and PCR</span>
 +
        </div>
 +
        <div class="article-content" style="font-size: 18px;">This picture is the nucleic acid electrophoresis result of
 +
            enzyme cutting and
 +
            PCR(By performing PCR, we can obtain the desirable PKC-OP’s gene segment.). Column “marker” is a column that
 +
            is used to show the position of different lengths of genes. In this step, our aim is to verify whether these
 +
            results are the desired ones.
 +
            In figure 1, the number labeled above figure 1 represents different results. To begin with, number 1 and 2
 +
            is the result for pET-25b after enzyme cutting. Additionally, number 3 is the pET-25b without enzyme
 +
            cutting. At last, number 4 and 5 is the PKC-OP after PCR. As our experiment moves on, the data of our PKC-OP
 +
            and pET-25b are 233.2ng/μL and 17.8ng/μL. Lastly, we use the homologous combination to combine them.
 +
        </div>
 +
        <div class="img-wrap no-margin">
 +
            <img tyle="width: 816px;" src="https://static.igem.org/mediawiki/2021/6/61/T--Shanghai_Metro_HS--results02.jpg"
 +
                alt="" />
 +
            <span>Figure 2 E. coil having the desired pET-25b and PKC-OP </span>
 +
        </div>
 +
        <div class="article-content">The pET25b-PKC-OP was constructed.</div>
 +
        <div class="article-content" style="font-size: 18px;">The plate shows monoclonals of pET25b-PKC-OP constructs
 +
        </div>
 +
        <div class="img-wrap no-margin">
 +
            <img tyle="width: 816px;" src="https://static.igem.org/mediawiki/2021/b/b3/T--Shanghai_Metro_HS--results03.jpg"
 +
                alt="" />
 +
            <span>Figure 3. The result of Apa1 enzyme digestion validation</span>
 +
        </div>
 +
        <div class="article-content" style="font-size: 18px;">
 +
            Column “marker” is a column that is used to show the position of different lengths of genes. Number 1 to 10
 +
            is the result for recombinant plasmid pET25b-PKC-OP after Apa1 enzyme digestion. We get two bands of 4712bp
 +
            and 1894bp. It further indicates that the obtained monoclonals were positive monoclonals containing the
 +
            recombinant plasmid.</div>
 +
        <div class="article-content" style="font-size: 18px;">Future plan: Testing the effectiveness of producing
 +
            cellulase of positive
 +
            recombinant bacteria. This is important because we need to know how much bacteria is needed to be used to
 +
            effectively help animals digest fodder. In this experiment, we can control the time that positive
 +
            recombinant bacteria grow and use the OD test to set the numbers of bacteria. Then put different numbers of
 +
            bacteria and cellulose filter paper together. OD test can be used again to see the glucose concentration.
 +
        </div>
 +
        <div class="img-wrap no-margin">
 +
            <img style="width: 816px;" src="https://static.igem.org/mediawiki/2021/5/55/T--Shanghai_Metro_HS--chgt3.jpg"
 +
                alt="" />
 +
        </div>
 +
        <div class="img-wrap no-margin">
 +
            <img style="width: 816px;" src="https://static.igem.org/mediawiki/2021/7/78/T--Shanghai_Metro_HS--chgt4.jpg"
 +
                alt="" />
 +
        </div>
 +
        <div class="article-content" style="font-size: 18px;">
 +
            We designed several different induction conditions for protein expression. As the vector we chose to
 +
            construct the plasmid contains a pelB signal peptide, the engineered strain would secret the protein into
 +
            the medium. Therefore, we collected the culture supernatant after induction and ran SDS-PAGE for
 +
            verification.
 +
            According to the calculation by Snapgene, the expected protein molecular shall weigh 45.6 KDa. As seen from
 +
            the SDS-PAGE map (Fig. 4), there is a wide band just above 40 KDa and it indicates that we have obtained the
 +
            PKC-OP protein.
 +
        </div>
 +
        <div class="article-title">Future Plan </div>
 +
        <div class="article-content" style="font-size: 18px;">Testing the effectiveness of producing cellulase of
 +
            positive recombinant bacteria.
 +
            This is important because we need to know how much bacteria is needed to be used to effectively help animals
 +
            digest fodder. In this experiment, we can control the time that positive recombinant bacteria grow and use
 +
            the OD test to set the numbers of bacteria. Then put different numbers of bacteria and cellulose filter
 +
            paper together. OD test can be used again to see the glucose concentration. </div>
 +
    </div>
 +
    <footer class="footer">
 +
        <section class="footer-wrap">
 +
            <div class="footer-contact">CONTACT INFO</div>
 +
            <p class="margin-bottom-20">Email: igemteamceres@gmail.com</p>
 +
            <p><i style="color: #2d2d2d;">WeChat Official Account: iGEM Ceres</i></p>
 +
        </section>
 +
    </footer>
 +
</body>
 +
<script>
 +
    let liTags = document.querySelectorAll(".top-nav-bar > ul > li");
 +
    let len = liTags.length;
 +
    for (let i = 0; i < len; i++) {
 +
        liTags[i].onclick = function (e) {
 +
            //先移除所有的点击样式
 +
            for (let j = 0; j < len; j++) {
 +
                liTags[j].classList.remove("active");
 +
            }
 +
            //再添加点击样式
 +
            let li = e.currentTarget;
 +
            li.classList.add("active");
 +
        }
 +
    }
 +
</script>
  
 
</html>
 
</html>

Latest revision as of 13:02, 20 October 2021

Shanghai_Metro_HS

Results
Figure 1. The result of enzyme cutting and PCR
This picture is the nucleic acid electrophoresis result of enzyme cutting and PCR(By performing PCR, we can obtain the desirable PKC-OP’s gene segment.). Column “marker” is a column that is used to show the position of different lengths of genes. In this step, our aim is to verify whether these results are the desired ones. In figure 1, the number labeled above figure 1 represents different results. To begin with, number 1 and 2 is the result for pET-25b after enzyme cutting. Additionally, number 3 is the pET-25b without enzyme cutting. At last, number 4 and 5 is the PKC-OP after PCR. As our experiment moves on, the data of our PKC-OP and pET-25b are 233.2ng/μL and 17.8ng/μL. Lastly, we use the homologous combination to combine them.
Figure 2 E. coil having the desired pET-25b and PKC-OP
The pET25b-PKC-OP was constructed.
The plate shows monoclonals of pET25b-PKC-OP constructs
Figure 3. The result of Apa1 enzyme digestion validation
Column “marker” is a column that is used to show the position of different lengths of genes. Number 1 to 10 is the result for recombinant plasmid pET25b-PKC-OP after Apa1 enzyme digestion. We get two bands of 4712bp and 1894bp. It further indicates that the obtained monoclonals were positive monoclonals containing the recombinant plasmid.
Future plan: Testing the effectiveness of producing cellulase of positive recombinant bacteria. This is important because we need to know how much bacteria is needed to be used to effectively help animals digest fodder. In this experiment, we can control the time that positive recombinant bacteria grow and use the OD test to set the numbers of bacteria. Then put different numbers of bacteria and cellulose filter paper together. OD test can be used again to see the glucose concentration.
We designed several different induction conditions for protein expression. As the vector we chose to construct the plasmid contains a pelB signal peptide, the engineered strain would secret the protein into the medium. Therefore, we collected the culture supernatant after induction and ran SDS-PAGE for verification. According to the calculation by Snapgene, the expected protein molecular shall weigh 45.6 KDa. As seen from the SDS-PAGE map (Fig. 4), there is a wide band just above 40 KDa and it indicates that we have obtained the PKC-OP protein.
Future Plan
Testing the effectiveness of producing cellulase of positive recombinant bacteria. This is important because we need to know how much bacteria is needed to be used to effectively help animals digest fodder. In this experiment, we can control the time that positive recombinant bacteria grow and use the OD test to set the numbers of bacteria. Then put different numbers of bacteria and cellulose filter paper together. OD test can be used again to see the glucose concentration.
CONTACT INFO

Email: igemteamceres@gmail.com

WeChat Official Account: iGEM Ceres