Team:Shanghai Metro HS/Attributions

Shanghai_Metro_HS

Plasmid Construction:

PCR(polymerase chain reaction)------ Susie & Crystal & Fukuga & Jennifer

Restriction enzyme cutting------ Mark & Miya & Lucy

Recombinant plasmid construction------- Mark & Susie & Crystal

Transformation of the recombinant plasmid into the bacteria----- Lucy & Fukuga & Miya

BL21 Competent Cell:

Standardization of ampicillin solution------- Crystal & Fukuga & Miya

Preparation of culture mediums-------- Lucy & Fukuga & Mark

Culture medium bacteria growth-------- Mark & Susie & Lucy

Bacteria shaking process-------- Mark & Crystal & Susie

Alkaline lysis-------- Lucy & Miya & Mark

Function Test:

DNA electrophoresis--------- Miya & Susie & Mark

DNA electrophoresis gel preparation-------- Lucy & Miya & Fukuga

Protein electrophoresis--------- Crystal & Susie & Miya

Testing of OD540 value(nanodrop)---------- Lucy & Crystal & Mark

Wiki working--------Susie & Crystal & Fukuga & Nina & Jennifer

WeChat Official Account articles writing & page design-------- Fukuga & Susie & Miya