Difference between revisions of "Team:GreatBay SCIE/Dissociation constant"

Revision as of 09:14, 18 October 2021

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With the data, we can simply calculate the kd value by using following equation.

$$\ce{A + T<-->T[K_{on}][K_{off}]AT}$$

$$k_{on}[A][T]=K_{off}[AT]$$

$$K_A= \frac{1}{K_D}=\frac{K_{on}}{K_{off}}=\frac{[AT]}{[A][T]}$$

By using the data, we can compare our affinity with the others' to ensure our binding affinity is outstanding.

Besides we used the equation

$$V=V_{max}\frac{[P]}{K_d\times{[P]}}+M\times[P]$$

As we know the aptamer has the ability to bind with non-specific region.

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 <p>With the data, we can simply calculate the kd value by using following equation.</p>