Team:Paris Bettencourt/Notebook

Collaboration

Notebook & Protocols

Protocols
  • 5' Phosphorylation of primers with T4 PNK


  • Colony PCR


  • Competent cells with CaCl2


  • CPEC ligation


  • CRISPR no Scar


  • DAPI staining for flow cytometry


  • Dpn1 digestion


  • Flow Cytometry sample preparation


  • Fluorescence microscopy


  • Glycerol stocks


  • High throughput transformation


  • M9 media recipe


  • Minicells purification


  • P1 transduction


  • PCR amplification with Q5


  • Pigment production and purification


  • Removal of antibiotic cassette with pCP20


  • Timecourse in 384well plate for fluorescence


  • Transformation via electroporation


  • Taq polymerase
    • Notebook
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    Integrated DNA Technologies
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