Team:NCTU Formosa/Partnership
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Overview
Throughout this year, we collaborate with NTHU on a set of shared objectives related to both of our projects. This partnership is both vital to our project, without each other's help, we wouldn't complete our projects smoothly. In the partnership, we help NTHU in culturing the callus of Pinus armandii. On the other hand, NTHU helps us in the measurement of the O.D. value of the E. coli and the excitation/emission wavelength of RFP and GFP.
See the NTHU_Taiwan team's wiki for more information!March 2021
We have our first meet-up with NTHU on March 26. We discussed our project and thought of the possibility of collaboration. We found out that there are many chances for us to develop our projects together and decided to build up the partnership.
April to July
Because of the Covid-19 pandemic outbreak, we can only have our discussion online through communication software. During this time, we kept on discussing the projects together and giving each other advice. NTHU team members also discussed their culturing experiment design with our instructor Prof. Chen, Wen-Liang since he is the authority in plant culturing.
August
We have our first online meet-up on August 22. Besides exchanging ideas with each other, we ask NTHU if they can help us with collecting and analyzing their school's stary dogs' saliva, which can provide the condition to test our dental bone product. However, due to the difficulty of analyzing each component precisely in saliva, we changed our proposal to NTHU into culturing the Komagataeibacter xylinus which can produce konjac, the main composition of our dental bone design.(Fig.2)
September
NCTU_Formosa → NTHU_Taiwan
2021/09/24-2021/10/30
We help NTHU_Taiwan on culturing the callus of Pinus armandii.
Introduction:
NTHU_Taiwan needs the callus of Pinus armandii to test if their target gene was successfully transferred into plant cells and if it worked. NTHU_Taiwan got all the experimental material and seeds, except for the plant growth chamber. Because the two schools are adjacent, We generously lent them the plant growth chamber from our lab and allowed NTHU_Taiwan to conduct experiments there. Our teachers and members had plentiful experiments on culturing plant callus, we also gave them some advice about how to culture plant callus. The following figures show the course of events. (Fig.4~6 )
Result:
NTHU_Taiwan went here to passage their callus every week and successfully got the plant callus. Pictures below are the growth records for the first week. In the first week, the callus grew slowly. From the second week, we can see the callus growing bigger (Fig.9). Eventually, NTHU_Taiwan use the callus, which NCTU_Formosa cultivated for us in our experiments!
October
The second meet-up is held on October 8 to do further discussion and confirm the progress of the partnership. Because of the covid-19 outbreak, the Food Industry Research and Development Institute in Taiwan cannot deliver NCTU_Formosa's order of Komagataeibacter xylinus in time. Thus, we decided to change our collaboration of culturing Komagataeibacter xylinus to the measurement for model validation.
We wanted to confirm whether the model they designed conformed to the actual situation, thus, we need an extra set of data in the model to be more referential. Although, the limit of time and lack of facility cause difficulties in execution. Moreover, NTHU got the spectrophotometer in their lab and can execute the measurement every hour automatically, which saved much time. So they decided to help us measure whether the O.D value of the growth model fits the prediction of the model we designed.
NTHU_Taiwan → NCTU_Formosa
2021/10/15-2021/10/16
NTHU_Taiwan Help us on the measurement of O.D value of E.coli and the relative fluorescence of RFP and GFP.
Introduction:
We wanted to verify whether the models we designed for predicting the growth of E.coli and its expression conform to the experiment data. We need to measure the O.D value of E.coli and the relative fluorescence of RFP and GFP. To obtain intensive data to compare with the simulated growth curve, E.coli solution needs to be measured an hour at a time in the time span of 24 hours. However, we have no machine that can regularly measure these three values. To solve this problem, NTHU_Taiwan used the machine in their laboratory to assist us in obtaining these values for our model validation. The following are photos of our team went to their laboratory.(Fig.11~13)
Result:
NTHU_Taiwan sent us the result after their measurement.(Fig.14~16) As you can see below, the O.D. value of E. coli had not changed significantly. It showed that E. coli had reached a growth balance. And we could find both the expression of GFP and RFP values were stay in a range, indicating that DenTeeth could stably produce protein.
Because of their assistance, we ensured the stable expression of E. coli and had enough data to compare with the simulation of our model. We also adjusted the parameters to complete the model validation. With this modified model, we can more accurately predict the growth and expression of DenTeeth.
