Team:NCTU Formosa/Notebook
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July 1
None
July 2
None
July 3
None
July 4
None
July 5
None
July 6
None
July 7
None
July 8
None
July 9
None
July 10
None
July 11
None
July 12
None
July 13
None
July 14
None
July 15
None
July 16
None
July 17
None
July 18
None
July 19
None
July 20
None
July 21
None
July 22
None
July 23
None
July 24
None
July 25
None
July 26
None
July 27
None
July 28
None
July 29
Cloning of Sequence A & Sequence B
July 30
Transformation
July 31
None
August 1
Transformation
- Sample:
- plasmid with ccdB into DH5α
- plasmid with yafQ into DH5α
- plasmid with ydfD into DH5α
- plasmid with hipA into DH5α
Taq PCR & gel electrophoresis
- Sample:LuxI-LuxR, mCherry, ydfD
Start training AI
August 2
None
August 3
Alkaline Lysis & Silica Resin
Cloning of Sequence Test 1
August 4
Digest of Sequence Test 1 & pSB1K3
August 5
Gel electrophoresis & Transformation
August 6
Taq PCR
August 7
None
August 8
None
August 9
Digest of Sequence A & Sequence B & pSB1A3
August 10
Cloning of Sequence Test 2
August 11
Cloning of Sequence C
Digest of Sequence A & Sequence B & pSB1K3
August 12
Gel electrophoresis
August 13
Ligation of sequence A & Sequence B & pSB1K3
Ligation of Sequence Test 2 & pSB1K3
August 14
None
August 15
None
August 16
Digest of Sequence C & pSB1K3-2
August 17
SequenceAB
Seq Test 2
August 18
None
August 19
Digest of Seq A/B/pSB1K3 checking
August 20
Gel electrophoresis
August 21
None
August 22
None
August 23
Phusion PCR of gblock fragment Sequence A & Sequence B & Sequence C
August 24
Culturing
August 25
Ligation of Seq A & pSB1K3
August 26
Seq A/pSB1K3 Transformation
August 27
Gel electrophoresis
August 28
None
August 29
None
August 30
Ligaiton of SeqA/pSB1K3
August 31
Transformation of DH5α/pSB1K3/SeqA
September 1
Taq PCR of Seq A
September 2
Gel electrophoresis ; Culturing ; Taq PCR
September 3
None
September 4
None
September 5
None
September 6
Gel electrophoresis ; Culturing
September 7
Cloning of Seq A/B/C
September 8
Reaction mix of plasmid checking
September 9
None
September 10
Digest of Sequence A/B/C
September 11
None
September 12
None
September 13
None
September 14
None
September 15
Digest of SeqA
Gel electrophoresis
Gel extraction
Ligation
Transformation
Culturing
September 16
Seq Test 1 Culturing
Plasmid extraction
Digest
Gel electrophoresis
sequence B Phusion PCR
Seq A/C/SeqTest1&2 sequencing
September 17
Gel electrophoresis
Culturing
September 18
Plasmid extraction
September 19
Digest of seqA
September 20
Gel electrophoresis
Liquid culturing
September 21
Phusion PCR, digestion of seqA
Colony PCR of seqB
SeqTest1 plasmid extraction gel electrophoresis
September 22
Digestion of SeqTest1
gel electrophoresis
Ligation of seqA
September 23
Transformation of seqA and seqTest2
September 24
Culturing seqA
Colony PCR of seqA
September 25
Liquid culture of seqA
Gel electrophoresis
September 26
Plasmid extraction of seqA
Digestion of seqA
September 27
Phusion PCR of seqC
September 28
Digestion of seqC
Gel electrophoresis
September 29
Digestion of seqC
September 30
Gel electrophoresis
Gel extraction
Transformation of SeqA and SeqC
October 1
Colony PCR and culturing of SeqA + SeqC
October 2
Transformation of SeqTest1
gel electrophoresis
Liquid culture of SeqA + SeqC
October 3
Plasmid extraction of SeqA + SeqC
Gel electrophoresis
Phusion PCR of SeqB
October 4
Digestion of SeqB
Ligaiton of SeqA+SeqB+SeqC
October 5
Colony PCR of SeqB
Transformation of SeqA+SeqB+SeqC
October 6
Colony PCR of SeqA+SeqB+SeqC
Liquid culture of SeqA+SeqB+SeqC
October 7
Gel electrophoresis
October 8
Transformation of SeqA+SeqB+SeqC
October 9
Colony PCR of SeqA+SeqB+SeqC
Liquid culture of SeqA+SeqB+SeqC
Gel electrophoresis
October 10
Plasmid extraction of SeqA+SeqB+SeqC
Digestion of SeqA+SeqB+SeqC
October 11
Gel electrophoresis
October 12
Culture SeqA+SeqB+SeqC
October 13
Gel electrophoresis
October 14
Cell lysis of SeqA+SeqB+SeqC
LL-37 functional test
Transformation of SeqA+SeqB+SeqC
October 15
Gel electrophoresis
Colony PCR of SeqA+SeqB+SeqC
October 16
Biosafety experiment
Gel electrophoresis
October 17
Gel electrophoresis
STATH functional test
October 18
LL-37 functional test
Gel electrophoresis
October 19
Measurement the expression of GFP and RFP
October 20
Safety experiment
October 21
Measurement the expression of GFP and RFP