Dr. Pei-Hong Chen
Dr. Chen is a biology teacher at Mingdao High School teaching molecular biology and genetic engineering. He has been promoting and leading iGEM since 2014. As a PI, he taught the right attitude in laboratory safety training and general skills in a molecular biology lab. We thank him for everything this year in iGEM competition, in which he was always accompanying us in brainstorming, designing, performing and trouble-shooting experiments.
Miss Hui-Chuan Hsieh
Teacher Hsieh is a biology teacher at Mingdao High School with expertise in protein engineering and expression/purification systems. She is our second PI who is in charge of the school affairs for the whole process of iGEM competition in our school. She helped us build up an in vitro transcription-translation (TXTL) system. In addition, she went with us to visit professors and experts in the field of bacteriophage and pathogen diagnosis around Taiwan. We thank her for giving precious advice about doing research studies and our iGEM project.
Prof. Ming-Shou Jan
Prof. Jan is a professor at Department of Microbiology and Immunology of Chung Shan Medical University. He had provided lab space at Biosafety Level 2 for Mingdao iGEM teams for many years. We greatly thank him and his doctoral students for being our supervisors in performing Salmonella related experiments in a P2 lab.
Prof. Cheng-Yang Huang
Prof. Huang is a professor at Department of Biomedical Sciences of Chung Shan Medical University. He had provided Salmonella enterica serovar Typhimurium LT2 and guided us how to manipulate a bacterium at Biosafety Level 2. We greatly thank him for safety concerns and comments in developing a Salmonella phage detector.
Prof. Chih-Hsin Hung
Prof. Hung is a professor at Department of Chemical Engineering of I-Shou University. He had provided phages and taught us how to isolate phages against a specific bacterial strain in nature. He helped us build up a phage engineering system in our lab and guided us setting up standard protocols to deal with phages. We greatly thank him for his enthusiasm to educate high school students and his invaluable experience in phage research.
Dr. Cheng-Yi Huang
After discussion with the engineer Huang, Cheng-Yi who works at the FabLab in Mingdao High School, he helped us specifically for the hardware design. He guided us through the 3-D printing process and taught us relevant skills. We greatly thank him for his devotion in our project.
Next, we would like to thank all of the members who have worked on the experiments, without them, our project would never have been this successful.
|Research||Eric S, Yi L, Chen-Hsuan F|
|Designing and Cloning DNA||Yi L, Chen-Hsuan F|
|Overall experiment||Yu-Shiuan L, Yi-Yuan L, Yi L, Chen-Hsuan F, Yu-Lun C|
|Rolling Circle Amplification||Chen-Hsuan F, Yi L|
|Wiki text||Yi-Ting C|
Dry lab includes working on modeling, hardware, and the construction of Wiki page, three of the most important tasks. With a limited number of people, they were still able to carry out high quality works.
|Modeling Research||Hong-Lin W, Yi L|
|Data Analysis||Hong-Lin W|
|Hardware||Yen Kang H, Hong-Lin W||Wiki Page||Min-Hong S, Tzu-Yu W, Chung-Wei C, Yi L|
|Wiki Text||Hong-Lin W, Min-Hong S, Tzu-Yu W|
The wide variety of jobs the Human Practices group has to deal with was in fact overwhelming, we would like to thank them for always sticking around, providing everything they have and making up for the completeness of the project.
|Promotional Video||Yi L, Yu-Shiuan L, Chen-Hsuan F, Yi-Han C, Yun-Chiao H, Tzu-Yu W, Hung-Chieh T,|
|Presentation Video||Yi-Han C, Yun-Chiao H, Yi L, Chen-Hsuan F, Hung-Chieh T, Hong-Lin W|
|Public Relations||Syuan-He W, Yu-Zheng S, Zih-Yun L|
|Public survey design and analysis||Syuan-He W, Yi-Han C|
|Material Design (education)||Syuan-He W, Yi-Han C, Yun-Chiao H, Yu-Jie H, Min-Lin H|
|Local School Education||Syuan-He W, Yi-Han C, Yun-Chiao H, Yi L, Chen-Hsuan F,|
|Interview with Dr. Steven Hagens||Syuan-He W, Chen-Hsuan F, Yi L|
|Interview with Prof. Chih-Hsin Hung||Chen-Hsuan F, Yu-Shiuan L, Yi-Ting C|
|Workshop with Creative Microbiologicals||Yi L, Chen-Hsuan F,|
|Workshop with SuperLab||Yi-Han C, Yun-Chiao H|
|Ho Fang Chicken Ranch visit||Yi L, Yi-Han C, Hung-Chieh T, Chen-Hsuan F,|
|Government lab visit||Yi L, Yun-Chiao H, Yi-Tian C|
|Marketing Plan||Hung-Chieh T|
|Collaborations with other iGEM Teams||Syuan-He W, Yi-Han C, Chih-Ting H|
|Graphic Design||Syuan-He W, Zih-Yun L, Chia-Ni T|
|Wiki Text||Syuan-He W, Yi-Ting C, Yu-Jie H, Min-Lin H, Guan-Yiing H, Chih-Ting H, Yi-Tian C, Chia-Yang C|
Experts for Salmonella Testing
Creative Microbiologicals (Qixin), Ltd.
We would like to thank Creative Microbiologicals for joining our collaborative workshop. During that experience, experts from the company introduced us to their current ways of Salmonella detection and provided some feedback on our method of detecting Salmonella. Moreover, they also taught us about the current regulation and laws regarding food security in our country so that we could have a better understanding of our market.
Ho Fong Chicken Ranch
We would like to appreciate Ho Fong Chicken Ranch for giving us the opportunity to visit their chicken farm and understand the production process of eggs and most importantly, the testing process of eggs. We received lots of invaluable information on food safety, and it was after that trip that we finally decided to tackle the problem of Salmonella.
SuperLab is the third party food inspection agency that collaborates with the government quite often. They accepted our request for a real life workshop at our school when we were still uncertain what to do; they came and lectured us on how they inspect Salmonella on food, the equipment they used, the processes undertaken… etc. Most importantly, they mentioned about the difficulty of the process-- the process usually takes up to a week and requires many kinds of laboratory materials and culture media (for nourishment, selection, identification). This inspired us to target the inefficiency of the process. We can’t thank them enough.
Food and Drug Administration, MOHW of Taichung city
The Food and Drug Administration of Taichung city, granted us an opportunity to visit the national lab used for food safety tests. It gave our team new insights on how the industry operates. They also introduced us to all of the regulations on dairy products (since our targeted problem appears on multiple dairy foods), which helped us make sure that our product is applicable to the actual market. Having a chance to get a sneak peak in a national laboratory is once-in-a-lifetime, we really appreciate the Food and Drug Administration of Taichung for this opportunity.
Advisory From Biotech Companies & iGEM Teams
In an interview we had with Dr. Steven Hagens, the Chief Scientific Officer of Micreos, who had lots of experiences dealing with phage modification, he guided us through the usual process of phage modification and gave us some tips to better infect a phage with DNA. We also introduced our idea to him, and were lucky enough to receive remarkable feedback from such an experienced professional, in which he pointed out one of the major flaws in our first plan-- GFP’s lack of ability to transmit and magnify signals (their effect would be unnoticeable on the eggs).
Special thanks to TAS_Taipei 2020, who heavily inspired us with their approach to their experiment last year--Rolling Circle Amplification. They were also kind enough to pass down their past experiences dealing with this technique.
It provided free licenses to SnapGene Viewer that is a revolutionary software helping us analyze DNA sequences and create vector maps.
Integrated DNA Technologies, Inc.
It provided us with free synthesized genes in our project so as to make our imagination into reality.
It also gave us free synthesized DNA fragments in our project so as to make our imagination into reality.
We requested plasmid DNAs from the non-profit plasmid repository platform, where we thank Dr. Elly Tanaka for his valuable research on Tol2 transposon system and the related plasmids.