iGEM 2021: Mingdao

Mingdao

ATTRIBUTIONS

Members

Instructor

Dr. Pei-Hong Chen

Dr. Chen is a biology teacher at Mingdao High School teaching molecular biology and genetic engineering. He has been promoting and leading iGEM since 2014. As a PI, he taught the right attitude in laboratory safety training and general skills in a molecular biology lab. We thank him for everything this year in iGEM competition, in which he was always accompanying us in brainstorming, designing, performing and trouble-shooting experiments.

Miss Hui-Chuan Hsieh

Teacher Hsieh is a biology teacher at Mingdao High School with expertise in protein engineering and expression/purification systems. She is our second PI who is in charge of the school affairs for the whole process of iGEM competition in our school. She helped us build up an in vitro transcription-translation (TXTL) system. In addition, she went with us to visit professors and experts in the field of bacteriophage and pathogen diagnosis around Taiwan. We thank her for giving precious advice about doing research studies and our iGEM project.

Advisor

Prof. Ming-Shou Jan

Prof. Jan is a professor at Department of Microbiology and Immunology of Chung Shan Medical University. He had provided lab space at Biosafety Level 2 for Mingdao iGEM teams for many years. We greatly thank him and his doctoral students for being our supervisors in performing Salmonella related experiments in a P2 lab.

Prof. Cheng-Yang Huang

Prof. Huang is a professor at Department of Biomedical Sciences of Chung Shan Medical University. He had provided Salmonella enterica serovar Typhimurium LT2 and guided us how to manipulate a bacterium at Biosafety Level 2. We greatly thank him for safety concerns and comments in developing a Salmonella phage detector.

Prof. Chih-Hsin Hung

Prof. Hung is a professor at Department of Chemical Engineering of I-Shou University. He had provided phages and taught us how to isolate phages against a specific bacterial strain in nature. He helped us build up a phage engineering system in our lab and guided us setting up standard protocols to deal with phages. We greatly thank him for his enthusiasm to educate high school students and his invaluable experience in phage research.

Dr. Cheng-Yi Huang

After discussion with the engineer Huang, Cheng-Yi who works at the FabLab in Mingdao High School, he helped us specifically for the hardware design. He guided us through the 3-D printing process and taught us relevant skills. We greatly thank him for his devotion in our project.

Wet lab

Next, we would like to thank all of the members who have worked on the experiments, without them, our project would never have been this successful.

Research	Eric S, Yi L, Chen-Hsuan F
Designing and Cloning DNA	Yi L, Chen-Hsuan F
Overall experiment	Yu-Shiuan L, Yi-Yuan L, Yi L, Chen-Hsuan F, Yu-Lun C
Rolling Circle Amplification	Chen-Hsuan F, Yi L
Wiki text	Yi-Ting C

Dry lab

Dry lab includes working on modeling, hardware, and the construction of Wiki page, three of the most important tasks. With a limited number of people, they were still able to carry out high quality works.

Modeling Research	Hong-Lin W, Yi L
Data Analysis	Hong-Lin W
Hardware	Yen Kang H, Hong-Lin W
Wiki Page	Min-Hong S, Tzu-Yu W, Chung-Wei C, Yi L
Wiki Text	Hong-Lin W, Min-Hong S, Tzu-Yu W

Human Practices

The wide variety of jobs the Human Practices group has to deal with was in fact overwhelming, we would like to thank them for always sticking around, providing everything they have and making up for the completeness of the project.

Promotional Video	Yi L, Yu-Shiuan L, Chen-Hsuan F, Yi-Han C, Yun-Chiao H, Tzu-Yu W, Hung-Chieh T,
Presentation Video	Yi-Han C, Yun-Chiao H, Yi L, Chen-Hsuan F, Hung-Chieh T, Hong-Lin W
Public Relations	Syuan-He W, Yu-Zheng S, Zih-Yun L
Public survey design and analysis	Syuan-He W, Yi-Han C
Material Design (education)	Syuan-He W, Yi-Han C, Yun-Chiao H, Yu-Jie H, Min-Lin H
Local School Education	Syuan-He W, Yi-Han C, Yun-Chiao H, Yi L, Chen-Hsuan F,
Interview with Dr. Steven Hagens	Syuan-He W, Chen-Hsuan F, Yi L
Interview with Prof. Chih-Hsin Hung	Chen-Hsuan F, Yu-Shiuan L, Yi-Ting C
Workshop with Creative Microbiologicals	Yi L, Chen-Hsuan F,
Workshop with SuperLab	Yi-Han C, Yun-Chiao H
Ho Fang Chicken Ranch visit	Yi L, Yi-Han C, Hung-Chieh T, Chen-Hsuan F,
Government lab visit	Yi L, Yun-Chiao H, Yi-Tian C
Marketing Plan	Hung-Chieh T
Collaborations with other iGEM Teams	Syuan-He W, Yi-Han C, Chih-Ting H
Graphic Design	Syuan-He W, Zih-Yun L, Chia-Ni T
Wiki Text	Syuan-He W, Yi-Ting C, Yu-Jie H, Min-Lin H, Guan-Yiing H, Chih-Ting H, Yi-Tian C, Chia-Yang C

Acknowledgements

Experts for Salmonella Testing

Creative Microbiologicals (Qixin), Ltd.

We would like to thank Creative Microbiologicals for joining our collaborative workshop. During that experience, experts from the company introduced us to their current ways of Salmonella detection and provided some feedback on our method of detecting Salmonella. Moreover, they also taught us about the current regulation and laws regarding food security in our country so that we could have a better understanding of our market.

Ho Fong Chicken Ranch

We would like to appreciate Ho Fong Chicken Ranch for giving us the opportunity to visit their chicken farm and understand the production process of eggs and most importantly, the testing process of eggs. We received lots of invaluable information on food safety, and it was after that trip that we finally decided to tackle the problem of Salmonella.

SuperLab (Taimei)

SuperLab is the third party food inspection agency that collaborates with the government quite often. They accepted our request for a real life workshop at our school when we were still uncertain what to do; they came and lectured us on how they inspect Salmonella on food, the equipment they used, the processes undertaken… etc. Most importantly, they mentioned about the difficulty of the process-- the process usually takes up to a week and requires many kinds of laboratory materials and culture media (for nourishment, selection, identification). This inspired us to target the inefficiency of the process. We can’t thank them enough.

Food and Drug Administration, MOHW of Taichung city

The Food and Drug Administration of Taichung city, granted us an opportunity to visit the national lab used for food safety tests. It gave our team new insights on how the industry operates. They also introduced us to all of the regulations on dairy products (since our targeted problem appears on multiple dairy foods), which helped us make sure that our product is applicable to the actual market. Having a chance to get a sneak peak in a national laboratory is once-in-a-lifetime, we really appreciate the Food and Drug Administration of Taichung for this opportunity.

Advisory From Biotech Companies & iGEM Teams

Micreos

In an interview we had with Dr. Steven Hagens, the Chief Scientific Officer of Micreos, who had lots of experiences dealing with phage modification, he guided us through the usual process of phage modification and gave us some tips to better infect a phage with DNA. We also introduced our idea to him, and were lucky enough to receive remarkable feedback from such an experienced professional, in which he pointed out one of the major flaws in our first plan-- GFP’s lack of ability to transmit and magnify signals (their effect would be unnoticeable on the eggs).

TAS_Taipei 2020

Special thanks to TAS_Taipei 2020, who heavily inspired us with their approach to their experiment last year--Rolling Circle Amplification. They were also kind enough to pass down their past experiences dealing with this technique.

Team:Mingdao/Attributions