Team:Mingdao/Calendar

iGEM 2021: Mingdao

CALENDAR

Human Practices
Education

Timeline (2020)

09/18

  Meeting with Gem Mont Biotech Company

10/24

  Bioman training（Education）

10/31

  Science Festival at National Museum of Natural Science

11/7

  Bioman training（Education）

11/21

  Bioman training（Education）

12/28 ~ 12/31

  Steam Fair

12/31

  Mingdao IGEM 2020 Presentation


Timeline (2021)

01/05

  Meeting with Professor Chih-Hsin Hung at I-Shou University

02/23

  Phage-hunting

02/25

  Salmonella phage identification

02/27

  Phage amplification

03/01

  Salmonella phage titration

03/12

  Cloning: TPase-HisN/pSB1C3

03/16

  Meeting with Professor Guo-Jen Wang at National Chung Hsing University

03/26

  Cloning: J04450/pSBII-SK-mTol2

04/10

  Test T7-His-phi29 DNA polymerase fro team TAS_Taipei

04/12

  Cloning of ldhp-GFP-Tr/pBSII-SK-mTol2

  Protein purification: His-phi29 DNA polymerase

04/27

  Meeting with Creative LifeSciences Company

05/06

  Cloning: ldhp-KanR-Tr/pBSII-SK-mTol2

05/19

  TXTL: reagent preparation

  Cloning : ldhp-Phi29-HisN/pBSII-SK-mTol2

06/01

  Meeting with TAS IGEM 2021

06/04

  Cloning : ldhp-RFP, amilCP/pBSII-SK-mTol2

06/08

  Planning modelling version 1: attempt to model optimal waiting time during product usage through the mean of computer simulation of bacteria-phage interaction

06/11

  Found simulation program eVivaldi by researchers. Plan to renew and remake so that it is appropriate for reaching our goal.

06/12

  Meeting with KCIS IGEM 2021

06/15

  Start to design Ni-column

06/18

  Design of Ni-column complete. Ordering materials

06/23

  Renewal of eVivaldi complete, now runs on the newest version of Python. Start to add in new functions and remove unwanted functions, adjusting the program to our needs.

06/24

  Cloning : pSB6K1 & pSB6C1 (pBR322-based)

06/25

  TXTL: in vitro expression of His-TPase (Tol2 transposase)

06/30

  HisPurTM Ni-NTA Resin arrived

07/06

  Cloning :ldhp-GFP-Tr/pSB6A1& pSB6K1 & pSB6C1

  Cloning :Adjustment complete. However, testing shows that result is far from correct. The program’s algorithm also requires input of factors, such as characteristics of phages, that is difficult to measure in our lab

07/9

  First successful Ni-column produced

  Formal meeting of the team deciding whether to change direction. Ultimately decided to get back to mathematical modeling instead of computer simulation. Start to search for theories and researches that will assist in modeling

07/10

  Protein purification: His-TPase

07/19

  Meeting with Micreos CSO – Steven Hagens

07/21

  Start to design the Luer-lock connector

  Found core research paper that helps modeling optimal latent time

07/22

  In vitro plasmid integration assay(1)

07/23

  Meeting with NCKU IGEM 2021

07/25

  In vitro plasmid integration assay(2)

07/26

  Conduct experiment to collect data for future use in modeling

07/27

  In vitro plasmid integration assay(3)

07/30

  Rough design complete, 3D model design starts

08/03

  Briefly organized the equation and logic of the model. Tested using the collected data, but result turned out to be counterintuitive.

08/04

  In vitro phage engineering

  Meeting with Warwick IGEM 2021

08/06

  3D model version 1 complete, test failed. Start to design version 2

08/14

  3D model version 2 complete, adequate yet many small issues exist. Start to design version 3

08/19

  Found a research paper explaining the strange phenomenon. Consider the model as complete.

08/20

  3D model version 3 complete. Passed the test with enough precision. However, the core mechanism breaks after a few tests.

08/21

  Scheduled consultation with 3D printing teacher in school

08/24

  Salmonell test

08/26

  Consultation with a 3D printing teacher in school. Decided to change to photo-curing 3D printing

09/02

  3D model version 3 reprinted with photo-curing 3D printing

09/08

  Testing with Ni-column installed.

09/10

  Finished wiki modeling page content

09/15

  Filming demonstration video

09/20

  Content review complete

09/22

  Demonstration video finished

09/30

  Visiting FDS Taichung

10/12

  Visiting He-Feng chicken farm