Team:GDSYZX/Parts
PARTS
| Name | Type | Description | Length (bp) |
|---|---|---|---|
| BBa_K3880000 | Promoter | pro1380 promoter | 300 |
| BBa_K3880001 | Promoter | pro2953 promoter | 300 |
| BBa_K3880002 | Promoter | pro3035 promoter | 403 |
| BBa_K3880003 | Promoter | pro3040 promoter | 263 |
| BBa_K3880004 | Promoter | pro3071 promoter | 193 |
| BBa_K3880005 | Promoter | pro15290 promoter | 218 |
| BBa_K3880006 | Promoter | pro22610 promoter | 152 |
| BBa_K3880007 | Promoter | pro24880 promoter | 461 |
| BBa_K3880008 | Coding | CDO1(a cysteine dioxygenase) | 573 |
| BBa_K3880009 | Coding | Sep39(protease) | 1161 |
| BBa_K3880010 | Coding | eGFP | 720 |
| BBa_K3880011 | Coding | AtHQT (Hydroxycinnamoyl-CoA Quinate Hydroxycinnamoyl |
1320 |
| BBa_K3880012 | Promoter | ermE promoter | 216 |
| BBa_K3880013 | Promoter | pro05270 promoter | 300 |
1. CDO1
CDO1 encodes a cysteine dioxygenase with 190 amino acids which has been found in Streptomyces sp. SCUT-3. CDO1 could catalyze cysteine’s oxidation to produce sulfite, which is the key factor for feather degradation. The previous study have demonstrated that overexpression of cdo1 increased bacterial single-cell sulfite production and feather protein conversion efficiency obviously.
Co-overexpression of CDO1 and Sep39 under constitutive promoter ermE increased SCUT-3’s disulfide bond reduction and keratin hydrolysis to achieve higher feather degradation efficiency which exhibited highest total peptide and amino acid yields among SCUT-3, SCUT-Osep39 and SCUT-Ocdo1. However, the co-overexpression of CDO1 and Sep39 under this constitutive promoter ermE yielding fewer cells than SCUT-3 and Sep39 overexpression strain (SCUT-Osep39). To further improve the degradation efficiency without influencing the growth rate, we attempt to modified SCUT-3 through replacing the constitutive promoter with well-characterized promoters.
2. Sep39
Sep39 is a major protease involved in SCUT-3’s keratin hydrolysis, and previous work showed that its overexpression could increase SCUT-Osep39’s keratinase activity. To further improve the degradation efficiency without influencing the growth rate, we attempt to balance the expression quantity of CDO1 and Sep39 in SCUT-3 through replacing the constitutive promoter with well-characterized promoters.