During the whole process of our project, collaborations occupy an important position. These efforts enable us to better understand the meaning of our project and recognize what aspects we can improve. Due to the limitations of epidemic, most of our collaborations were held online. Even so, we have gained precious advice and experience from these scientific communications and sharing. We are grateful to have contributed to completing other iGEMers’ projects and to have promoted the communication among synthetic biology communities.
Teams：BNU-China, Cornell, Jiangnan-China, NJTech-China, NNU-China,NWU-China-A, NWU-China-B
On August 14, we hosted the Manufacturing Meetup online. We invited teams from manufacturing track to present their projects, aiming to improve each other’s projects and seek opportunities to establish in-depth cooperation. At the beginning, the professor Zhu, the instructor of BNU-China, presented an excellent speech and talked about the significance of iGEM and the prospect of Synthetic Biology.
During the Meetup, the participant teams had a deep exchange of views on each other's projects and learned a lot from iGEMers’ generous sharing. For example, Jiangnan-China did a really good job in Human Practices, and we got inspired by their experiences. All participants have spent a wonderful time joining the discussion and exchanging ideas freely.
We helped NEU-China to connect the Hrp gene with the vector plasmid pACYCDuet-1. Besides, NEU-China sent us the plasmid pETDuet-1 to complete our project. We have established a good friendship during the period.
We taught BUCT how to determine the consumption of glycero and concentrations of GABA and 5HTP by HPLC, which reflect the production efficiency of their engineering bacteria. However, in their experiments, we noted that the process is really complicated and the result is not very ideal. Therefore, in the following experiments, we adopted the method of measuring the concentrations with the kit.
SCU-China sent plasmid p11-LacY-wtx1 to us and we kept contact during the competition. We also had a brief exchange on primer designing and human practices.
We sent Jianhua Saccharomyces cerevisiae BY4741 and plasmid pML104 and have guided them in the design of project and experiment, etc.
We sent Aspergillus Niger to QHFZ and taught them how to cultivate the strain.
Host: QHFZ, BHSF
Thanks for QHFZ and BHSF’s invitation, we attended the meetup at the scene and made a presentation of our project on June 20. During the meetup, we learned about other teams' projects and made good communication with other iGEMers. The activity has set up the foundation for our frequent communication afterwards. Advisors of the meeting gave us valuable advice on the project’s orientation and recommended some professors in quorum sensing area.
Thanks for BJ101ID’s invitation, we attended the meetup online on June 30. We shared our promotion video and did a presentation related to our project. We also gained some experience in organizing HP from Q&A session..
The Eighth Conference of China iGEMer Community
Host: Conference of China iGEMer Community(CCiC)
On August 27-29, we attended the Eighth Conference of China iGEMer Community (CCiC) online, where we acquired valuable suggestions and inspiration. We have presented our cheater-proof system and have received some useful feedback after the presentation. According to the feedback from judges and other participating teams, we improved our work in experiment, presentation, modeling and human practices. In addition, we should pay more attention on the practical improvements after we got suggestions from surveys. CCiC have provided broad communication opportunities for iGEM teams in China for several years. We hope that the next year, we could have the opportunity to communicate with other iGEMers on the spot.
Thanks for BNUZ-China’s invitation, we attended the Second meeting of the iGEM Intestinal Congress online on September 12. Although our project is not related to intestinal flora directly, we shared our experience during the preparation of the lecture, and the possible application perspective of our project in intestinal flora. In the Q&A session, The BNUZ-China team asked for solutions about how to separate and purify five proteins with similar molecular weights at the same time.We suggested that they could be separated by constructing different specific tags, such as His-tag. We also discussed some experimental problems with other teams.