||AntimCherry-pmrB replaces the extracellular binding domain of the receptor pmrB in the PmrBAC system with an antibody sequence that binds to mCherry, enabling it to open a downstream pathway by binding to mCherry. So we can use this part to make the bacteria respond to the proteins that are linked to mCherry.
||agrB-D is extracted from Staphylococcus aureus and is an important component of the population sensing process. agrB-D expresses two proteins that synthesize AIP, which can stimulate the activity of agrC and is an important link in the intracellular regulatory network.
||sgRNA for T7 promoter
||This sgRNA is used to guide dCas9 to silence the T7 promoter.
||sgRNA for pBAD promoter
||This sgRNA is used to guide dCas9 to silence the pBAD promoter.
||PmrA is the corresponding response regulator (RR) of PmrA/PmrB two-component system of bacteria.
||PmrA-PmrB two-component system can activate and phosphorylates PmrA, which subsequently binds to pmrC promoter and active the expression of downstream.
||ccdB can express an endotoxin that promotes bacterial suicide.
||LacI protein with an LVA degradation tail
||This is a coding region for the LacI protein with an LVA degradation tail and without an RBS.
||As a response regulator in the two-component signal transduction system (TCST) of Staphylococcus aureus, AgrA, which transmits signals to downstream promoter and then regulates genes expression and transcription.
||Membrane protein AgrC, as a signal transduction factor, can be activated by the signal molecule AIP in the external environment, AgrC transfers the phosphate group to AgrA to make it phosphorylation, and the activated AgrA can operate its own function.
||OmpT signal peptide
||As the signal peptide of one of the major outer membrane proteins OmpT, it has the potential to induce the
secretion of certain proteins. It originally has 19 amino acids and two additional amino residues was added to the C-terminus for better cleavage of the signal peptide.
||mCherry is one of several "second-generation" monomeric fluorescent proteins.
||According to the sequence of proinsulin mentioned in the literature, we synthesized a DNA fragment sequence of normal proinsulin.
||Proinsulin with 12 bases changed in the functional zone.
||Proinsulin (Mutation Ⅱ)
||Proinsulin with 8 bases deleted.
||mRNA binding site
||One kind of switches which relies on binding between two complementary mRNAs.
||The yeGFP can express green fluorescence protein, which can be used as a report gene to characterize the strength of promoters.