This year, BNU-China developed a kit based on the cheater-proof system. The kit is designed for users to ultilize the cheater-proof system conveniently and standerdizedly and aims to monitor the stability of target genes in users’ engineered bacteria during microbial production and laboratory research.
By simple restriction and insertion cloning, users can introduce their target genes into our platform to synthesize proteins or carry out further experiments. It is worth noting that users can choose from two types of plasmids according to different mutations of the target gene, assuring of better genetic stability monitoring. When the target gene tends to occur frame shift mutation or other variations leading to expression loss such as gene silencing, please use worker plasmid 2; When the target gene tends to mutate by single-base substitution in the key domain, please use worker plasmid 3. In addition, guard cells will be immobilized which enables themselves to be put in and taken out conveniently. Also, users can evaluate the running condition of our platform by monitoring the expression intensity of mCherry. Moreover, We recommend that you choose the optimal guard ratio based on the probability of degraded bacteria between 10-8 to 10-3 which is predited. (The optimal ratio refers to the ratio of the volume of the guard sepharose beads to the volume of the culture environment.)