![](https://static.igem.org/mediawiki/2021/4/44/T--NCKU_Tainan--paet_header.png)
Overview
BioBricks have always been the foundational building blocks for iGEM projects. When constructing our BioBricks, we made use of the IDT synthesis offer to generate our required parts in a fast and efficient way. Additionally, we made use of PCR amplification with our constructed expression plasmids as a template to construct several BioBricks. All of the parts contain a coding sequence, some of which extended with promoters or introns. We created a part collection comprised of 29 basic parts and 10 improved composite parts. All parts were generated during our project.
Basic parts
Name | Description | Type | Length |
---|---|---|---|
BBa_R0010 | lacI promoter | Promoter | 200bp |
BBa_K156009 | OFP | Coding | 669bp |
BBa_K3071013 | pspA promoter | Promoter | 218bp |
BBa_K3771009 | OmpA/OprF Chimeric Protein | Coding | 1110bp |
BBa_K3771011 | ompA promoter | Promoter | 303bp |
BBa_K3771010 | OmpA | Coding | 1041bp |
BBa_K3771008 | CSAD-6xHis | Coding | 1500bp |
BBa_J23100 | J23100 | Promoter | 35bp |
BBa_J23101 | J23101 | Promoter | 35bp |
BBa_K3771047 | PkatG | Promoter | 45bp |
BBa_K3771048 | PsoxS | Promoter | 85bp |
BBa_K2862010 | PsoxS mutant3 (Imperial) | Promoter | 61bp |
BBa_B0034 | B0034 | RBS | 12bp |
BBa_K2918012 | RiboJ | Regulator | 75bp |
BBa_K223000 | SoxR | Coding | 465bp |
BBa_K1321337 | sfGFP | Coding | 711bp |
BBa_K592012 | RCP | Coding | 681bp |
BBa_K3771012 | CS-6xHis | Coding | 1275bp |
BBa_K3771100 | SoxR (reverse) | Coding | 1275bp |
BBa_K3771000 | JJU | Coding | 465bp |
BBa_K3771006 | JJU-6xHis | Coding | 1191bp |
BBa_K3771001 | CoaBC | Coding | 1221bp |
BBa_K3771007 | CoaBC-6xHis | Coding | 1239bp |
BBa_K3771004 | CDO1 | Coding | 603bp |
BBa_K3771003 | CSAD | Coding | 1482bp |
BBa_K3771002 | CS | Coding | 1257bp |
BBa_K3771041 | CDO1-6xHis | Coding | 1275bp |
BBa_K864400 | Ptrc | Promoter | 30bp |
BBa_I719005 | PT7 | Promoter | 23bp |
Composite Parts
Name | Description | Type | Length |
---|---|---|---|
BBa_K3771013 | PlacI-OFP | Composite | 1090bp |
BBa_K3771014 | PpspA-OFP | 1008bp | |
BBa_K3771015 | PlacI-OmpA/OprF | 1336bp | |
BBa_K3771016 | PompA-OmpA/OprF | 1439bp | |
BBa_K3771017 | PpspA-OFP-PlacI-OmpA/OprF | 2352bp | |
BBa_K3771018 | PpspA-OFP-PompA-OmpA/OprF | 2455bp | |
BBa_K3771020 | PpspA-JJU10 | ??bp | |
BBa_K3771021 | PpspA-JJU10-6xHis | ??bp | |
BBa_K3771022 | PpspA-CoaBC | ??bp | |
BBa_K3771023 | PpspA-CoaBC-6xHis | ??bp | |
BBa_K3771024 | PpspA-CSAD | ??bp | |
BBa_K3771025 | PpspA-CSAD-6xHis | ??bp | |
BBa_K3771026 | PpspA-JJU10-PlacI-OmpA/OprF | 2661bp | |
BBa_K3771027 | PpspA-JJU10-6xHis-PlacI-OmpA/OprF | 2679bp | |
BBa_K3771028 | PpspA-CoaBC-PlacI-OmpA/OprF | 2709bp | |
BBa_K3771029 | PpspA-CoaBC-6xHis-PlacI-OmpA/OprF | 2727bp | |
BBa_K3771030 | PpspA-CSAD-PlacI-OmpA/OprF | 2970bp | |
BBa_K3771031 | PpspA-CSAD-6xHis-PlacI-OmpA/OprF | 2988bp | |
BBa_K3771032 | PpspA-JJU10-PompA-OmpA/OprF | 2764bp | |
BBa_K3771033 | PpspA-JJU10-6xHis-PompA-OmpA/OprF | 2782bp | |
BBa_K3771034 | PpspA-CoaBC-PompA-OmpA/OprF | 2812bp | |
BBa_K3771035 | PpspA-CoaBC-6xHis-PompA-OmpA/OprF | 2830bp | |
BBa_K3771036 | PpspA-CSAD-PompA-OmpA/OprF | 3073bp | |
BBa_K3771037 | PpspA-CSAD-6xHis-PompA-OmpA/OprF | 3091bp | |
BBa_K3771038 | PpspA-CS | ??bp | |
BBa_K3771039 | PpspA-CS-6xHis | ??bp | |
BBa_K3771040 | PpspA-CDO1 | ??bp | |
BBa_K3771042 | PpspA-CDO1-6xHis | ??bp | |
BBa_K3771043 | PpspA-CS-PompA-OmpA/OprF | ??bp | |
BBa_K3771044 | PpspA-CS-6xHis-PompA-OmpA/OprF | 2212bp | |
BBa_K3771045 | PpspA-CDO1-PompA-OmpA/OprF | 2194bp | |
BBa_K3771046 | PpspA-CDO1-6xHis-PompA-OmpA/OprF | 2212bp | |
BBa_K3771050 | J23100-sfGFP | 774bp | |
BBa_K3771091 | PkatG-sfGFP | 784bp | |
BBa_K3771092 | sam8 with ribosome binding site | 824bp | |
BBa_K3771093 | sam8 with ribosome binding site | 1297bp | |
BBa_K3771094 | sam8 with ribosome binding site | 1386bp | |
BBa_K3771095 | sam8 with ribosome binding site | 1859bp | |
BBa_K3771096 | sam8 with ribosome binding site | 1205bp | |
BBa_K3771097 | sam8 with ribosome binding site | 2084bp | |
BBa_K3771098 | sam8 with ribosome binding site | 1409bp | |
BBa_K3771099 | sam8 with ribosome binding site | 1441bp | |
BBa_K3771075 | sam8 with ribosome binding site | ??bp | |
BBa_K3771076 | sam8 with ribosome binding site | 1688bp | |
BBa_K3771077 | sam8 with ribosome binding site | 1549bp | |
BBa_K3771078 | sam8 with ribosome binding site | 1324bp | |
BBa_K3771079 | sam8 with ribosome binding site | ??bp | |
BBa_K3771080 | sam8 with ribosome binding site | 1250bp |
![](https://static.igem.org/mediawiki/2021/1/18/T--NCKU_Tainan--backtotop.png)