Team:NCKU Tainan/Parts

Overview

  BioBricks have always been the foundational building blocks for iGEM projects. When constructing our BioBricks, we made use of the IDT synthesis offer to generate our required parts in a fast and efficient way. Additionally, we made use of PCR amplification with our constructed expression plasmids as a template to construct several BioBricks. All of the parts contain a coding sequence, some of which extended with promoters or introns. We created a part collection comprised of 29 basic parts and 48 improved composite parts. All parts were generated during our project.

Basic parts

Name Description Type Length
BBa_R0010 lacI promoter Promoter 200bp
BBa_K156009 OFP Coding 669bp
BBa_K3071013 pspA promoter Promoter 218bp
BBa_K3771009 OmpA/OprF Chimeric Protein Coding 1110bp
BBa_K3771011 ompA promoter Promoter 303bp
BBa_K3771010 OmpA Coding 1041bp
BBa_K3771008 CSAD-6xHis Coding 1500bp
BBa_J23100 J23100 Promoter 35bp
BBa_J23101 J23101 Promoter 35bp
BBa_K3771047 PkatG Promoter 45bp
BBa_K3771048 PsoxS Promoter 85bp
BBa_K2862010 PsoxS mutant3 (Imperial) Promoter 61bp
BBa_B0034 B0034 RBS 12bp
BBa_K2918012 RiboJ Regulator 75bp
BBa_K3771100 SoxR Coding 465bp
BBa_K1321337 sfGFP Coding 711bp
BBa_K592012 RCP Coding 681bp
BBa_K3771012 CS-6xHis Coding 1275bp
BBa_K3771100 SoxR (reverse) Coding 1275bp
BBa_K3771000 JJU Coding 465bp
BBa_K3771006 JJU-6xHis Coding 1191bp
BBa_K3771001 CoaBC Coding 1221bp
BBa_K3771007 CoaBC-6xHis Coding 1239bp
BBa_K3771004 CDO1 Coding 603bp
BBa_K3771003 CSAD Coding 1482bp
BBa_K3771002 CS Coding 1257bp
BBa_K3771041 CDO1-6xHis Coding 1275bp
BBa_K864400 Ptrc Promoter 30bp
BBa_I719005 PT7 Promoter 23bp

Composite Parts

Name Description Type Length
BBa_K3771013 PlacI-ofp Composite 1090bp
BBa_K3771014 PpspA-ofp 1008bp
BBa_K3771015 PlacI-ompA/oprF 1336bp
BBa_K3771016 PompA-ompA/oprF 1439bp
BBa_K3771017 PpspA-ofp-PlacI-ompA/oprF 2352bp
BBa_K3771018 PpspA-ofp-PompA-ompA/oprF 2455bp
BBa_K3771020 PpspA-jju 1317bp
BBa_K3771021 PpspA-jju-6xHis 1335bp
BBa_K3771022 PpspA-coaBC 1365bp
BBa_K3771023 PpspA-coaBC-6xHis 1383bp
BBa_K3771024 PpspA-csad 1626bp
BBa_K3771025 PpspA-csad-6xHis 1644bp
BBa_K3771026 PpspA-jju-PlacI-ompA/oprF 2661bp
BBa_K3771027 PpspA-jju-6xHis-PlacI-ompA/oprF 2679bp
BBa_K3771028 PpspA-coaBC-PlacI-ompA/oprF 2709bp
BBa_K3771029 PpspA-coaBC-6xHis-PlacI-ompA/oprF 2727bp
BBa_K3771030 PpspA-csad-PlacI-ompA/oprF 2970bp
BBa_K3771031 PpspA-csad-6xHis-PlacI-ompA/oprF 2988bp
BBa_K3771032 PpspA-jju-PompA-ompA/oprF 2764bp
BBa_K3771033 PpspA-jju-6xHis-PompA-ompA/oprF 2782bp
BBa_K3771034 PpspA-coaBC-PompA-ompA/oprF 2812bp
BBa_K3771035 PpspA-coaBC-6xHis-PompA-ompA/oprF 2830bp
BBa_K3771036 PpspA-csad-PompA-ompA/oprF 3073bp
BBa_K3771037 PpspA-csad-6xHis-PompA-ompA/oprF 3091bp
BBa_K3771038 PpspA-cs 1401bp
BBa_K3771039 PpspA-cs-6xHis 1419bp
BBa_K3771040 PpspA-cdo1 747bp
BBa_K3771042 PpspA-cdo1-6xHis 765bp
BBa_K3771043 PpspA-cs-PompA-ompA/oprF 2848bp
BBa_K3771044 PpspA-cs-6xHis-PompA-ompA/oprF 2212bp
BBa_K3771045 PpspA-cdo1-PompA-ompA/oprF 2194bp
BBa_K3771046 PpspA-cdo1-6xHis-PompA-ompA/oprF 2212bp
BBa_K3771050 J23100-sfgfp 774bp
BBa_K3771091 PkatG-sfgfp 784bp
BBa_K3771092 PsoxS-sfgfp 824bp
BBa_K3771093 soxR-PsoxS-sfgfp 1297bp
BBa_K3771094 PsoxS-cs-6xHis 1386bp
BBa_K3771095 soxR-PsoxS-cs-6xHis 1859bp
BBa_K3771096 soxR-PsoxS-cdo1-6xHis 1205bp
BBa_K3771097 soxR-PsoxS-csad-6xHis 2084bp
BBa_K3771098 PsoxS-RiboJ-rcp-J23101-soxR 1409bp
BBa_K3771099 PsoxS-RiboJ-sfgfp-J23101-soxR 1441bp
BBa_K3771075 Ptrc-cdo1 690bp
BBa_K3771076 PlacI-csad 1708bp
BBa_K3771077 Ptrc-csad 1569bp
BBa_K3771078 Ptrc-cs 1344bp
BBa_K3771079 PT7-jju 1222bp
BBa_K3771080 PT7-coaBC 1270bp

Parts Collection

Function Name Description Type Length
Detection of Depressive Symptoms BBa_K3771048 PsoxS Promoter 85bp
BBa_K3771100 soxR Coding 465bp
BBa_K3771092 PsoxS-sfgfp Composite 824bp
BBa_K3771093 soxR-PsoxS-sfgfp Composite 1297bp
BBa_K3071013 pspA promoter Promoter 118bp
BBa_K156009 OFP Coding 864bp
BBa_K3771009 OmpA/OprF Chimeric Protein Coding 1110bp
BBa_K3771017 PpspA-ofp-PlacI-ompA/oprF Composite 2352bp
BBa_K3771018 PpspA-ofp-PompA-ompA/oprF Composite 2455bp
Taurine production BBa_K3771097 soxR-PsoxS-csad-6xHis Composite 2084bp
BBa_K3771044 PpspA-cs-6xHis-PompA-ompA/oprF Composite 2212bp
BBa_K3771075 Ptrc-cdo1 Composite 690bp
BBa_K3771000 JJU Coding 1173bp
BBa_K3771001 CoaBC Coding 1221bp