Team:NCKU Tainan/Notebook

Collab

Check this page out to see what we did!

Wet

  1. Perform PCR to copy ompA gene from Escherichia coli.

  2. Basic lab skills.

  3. First attempt to make the competent cell of DH5α and BL21.

  4. Design the primer of IDT fragment.

Dry

  1. Design Wiki page structure.

  2. Hardware Brainstorming.

  3. Modeling research and brainstorming about possible modeling topics.

HP

  1. Booklet design.

  2. Meetup with other iGEM teams.

Wet

  1. Culture the competent cell of DH5α and BL21.

  2. Clone ompA into DH5α.

  3. Design the functional test for taurine production.

Dry

  1. Designing the first version of the salivary cortisol detection system.

  2. Searching data available for the Deep Learning system.

  3. Brainstorming the E. coli metabolism system.

HP

  1. Collaboration with other iGEM teams.

  2. Recording ‘Tea Time’ podcast.

Wet

  1. Culture different competent cells for JJU, CoaBC functional test.

  2. Freeze the engineered bacteria.

Dry

  1. Searching for experts regarding salivary cortisol system.

  2. Learning the basics of Deep Learning.

  3. Searching data for E. coli metabolism system.

HP

  1. Collaboration with other iGEM teams.

  2. Expert visits.

Wet

  1. Finalize the primer design.

  2. Design for the interferon-gamma assay.

  3. Establish the protocol of oxidative stress assay.

Dry

  1. Expert visit.

  2. Brainstorm application design and functions.

  3. Determine the color of the wiki.

  4. Searching data for E. coli metabolism system.

HP

  1. iGEM Taiwan Online Conference.

  2. Game design collaboration with iGEM Moscow.

Wet

  1. Establish the protocol of functional tests for taurine production.

  2. Transform plasmid with jju, coaBC gene into bacteria.

  3. Amplify the product we got from IDT synthesized with PCR.

Dry

  1. Pre-process the data for the Deep Learning system.

  2. Search for possible modeling pathways.

  3. Design Wiki layout.

  4. Searching data for E. coli metabolism system.

HP

  1. Collaboration with other iGEM teams.

  2. FITI business competition.

Wet

  1. Double Digest the gene fragment.

  2. Extract the pUC from the plate.

Dry

  1. Begin coding for the Deep Learning system and App.

  2. Abandoned the salivary cortisol detection system due to sudden covid strikes in Taiwan, thus we cannot find any lab to perform our experiment.

  3. Learning the basic knowledge of Michaelis-Menten equations.

  4. Learning the basic knowledge of Flow balance analysis.

  5. Start editing the wiki templates.

HP

  1. Collaboration with other iGEM teams.

  2. Momentizing interview.

Wet

  1. Culture IDT fragments into different bacteria.

  2. Use the three fragment assembly to clone pspA promoter, ompA/oprF, and ofp together on pUC.

  3. Establish the protocol of the biosafety experiment.

Dry

  1. We think that our ability to code is not enough and we are not sure that the end-product will be good enough, so we started brainstorming new hardware ideas and settled with microfluidics.

  2. Learning the basic knowledge of Michaelis-Menten equations.

  3. Learning the basic knowledge of Flow balance analysis.

HP

  1. Collaboration with other iGEM teams.

  2. Designing souvenirs.

Wet

  1. Clone both katG promoter, soxS promoter and sfGFP successfully into DH5α.

  2. Redo the IFN-γ assay with different concentrations.

  3. Run SDS-PAGE for JJU and CoaBC.

Dry

  1. Learning about microfluidics and designing the first design of microfluidics.

  2. Learning the basics of our project’s mechanism.

  3. Figure out the relationship between taurine and oxidative stress.

HP

  1. Collaboration with other iGEM teams.

  2. Designing souvenirs.

  3. Preparing for iASK Symposium.

Wet

  1. Clone both ompA, pspA promoter and also csad into BL21.

  2. Do the HPLC of JJU and CoaBC.

  3. Clone jju, cdo1 represently into DH5α.

Dry

  1. Present our first design to our professor, and found out that the first design had several problems, so we make a new design with the professor’s advice.

  2. Learning COPASI and Python.

HP

  1. Collaboration with other iGEM teams.

  2. Discussing with iGEM SDU Denmark for modeling collaboration.

  3. Preparing for iASK Symposium.

Wet

  1. Run the SDS-PAGE for CSAD.

  2. Do the oxidative stress assay using PQ and H2O2.

  3. Analyze the data of HPLC.

Dry

  1. Finalized our first design and after some verification from our senior, we finally send the design to the manufacturer to make the Photomask.

  2. Utilizing Python & COPASI to modulate our two models.

HP

  1. Collaboration with other iGEM teams.

  2. Chen En online visit.

Wet

  1. Establish the protocol of ompA knockout experiment.

  2. Do the biosafety experiment.

  3. Do the three fragment assembly with cs, cdo1, and csad.

Dry

  1. Received the Photomask, and start to fabricate the microfluidic chip.

  2. Build the prototype of our model 1.

  3. Searching for more feasible parameters.

HP

  1. Collaboration with other iGEM teams.

  2. Conducting market survey.

Wet

  1. Update the newest result from oxidative stress assay.

  2. Improve the protocol in IFN-γ assay.

  3. Freeze current bacteria.

Dry

  1. Start to perform experiments with the microfluidic chip.

  2. Finishing building model 1. Having a meeting with the Wet team.

  3. Optimizing our parameters.

HP

  1. Collaboration with other iGEM teams.

  2. Organizing market survey responses.

Wet

  1. Do bubble experiment with different pH conditions.

  2. Establish the protocol of tauD knockout experiment.

  3. Improve the protocol of HPLC and redo it.

  4. Test CS, CDO1 and CSAD kinetic.

Dry

  1. Changed the experimental procedure of f(int) to make our results better.

  2. Start building Model 2.

  3. Constructing the mechanism.

HP

  1. Collaboration with other iGEM teams.

  2. iASK Symposium.

  3. Designing four-grid short comic.

Wet

  1. Do SDS-PAGE of PsoxS-csad.

  2. Do bubble experiment with different pH conditions and sizes.

  3. Do SDS-PAGE and western blot of PpspA-cs-PompA-ompA/oprF.

Dry

  1. Changed the procedure of bacteria injection to the channel.

  2. Building and debugging Model2.

HP

  1. Collaboration with other iGEM teams.

  2. Writing MenTAUR business plan.

  3. Gather Town study camp preparation.

Wet

  1. Do SDS-PAGE and western blot of PpspA-cdo1-PompA-ompA/oprF.

  2. Improve the protocol of bubble experiment.

  3. Upload the protocols to our wiki page.

  4. Gather Town study camp.

Dry

  1. We accidentally realized that the injection duration of BSA can make a big difference in retention rate result, then we started to experiment with different duration and realize 6 minutes is the best.

  2. Finished building model 2 and had an interview with professionals.

HP

  1. Collaboration with other iGEM teams.

  2. Inquiring TFDA and NARLabs regarding law and safety.

  3. TWBIO visit with iGEM CCU_Taiwan.

Wet

  1. Test the best concentration of IFN-γ to induce pspA promoter.

  2. Upload the wet design to our wiki page.

Dry

  1. We started to design Generation 2, and send the complete design to the manufacturer.

  2. We started to use a microscope with mercury light to see bacterial spread through the channel.

  3. We started to write Wiki.

  4. HP wiki page design.

HP

  1. Collaboration with other iGEM teams.

  2. Gather Town long-term exhibition upload.

Wet

  1. Do HPLC of PpspA-cs-PompA-ompA/oprF, Ptrc-cdo1 and PsoxS-csad.

  2. Discuss the criteria that we have met.

Dry

  1. Generation 2 photomask finished, then we started to fabricate the chip.

  2. Fixing the mistakes of model.

HP

  1. Collaboration with other iGEM teams.

  2. Designing education kit.

Wet

  1. Organize the data.

  2. Finalize our gene design.

  3. Upload the results to our wiki page.

Dry

  1. Completing f(int) Generation 2 data.

  2. Completing fabricating models.

  3. Finish all wiki page design.

HP

  1. Organizing HP content for the wiki.

  2. Joining Undergraduate Research Day.

e-mail : igem.ncku.tainan@gmail.com
phone : 06-2757575