Team:NCKU Tainan/Parts
Overview
BioBricks have always been the foundational building blocks for iGEM projects. When constructing our BioBricks, we made use of the IDT synthesis offer to generate our required parts in a fast and efficient way. Additionally, we made use of PCR amplification with our constructed expression plasmids as a template to construct several BioBricks. All of the parts contain a coding sequence, some of which extended with promoters or introns. We created a part collection comprised of 29 basic parts and 10 improved composite parts. All parts were generated during our project.
Basic parts
| Name | Description | Type | Length |
|---|---|---|---|
| BBa_R0010 | lacI promoter | Promoter | 200bp |
| BBa_K156009 | OFP | Coding | 669bp |
| BBa_K3071013 | pspA promoter | Promoter | 218bp |
| BBa_K3771009 | OmpA/OprF Chimeric Protein | Coding | 1110bp |
| BBa_K3771011 | ompA promoter | Promoter | 303bp |
| BBa_K3771010 | OmpA | Coding | 1041bp |
| BBa_K3771008 | CSAD-6xHis | Coding | 1500bp |
| BBa_J23100 | J23100 | Promoter | 35bp |
| BBa_J23101 | J23101 | Promoter | 35bp |
| BBa_K3771047 | PkatG | Promoter | 45bp |
| BBa_K3771048 | PsoxS | Promoter | 85bp |
| BBa_K2862010 | PsoxS mutant3 (Imperial) | Promoter | 61bp |
| BBa_B0034 | B0034 | RBS | 12bp |
| BBa_K2918012 | RiboJ | Regulator | 75bp |
| BBa_K223000 | SoxR | Coding | 465bp |
| BBa_K1321337 | sfGFP | Coding | 711bp |
| BBa_K592012 | RCP | Coding | 681bp |
| BBa_K3771012 | CS-6xHis | Coding | 1275bp |
| BBa_K3771100 | SoxR (reverse) | Coding | 1275bp |
| BBa_K3771000 | JJU | Coding | 465bp |
| BBa_K3771006 | JJU-6xHis | Coding | 1191bp |
| BBa_K3771001 | CoaBC | Coding | 1221bp |
| BBa_K3771007 | CoaBC-6xHis | Coding | 1239bp |
| BBa_K3771004 | CDO1 | Coding | 603bp |
| BBa_K3771003 | CSAD | Coding | 1482bp |
| BBa_K3771002 | CS | Coding | 1257bp |
| BBa_K3771041 | CDO1-6xHis | Coding | 1275bp |
| BBa_K864400 | Ptrc | Promoter | 30bp |
| BBa_I719005 | PT7 | Promoter | 23bp |
Composite Parts
| Name | Description | Type | Length |
|---|---|---|---|
| BBa_K3771013 | PlacI-OFP | Composite | 1090bp |
| BBa_K3771014 | PpspA-OFP | 1008bp | |
| BBa_K3771015 | PlacI-OmpA/OprF | 1336bp | |
| BBa_K3771016 | PompA-OmpA/OprF | 1439bp | |
| BBa_K3771017 | PpspA-OFP-PlacI-OmpA/OprF | 2352bp | |
| BBa_K3771018 | PpspA-OFP-PompA-OmpA/OprF | 2455bp | |
| BBa_K3771020 | PpspA-JJU10 | ??bp | |
| BBa_K3771021 | PpspA-JJU10-6xHis | ??bp | |
| BBa_K3771022 | PpspA-CoaBC | ??bp | |
| BBa_K3771023 | PpspA-CoaBC-6xHis | ??bp | |
| BBa_K3771024 | PpspA-CSAD | ??bp | |
| BBa_K3771025 | PpspA-CSAD-6xHis | ??bp | |
| BBa_K3771026 | PpspA-JJU10-PlacI-OmpA/OprF | 2661bp | |
| BBa_K3771027 | PpspA-JJU10-6xHis-PlacI-OmpA/OprF | 2679bp | |
| BBa_K3771028 | PpspA-CoaBC-PlacI-OmpA/OprF | 2709bp | |
| BBa_K3771029 | PpspA-CoaBC-6xHis-PlacI-OmpA/OprF | 2727bp | |
| BBa_K3771030 | PpspA-CSAD-PlacI-OmpA/OprF | 2970bp | |
| BBa_K3771031 | PpspA-CSAD-6xHis-PlacI-OmpA/OprF | 2988bp | |
| BBa_K3771032 | PpspA-JJU10-PompA-OmpA/OprF | 2764bp | |
| BBa_K3771033 | PpspA-JJU10-6xHis-PompA-OmpA/OprF | 2782bp | |
| BBa_K3771034 | PpspA-CoaBC-PompA-OmpA/OprF | 2812bp | |
| BBa_K3771035 | PpspA-CoaBC-6xHis-PompA-OmpA/OprF | 2830bp | |
| BBa_K3771036 | PpspA-CSAD-PompA-OmpA/OprF | 3073bp | |
| BBa_K3771037 | PpspA-CSAD-6xHis-PompA-OmpA/OprF | 3091bp | |
| BBa_K3771038 | PpspA-CS | ??bp | |
| BBa_K3771039 | PpspA-CS-6xHis | ??bp | |
| BBa_K3771040 | PpspA-CDO1 | ??bp | |
| BBa_K3771042 | PpspA-CDO1-6xHis | ??bp | |
| BBa_K3771043 | PpspA-CS-PompA-OmpA/OprF | ??bp | |
| BBa_K3771044 | PpspA-CS-6xHis-PompA-OmpA/OprF | 2212bp | |
| BBa_K3771045 | PpspA-CDO1-PompA-OmpA/OprF | 2194bp | |
| BBa_K3771046 | PpspA-CDO1-6xHis-PompA-OmpA/OprF | 2212bp | |
| BBa_K3771050 | J23100-sfGFP | 774bp | |
| BBa_K3771091 | PkatG-sfGFP | 784bp | |
| BBa_K3771092 | sam8 with ribosome binding site | 824bp | |
| BBa_K3771093 | sam8 with ribosome binding site | 1297bp | |
| BBa_K3771094 | sam8 with ribosome binding site | 1386bp | |
| BBa_K3771095 | sam8 with ribosome binding site | 1859bp | |
| BBa_K3771096 | sam8 with ribosome binding site | 1205bp | |
| BBa_K3771097 | sam8 with ribosome binding site | 2084bp | |
| BBa_K3771098 | sam8 with ribosome binding site | 1409bp | |
| BBa_K3771099 | sam8 with ribosome binding site | 1441bp | |
| BBa_K3771075 | sam8 with ribosome binding site | ??bp | |
| BBa_K3771076 | sam8 with ribosome binding site | 1688bp | |
| BBa_K3771077 | sam8 with ribosome binding site | 1549bp | |
| BBa_K3771078 | sam8 with ribosome binding site | 1324bp | |
| BBa_K3771079 | sam8 with ribosome binding site | ??bp | |
| BBa_K3771080 | sam8 with ribosome binding site | 1250bp |
