Overview
BioBricks have always been the foundational building blocks for iGEM projects. When constructing our BioBricks, we made use of the IDT synthesis offer to generate our required parts in a fast and efficient way. Additionally, we made use of PCR amplification with our constructed expression plasmids as a template to construct several BioBricks. All of the parts contain a coding sequence, some of which extended with promoters or introns. We created a part collection comprised of 14 basic parts and 10 improved composite parts. All parts were generated during our project.
Basic parts
Name | Description | Type | Length |
---|---|---|---|
BBa_R0010 | Constitutive promoter (PJ23100) | Promoter | 200bp |
BBa_K156009 | FNR promoter (Pfnr) | Coding | 669bp |
BBa_K3071013 | Tyrosine ammonia-lyase native RBS (NRBS) | Promoter | 218bp |
BBa_K3771009 | Double terminator (B0010-B0010) | Coding | 1110bp |
BBa_K3771011 | Tyrosine transporter (tyrP) | Promoter | 303bp |
BBa_K3771010 | Tyrosine ammonia-lyase (sam8) | Coding | 1041bp |
BBa_K2997001 | Bacteriocin Full length (CBM-B) | Coding | 867bp |
BBa_K2997002 | Bacteriocin short length (sCBM-B) | Coding | 252bp |
BBa_K2997003 | Sigma 54-dependent transcriptional regulator (pchR) | Coding | 1854bp |
BBa_K2997004 | pchR binding range | Coding | 567bp |
BBa_J18921 | 6aa [GS]x linker | Coding | 18bp |
BBa_K1610300 | Secretion tag (yebF) | Coding | 354bp |
BBa_E0040 | Wild type green fluorescence protein (wtgfp) | Coding | 720bp |
BBa_K2762017 | Superfolder green fluorescence protein (sfgfp) | Coding | 720bp |
Composite Parts
Name | Description | Type | Length |
---|---|---|---|
BBa_K2997005 | K880005 - yebF - GS linker - sCBM-B | Composite | 685bp |
BBa_K2997006 | K880005 - yebF- TB linker - sCBM-B | 120bp | |
BBa_K2997007 | K880005 - yebF - CBM-B | 1282bp | |
BBa_K2997008 | Native promoter - pchR - sfgfp | 3137bp | |
BBa_K2997009 | J23100 - NRBS - sam8 | 1606bp | |
BBa_K2997010 | K880005 - sam8 | 1613bp | |
BBa_K2997011 | B0034 - sam8 | 1572bp | |
BBa_K2997012 | Pfnr-B0034-GFP | 866bp | |
BBa_I742106 | sam8 with ribosome binding site | 1550bp | |
BBa_K880005 | Strong promoter J23100 and strong RBS B0034 | Promoter+RBS | 55bp |