Overview

  With quorum sensing and repressible promoter, our biobrick has a time span to achieve oral sterilization and restoration. With the increase of oral bacteria, it will activate the antimicrobial peptide LL-37 to inhibit the large number of bacteria that cause tooth decay and periodontal disease. When the number of bacteria decrease, it will activate BMP2 and STATH to repair gums and help tooth remineralization. This mechanism can avoid the formation of biofilm during the remineralization process, and it also can achieve the best efficient restoration on the condition of fewer bacteria.

DenTeeth

E .coli DenTeeth

  DenTeeth is the revolution of E. coli BL21. Since E. coli BL21 is a colony that has the properties that have the high-level expression of recombinant proteins, we can test our protein function more efficiently.

Expression conditions : Under high temperature

  The RBS of BBa_K3736001 (Seq-A) needs to exceed 37 degrees to enable its mRNA to begin translation and initiate quorum sensing regulation. In this way, we can ensure that our engineered bacteria can operate in a specific temperature range. When it develops into a commodity in the future, it can also reduce the production of antimicrobial peptides before entering the body of a warm-blooded animal, making it stable and easy to store.

Expression adjustment : Quorum sensing

  When the LuxR protein produced by BBa_K3736001 (Seq-A) binds to the AHL produced by all bacteria, it can prompt the Plux promoter of BBa_K3736002 (Seq-B) to start transcription, so that BBa_K3736002 (Seq-B) can be expressed when there are more bacteria. When the tetR protein in BBa_K3736002 (Seq-B) is expressed, the Ptet promoter of BBa_K3736003 (Seq-C) will be inhibited to achieve the antagonistic effect of BBa_K3736002 (Seq-B) and BBa_K3736003 (Seq-C).

LL-37 antimicrobial peptide

  LL-37 antimicrobial peptide is an antimicrobial peptide from humans, to improve its performance, we performed codon harmonization. The optimized sequence of codons will be more suitable for expression by our engineered strains than the natural sequence.