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<section> | <section> | ||
<h2 id ="Subtitle2">Approaches</h2> | <h2 id ="Subtitle2">Approaches</h2> | ||
− | <h3> | + | <h3>Menbles</h3> |
<p>  Menbles are bubbles containing our engineered <i>E. coli Nissle </i> which aims to alleviate CSID by producing taurine when it senses a high concentration of IFN-γ and oxidative stress. As edible bubbles, Menbles are designed to be affordable and highly accessible product that can be easily incorporated into our daily lives, created to be an alternative solution for people who fear seeking medical help due to social stigma and/or low drug compliance<sup>[<a href="#ref9">9</a>,<a href="#ref10">10</a>]</sup>. | <p>  Menbles are bubbles containing our engineered <i>E. coli Nissle </i> which aims to alleviate CSID by producing taurine when it senses a high concentration of IFN-γ and oxidative stress. As edible bubbles, Menbles are designed to be affordable and highly accessible product that can be easily incorporated into our daily lives, created to be an alternative solution for people who fear seeking medical help due to social stigma and/or low drug compliance<sup>[<a href="#ref9">9</a>,<a href="#ref10">10</a>]</sup>. | ||
The main ingredient of Menbles is <b>sodium alginate</b>, which is able to resist degradation in environments with low pH, such as in the stomach gastric acid<sup>[<a href="#ref11">11</a>,<a href="#ref12">12</a>]</sup>. <b>In environments of higher pH such as in the small intestine (pH 7.4), Menbles are able to release our engineered bacteria into its surroundings and initiate the production of taurine.</b></p> | The main ingredient of Menbles is <b>sodium alginate</b>, which is able to resist degradation in environments with low pH, such as in the stomach gastric acid<sup>[<a href="#ref11">11</a>,<a href="#ref12">12</a>]</sup>. <b>In environments of higher pH such as in the small intestine (pH 7.4), Menbles are able to release our engineered bacteria into its surroundings and initiate the production of taurine.</b></p> | ||
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<a href="https://static.igem.org/mediawiki/2021/b/b8/T--NCKU_Tainan--menbleonthespoon.jpg" target="_blank" style="width:50%"><img src="https://static.igem.org/mediawiki/2021/b/b8/T--NCKU_Tainan--menbleonthespoon.jpg" alt="" title="" style="width:100%"></a> | <a href="https://static.igem.org/mediawiki/2021/b/b8/T--NCKU_Tainan--menbleonthespoon.jpg" target="_blank" style="width:50%"><img src="https://static.igem.org/mediawiki/2021/b/b8/T--NCKU_Tainan--menbleonthespoon.jpg" alt="" title="" style="width:100%"></a> | ||
− | <figcaption class="mt-3">Fig. 2. | + | <figcaption class="mt-3">Fig. 2. Menbles bubbles</figcaption> |
</figure> | </figure> | ||
</div> | </div> | ||
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<h3>f(int)</h3> | <h3>f(int)</h3> | ||
− | <p>  To assess how Menbles will behave within our intestine, without a doubt, performing a human clinical trial is required. However, due to the regulations of animal experiments and medical ethics that require a lengthy duration, it would be more desirable to build an in vitro platform of a human intestine that allows to bypass the restrictions and acquire the necessary information in much rapid and easy fashion. Inspired by the concept of “organ-on-a-chip”, we had built <b>f(int)</b>, a microfluidic chip that imitates the environment of human jejunum and enables us to determine the retention rate of bacteria inside the guts.</p> | + | <p>  To assess how Menbles will behave within our intestine, without a doubt, performing a human clinical trial is required. However, due to the regulations of animal experiments and medical ethics that require a lengthy duration, it would be more desirable to build an in vitro platform of a human intestine that allows to bypass the restrictions and acquire the necessary information in much rapid and easy fashion. Inspired by the concept of “organ-on-a-chip”, we had built<a href="https://2021.igem.org/Team:NCKU_Tainan/Hardware"><b>f(int)</b></a>, a microfluidic chip that imitates the environment of human jejunum and enables us to determine the retention rate of bacteria inside the guts.</p> |
<div class="container-fluid p-0"> | <div class="container-fluid p-0"> | ||
<div class="row no-gutters"> | <div class="row no-gutters"> |
Revision as of 15:58, 19 October 2021