Difference between revisions of "Team:NCKU Tainan/Parts"

(Prototype team page)
 
Line 1: Line 1:
−
{{IGEM_TopBar}}
+
{{Template:NCKU_Tainan/block_css}}
−
{{NCKU_Tainan}}
+
{{Template:NCKU_Tainan/bootstrap_min_css}}
−
<html>
+
{{Template:NCKU_Tainan/template_css}}
−
 
+
{{Template:NCKU_Tainan/header}}
−
 
+
{{Template:NCKU_Tainan/Parts_css}}
−
<div class="column full_size">
+
<html lang="en">
−
<h1>Parts</h1>
+
<head>
−
<p>Each team will make new parts during iGEM and will add them to the Registry of Standard Biological Parts. iGEM provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
+
  <meta name="viewport" content="width=device-width, initial-scale=1.0">
−
<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
+
  <meta charset="utf-8">
−
</div>
+
  <title>Collab</title>
−
 
+
</head>
−
<div class="column full_size">
+
<body data-spy="scroll" data-target=".sidenav">
−
<div class="highlight decoration_background">
+
<!--parts main body-->
−
<h3>Note</h3>
+
  <div class="content container-fluid">
−
<p>Note that parts must be well documented on each Part's Main Page on the <a href="http://parts.igem.org/Main_Page">Registry</a>. This documentation includes all of the characterization data for your parts. <b>The part's data MUST be on the part's Main Page on the Registry for your team to be eligible for medals and special prizes pertaining to parts.</b> <br><br>
+
    <div class="row justify-content-center">
−
This page serves to <i>showcase</i> the parts you have made and should include links to the Registry pages for your parts. Future teams and other users are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
+
      <!--Sidebar (Remember to change the subtitle context, but don't change the id)-->
−
</div>
+
      <aside class="sidenav d-none d-lg-flex flex-column justify-content-start align-items-center col-2">  
−
</div>
+
        <ul class="nav flex-column">
−
 
+
          <li class="nav-item">
−
<div class="clear extra_space"></div>
+
            <a class="nav-link" href="#Subtitle1">Basic Parts</a>
−
<div class="line_divider"></div>
+
          </li>
−
<div class="clear extra_space"></div>
+
          <li class="nav-item">
−
 
+
            <a class="nav-link" href="#Subtitle2">Composite Parts</a>
−
 
+
          </li>
−
 
+
        </ul>
−
 
+
      </aside>
−
 
+
      <!--two tables in here-->
−
<div class="column full_size">
+
      <main class="block-property margin-top-5">
−
<div class="highlight decoration_B_full">
+
      <!--The Page Name-->
−
 
+
<br></br>
−
<h3>Adding parts to the Registry</h3>
+
<img src="https://static.igem.org/mediawiki/2021/a/ac/T--NCKU_Tainan--parts.png" width="87%">
−
<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
+
−
 
+
−
<p>We encourage teams to start adding and documenting their parts on the Registry as soon as they can. Once you add your parts to the Registry, you can continue to add documentation to them throughout the iGEM season (up until the Registry freeze). This will allow you to remember all the details about your parts and store their history in the wiki. Documentation includes the characterization data of your parts.</p>
+
−
<div class="button_link">
+
−
<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
+
−
ADD PARTS
+
−
</a>
+
−
</div>
+
−
 
+
−
</div>
+
−
</div>
+
−
 
+
−
 
+
−
 
+
−
<div class="column third_size">
+
−
<div class="highlight decoration_A_full">
+
−
<h3>Inspiration: Basic Parts</h3>
+
−
<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
+
−
 
+
−
<p> You can also take a look at the following examples for Basic Parts on the Registry:</p>
+
−
<ul>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K3114006">2019 Calgary </a></li>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K3027000">2019 GO Paris Saclay</a></li>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K3187028">2019 TU Darmstadt</a></li>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K3552000">2020 Links China</a></li>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K3558000">2020 UNSW Australia</a></li>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K3338002">2020 Hannover</a></li>
+
−
</ul>
+
−
</div>
+
−
</div>
+
−
 
+
−
<div class="column third_size">
+
−
<div class="highlight decoration_A_full">
+
−
<h3>Inspiration: Composite Parts</h3>
+
−
<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
+
−
 
+
−
<p> You can also take a look at the following examples for Composite Parts on the Registry:</p>
+
−
<ul>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K3198007">2019 NUS Singapore </a></li>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K2932003">2019 Mingdao</a></li>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K2980009">2019 Tsinghua</a></li>
+
−
 
+
−
<li><a href="http://parts.igem.org/Part:BBa_K3407022">2020 TUDelft</a></li>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K3380500">2020 Edinburgh</a></li>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K3512042">2020 BITSPilani Goa India</a></li>
+
−
</ul>
+
−
</div>
+
−
</div>
+
−
 
+
−
 
+
−
 
+
−
<div class="column third_size">
+
−
 
+
−
<h3>What information do I need to start putting my parts on the Registry?</h3>
+
−
<p>The information needed to initially create a part on the Registry is:</p>
+
−
<ul>
+
−
<li>Part Name</li>
+
−
<li>Part type</li>
+
−
<li>Creator</li>
+
−
<li>Sequence</li>
+
−
<li>Short Description (60 characters on what the DNA does)</li>
+
−
<li>Long Description (Longer description of what the DNA does)</li>
+
−
<li>Design considerations</li>
+
−
</ul>
+
−
 
+
−
<p>
+
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, you must also put it up on the part page. </p>
+
−
 
+
−
</div>
+
−
 
+
−
 
+
−
<div class="clear extra_space"></div>
+
−
<div class="line_divider"></div>
+
−
<div class="clear extra_space"></div>
+
−
 
+
−
<div class="column full_size">
+
−
<h3>Part Table </h3>
+
−
 
+
−
<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
+
−
 
+
−
</html>
+
−
<groupparts>iGEM20 NCKU_Tainan</groupparts>
+
−
<html>
+
−
</div>
+
−
 
+
−
 
+
−
 
+
  
 +
        <!--overview part-->
 +
        <div style="margin-top:7%;">
 +
          <h2>Overview</h2>
 +
          <p style="padding-right: 10%;text-align: justify;">    BioBricks have always been the foundational building blocks for iGEM projects. When constructing our BioBricks, we made use of the IDT synthesis offer to generate our required parts in a fast and efficient way. Additionally, we made use of PCR amplification with our constructed expression plasmids as a template to construct several BioBricks. All of the parts contain a coding sequence, some of which extended with promoters or introns. We created a part collection comprised of 14 basic parts and 10 improved composite parts. All parts were generated during our project.</p>
 +
        </div>
 +
        <!--parts-->
 +
        <section>
 +
          <h2 id ="Subtitle1">Basic parts</h2>
 +
          <!--If you want to divide it into two block use below part-->
 +
          <div class="table-responsive" style="overflow-x:auto;">
 +
            <table class="table table-bordered table-hover">
 +
              <thead>
 +
                <tr>
 +
                  <th scope="col" style="text-align: center;">Name</th>
 +
                  <th scope="col" style="text-align: center;">Description</th>
 +
                  <th scope="col" style="text-align: center;">Type</th>
 +
                  <th scope="col" style="text-align: center;">Length</th>
 +
                </tr>
 +
              </thead>
 +
              <tbody>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_J23100">BBa_J23100</a></th>
 +
                  <td>Constitutive promoter (<i>P<sub>J23100</sub></i>)</td>
 +
                  <td style="text-align: center; vertical-align:middle;">Promotor</td>
 +
                  <td style="text-align: center; vertical-align:middle;">35bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K1123000">BBa_K1123000</a></th>
 +
                  <td>FNR promoter (<i>P<sub>fnr</sub></i>)</td>
 +
                  <td style="text-align: center; vertical-align:middle;">Promoter</td>
 +
                  <td style="text-align: center; vertical-align:middle;">120bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742146">BBa_ I742146</a></th>
 +
                  <td>Tyrosine ammonia-lyase native RBS (<i>N<sub>RBS</sub></i>)</td>
 +
                  <td style="text-align: center; vertical-align:middle;">RBS</td>
 +
                  <td style="text-align: center; vertical-align:middle;">14bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_B0017">BBa_B0017</a></th>
 +
                  <td>Double terminator (B0010-B0010)</td>
 +
                  <td style="text-align: center; vertical-align:middle;">Terminator</td>
 +
                  <td style="text-align: center; vertical-align:middle;">160bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997000">BBa_K2997000</a></th>
 +
                  <td>Tyrosine transporter (<i>tyrP</i>)</td>
 +
                  <td style="text-align: center; vertical-align:middle;">Coding</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1297bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742142">BBa_I742142</a></th>
 +
                  <td>Tyrosine ammonia-lyase (<i>sam8</i>)</td>
 +
                  <td style="text-align: center; vertical-align:middle;">Coding</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1536bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997001">BBa_K2997001</a></th>
 +
                  <td>Bacteriocin Full length (CBM-B)</td>
 +
                  <td style="text-align: center; vertical-align:middle;">Coding</td>
 +
                  <td style="text-align: center; vertical-align:middle;">867bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997002">BBa_K2997002</a></th>
 +
                  <td>Bacteriocin short length (sCBM-B)</td>
 +
                  <td style="text-align: center; vertical-align:middle;">Coding</td>
 +
                  <td style="text-align: center; vertical-align:middle;">252bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997003">BBa_K2997003</a></th>
 +
                  <td>Sigma 54-dependent transcriptional regulator (<i>pchR</i>)</td>
 +
                  <td style="text-align: center; vertical-align:middle;">Coding</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1854bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997004">BBa_K2997004</a></th>
 +
                  <td><i>pchR</i> binding range</td>
 +
                  <td style="text-align: center; vertical-align:middle;">Coding</td>
 +
                  <td style="text-align: center; vertical-align:middle;">567bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_J18921">BBa_J18921</a></th>
 +
                  <td>6aa [GS]x linker</td>
 +
                  <td style="text-align: center; vertical-align:middle;">Coding</td>
 +
                  <td style="text-align: center; vertical-align:middle;">18bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K1610300">BBa_K1610300</a></th>
 +
                  <td>Secretion tag (<i>yebF</i>)</td>
 +
                  <td style="text-align: center; vertical-align:middle;">Coding</td>
 +
                  <td style="text-align: center; vertical-align:middle;">354bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_E0040">BBa_E0040</a></th>
 +
                  <td>Wild type green fluorescence protein (<i>wtgfp</i>)</td>
 +
                  <td style="text-align: center; vertical-align:middle;">Coding</td>
 +
                  <td style="text-align: center; vertical-align:middle;">720bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2762017">BBa_K2762017</a></th>
 +
                  <td>Superfolder green fluorescence protein (<i>sfgfp</i>)</td>
 +
                  <td style="text-align: center; vertical-align:middle;">Coding</td>
 +
                  <td style="text-align: center; vertical-align:middle;">720bp</td>
 +
                </tr>
 +
              </tbody>
 +
            </table>
 +
          </div>
 +
        </section>
 +
        <!--comsposite parts-->
 +
        <section style="margin-bottom:5%;">
 +
        <h2 id ="Subtitle2">Composite Parts</h2>
 +
          <div class="table-responsive" style="overflow-x:auto;">
 +
            <table class="table table-bordered">
 +
              <thead>
 +
                <tr>
 +
                  <th scope="col" style="text-align: center;">Name</th>
 +
                  <th scope="col" style="text-align: center;">Description</th>
 +
                  <th scope="col" style="text-align: center;">Type</th>
 +
                  <th scope="col" style="text-align: center;">Length</th>
 +
                </tr>
 +
              </thead>
 +
              <tbody>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997005">BBa_K2997005</a></th>
 +
                  <td>K880005 - <i>yebF</i> - GS linker - sCBM-B</td>
 +
                  <td style="vertical-align:middle;text-align:center;" rowspan="9">Composite</td>
 +
                  <td style="text-align: center; vertical-align:middle;">685bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997006">BBa_K2997006</a></th>
 +
                  <td>K880005 - <i>yebF</i>- TB linker - sCBM-B</td>
 +
                  <td style="text-align: center; vertical-align:middle;">120bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997007">BBa_K2997007</a></th>
 +
                  <td>K880005 - <i>yebF</i> - CBM-B</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1282bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997008">BBa_K2997008</a></th>
 +
                  <td>Native promoter - <i>pchR</i> - sfgfp</td>
 +
                  <td style="text-align: center; vertical-align:middle;">3137bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997009">BBa_K2997009</a></th>
 +
                  <td>J23100 - <i>N<sub>RBS</sub></i> - <i>sam8</i></td>
 +
                  <td style="text-align: center; vertical-align:middle;">1606bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997010">BBa_K2997010</a></th>
 +
                  <td>K880005 - <i>sam8</i></td>
 +
                  <td style="text-align: center; vertical-align:middle;">1613bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997011">BBa_K2997011</a></th>
 +
                  <td>B0034 - <i>sam8</i></td>
 +
                  <td style="text-align: center; vertical-align:middle;">1572bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997012">BBa_K2997012</a></th>
 +
                  <td><i>P<sub>fnr</sub></i>-B0034-GFP</td>
 +
                  <td>866bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_K880005">BBa_K880005</a></th>
 +
                  <td>Strong promoter J23100 and strong RBS B0034</td>
 +
                  <td>Promoter+RBS</td>
 +
                  <td>55bp</td>
 +
                </tr>
 +
              </tbody>
 +
            </table>
 +
          </div>
 +
        </section>
 +
      </main>
 +
    </div>
 +
  </div>
 +
<script src="https://2019.igem.org/Team:NCKU_Tainan/js/jquery-3_4_1_min_js?action=raw&amp;ctype=text/javascript"></script>
 +
    <script src="https://2019.igem.org/Team:NCKU_Tainan/js/4.3.1/bootstrap_min_js?action=raw&amp;ctype=text/javascript"></script>
 +
<script>
 +
$(document).ready(function() {
 +
    //scroll spy
 +
    $("a[href^='#']").click(function(e) {
 +
        e.preventDefault();
 +
       
 +
        var position = $($(this).attr("href")).offset().top - 100;
 +
        console.log(position);
 +
        $("body, html").animate({
 +
            scrollTop: position
 +
        }, 200);
 +
    });
 +
});
 +
  $( document ).ready(function() {
 +
    $("#breadtext").html('<a href="https://2019.igem.org/Team:NCKU_Tainan">Home</a> > <a>Parts</a>')
 +
  });
 +
</script>
 +
</body>
 
</html>
 
</html>
 +
{{Template:NCKU_Tainan/gotop}}
 +
{{Template:NCKU_Tainan/footer}}

Revision as of 07:43, 11 August 2021

Collab


Overview

BioBricks have always been the foundational building blocks for iGEM projects. When constructing our BioBricks, we made use of the IDT synthesis offer to generate our required parts in a fast and efficient way. Additionally, we made use of PCR amplification with our constructed expression plasmids as a template to construct several BioBricks. All of the parts contain a coding sequence, some of which extended with promoters or introns. We created a part collection comprised of 14 basic parts and 10 improved composite parts. All parts were generated during our project.

Basic parts

Name Description Type Length
BBa_J23100 Constitutive promoter (PJ23100) Promotor 35bp
BBa_K1123000 FNR promoter (Pfnr) Promoter 120bp
BBa_ I742146 Tyrosine ammonia-lyase native RBS (NRBS) RBS 14bp
BBa_B0017 Double terminator (B0010-B0010) Terminator 160bp
BBa_K2997000 Tyrosine transporter (tyrP) Coding 1297bp
BBa_I742142 Tyrosine ammonia-lyase (sam8) Coding 1536bp
BBa_K2997001 Bacteriocin Full length (CBM-B) Coding 867bp
BBa_K2997002 Bacteriocin short length (sCBM-B) Coding 252bp
BBa_K2997003 Sigma 54-dependent transcriptional regulator (pchR) Coding 1854bp
BBa_K2997004 pchR binding range Coding 567bp
BBa_J18921 6aa [GS]x linker Coding 18bp
BBa_K1610300 Secretion tag (yebF) Coding 354bp
BBa_E0040 Wild type green fluorescence protein (wtgfp) Coding 720bp
BBa_K2762017 Superfolder green fluorescence protein (sfgfp) Coding 720bp

Composite Parts

Name Description Type Length
BBa_K2997005 K880005 - yebF - GS linker - sCBM-B Composite 685bp
BBa_K2997006 K880005 - yebF- TB linker - sCBM-B 120bp
BBa_K2997007 K880005 - yebF - CBM-B 1282bp
BBa_K2997008 Native promoter - pchR - sfgfp 3137bp
BBa_K2997009 J23100 - NRBS - sam8 1606bp
BBa_K2997010 K880005 - sam8 1613bp
BBa_K2997011 B0034 - sam8 1572bp
BBa_K2997012 Pfnr-B0034-GFP 866bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_K880005 Strong promoter J23100 and strong RBS B0034 Promoter+RBS 55bp

e-mail : igem.ncku.tainan@gmail.com
phone : 06-2757575