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<div style="margin-top:7%;"> | <div style="margin-top:7%;"> | ||
<h2>Overview</h2> | <h2>Overview</h2> | ||
− | <p style="padding-right: 10%;text-align: justify;"> | + | <p style="padding-right: 10%;text-align: justify;">  BioBricks have always been the foundational building blocks for iGEM projects. When constructing our BioBricks, we made use of the IDT synthesis offer to generate our required parts in a fast and efficient way. Additionally, we made use of PCR amplification with our constructed expression plasmids as a template to construct several BioBricks. All of the parts contain a coding sequence, some of which extended with promoters or introns. We created a part collection comprised of 29 basic parts and 48 improved composite parts. All parts were generated during our project.</p> |
</div> | </div> | ||
<!--parts--> | <!--parts--> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part: | + | <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K3771100">BBa_K3771100</a></th> |
<td>SoxR</td> | <td>SoxR</td> | ||
<td style="text-align: center; vertical-align:middle;">Coding</td> | <td style="text-align: center; vertical-align:middle;">Coding</td> | ||
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<tr> | <tr> | ||
<th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K3771026">BBa_K3771026</a></th> | <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K3771026">BBa_K3771026</a></th> | ||
− | <td><i>P<sub>pspA</sub></i>-<i> | + | <td><i>P<sub>pspA</sub></i>-<i>jju</i>-<i>P<sub>lacI</sub></i>-<i>ompA</i>/<i>oprF</i></td> |
<td style="text-align: center; vertical-align:middle;">2661bp</td> | <td style="text-align: center; vertical-align:middle;">2661bp</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K3771027">BBa_K3771027</a></th> | <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K3771027">BBa_K3771027</a></th> | ||
− | <td><i>P<sub>pspA</sub></i>-<i> | + | <td><i>P<sub>pspA</sub></i>-<i>jju</i>-<i>6xHis</i>-<i>P<sub>lacI</sub></i>-<i>ompA</i>/<i>oprF</i></td> |
<td style="text-align: center; vertical-align:middle;">2679bp</td> | <td style="text-align: center; vertical-align:middle;">2679bp</td> | ||
</tr> | </tr> | ||
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<tr> | <tr> | ||
<th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K3771032">BBa_K3771032</a></th> | <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K3771032">BBa_K3771032</a></th> | ||
− | <td><i>P<sub>pspA</sub></i>-<i> | + | <td><i>P<sub>pspA</sub></i>-<i>jju</i>-<i>P<sub>ompA</sub></i>-<i>ompA</i>/<i>oprF</i></td> |
<td style="text-align: center; vertical-align:middle;">2764bp</td> | <td style="text-align: center; vertical-align:middle;">2764bp</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K3771033">BBa_K3771033</a></th> | <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K3771033">BBa_K3771033</a></th> | ||
− | <td><i>P<sub>pspA</sub></i>-<i> | + | <td><i>P<sub>pspA</sub></i>-<i>jju</i>-<i>6xHis</i>-<i>P<sub>ompA</sub></i>-<i>ompA</i>/<i>oprF</i></td> |
<td style="text-align: center; vertical-align:middle;">2782bp</td> | <td style="text-align: center; vertical-align:middle;">2782bp</td> | ||
</tr> | </tr> | ||
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<tr> | <tr> | ||
<th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K3771079">BBa_K3771079</a></th> | <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K3771079">BBa_K3771079</a></th> | ||
− | <td><i>P<sub>T7</sub></i>-<i> | + | <td><i>P<sub>T7</sub></i>-<i>jju</i></td> |
<td style="text-align: center; vertical-align:middle;">1222bp</td> | <td style="text-align: center; vertical-align:middle;">1222bp</td> | ||
</tr> | </tr> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part: | + | <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K3771100">BBa_K3771100</a></th> |
<td><i>soxR</i></td> | <td><i>soxR</i></td> | ||
<td style="text-align: center; vertical-align:middle;">Coding</td> | <td style="text-align: center; vertical-align:middle;">Coding</td> |
Latest revision as of 12:32, 21 October 2021
![](https://static.igem.org/mediawiki/2021/4/44/T--NCKU_Tainan--paet_header.png)
Overview
BioBricks have always been the foundational building blocks for iGEM projects. When constructing our BioBricks, we made use of the IDT synthesis offer to generate our required parts in a fast and efficient way. Additionally, we made use of PCR amplification with our constructed expression plasmids as a template to construct several BioBricks. All of the parts contain a coding sequence, some of which extended with promoters or introns. We created a part collection comprised of 29 basic parts and 48 improved composite parts. All parts were generated during our project.
Basic parts
Name | Description | Type | Length |
---|---|---|---|
BBa_R0010 | lacI promoter | Promoter | 200bp |
BBa_K156009 | OFP | Coding | 669bp |
BBa_K3071013 | pspA promoter | Promoter | 218bp |
BBa_K3771009 | OmpA/OprF Chimeric Protein | Coding | 1110bp |
BBa_K3771011 | ompA promoter | Promoter | 303bp |
BBa_K3771010 | OmpA | Coding | 1041bp |
BBa_K3771008 | CSAD-6xHis | Coding | 1500bp |
BBa_J23100 | J23100 | Promoter | 35bp |
BBa_J23101 | J23101 | Promoter | 35bp |
BBa_K3771047 | PkatG | Promoter | 45bp |
BBa_K3771048 | PsoxS | Promoter | 85bp |
BBa_K2862010 | PsoxS mutant3 (Imperial) | Promoter | 61bp |
BBa_B0034 | B0034 | RBS | 12bp |
BBa_K2918012 | RiboJ | Regulator | 75bp |
BBa_K3771100 | SoxR | Coding | 465bp |
BBa_K1321337 | sfGFP | Coding | 711bp |
BBa_K592012 | RCP | Coding | 681bp |
BBa_K3771012 | CS-6xHis | Coding | 1275bp |
BBa_K3771100 | SoxR (reverse) | Coding | 1275bp |
BBa_K3771000 | JJU | Coding | 465bp |
BBa_K3771006 | JJU-6xHis | Coding | 1191bp |
BBa_K3771001 | CoaBC | Coding | 1221bp |
BBa_K3771007 | CoaBC-6xHis | Coding | 1239bp |
BBa_K3771004 | CDO1 | Coding | 603bp |
BBa_K3771003 | CSAD | Coding | 1482bp |
BBa_K3771002 | CS | Coding | 1257bp |
BBa_K3771041 | CDO1-6xHis | Coding | 1275bp |
BBa_K864400 | Ptrc | Promoter | 30bp |
BBa_I719005 | PT7 | Promoter | 23bp |
Composite Parts
Name | Description | Type | Length |
---|---|---|---|
BBa_K3771013 | PlacI-ofp | Composite | 1090bp |
BBa_K3771014 | PpspA-ofp | 1008bp | |
BBa_K3771015 | PlacI-ompA/oprF | 1336bp | |
BBa_K3771016 | PompA-ompA/oprF | 1439bp | |
BBa_K3771017 | PpspA-ofp-PlacI-ompA/oprF | 2352bp | |
BBa_K3771018 | PpspA-ofp-PompA-ompA/oprF | 2455bp | |
BBa_K3771020 | PpspA-jju | 1317bp | |
BBa_K3771021 | PpspA-jju-6xHis | 1335bp | |
BBa_K3771022 | PpspA-coaBC | 1365bp | |
BBa_K3771023 | PpspA-coaBC-6xHis | 1383bp | |
BBa_K3771024 | PpspA-csad | 1626bp | |
BBa_K3771025 | PpspA-csad-6xHis | 1644bp | |
BBa_K3771026 | PpspA-jju-PlacI-ompA/oprF | 2661bp | |
BBa_K3771027 | PpspA-jju-6xHis-PlacI-ompA/oprF | 2679bp | |
BBa_K3771028 | PpspA-coaBC-PlacI-ompA/oprF | 2709bp | |
BBa_K3771029 | PpspA-coaBC-6xHis-PlacI-ompA/oprF | 2727bp | |
BBa_K3771030 | PpspA-csad-PlacI-ompA/oprF | 2970bp | |
BBa_K3771031 | PpspA-csad-6xHis-PlacI-ompA/oprF | 2988bp | |
BBa_K3771032 | PpspA-jju-PompA-ompA/oprF | 2764bp | |
BBa_K3771033 | PpspA-jju-6xHis-PompA-ompA/oprF | 2782bp | |
BBa_K3771034 | PpspA-coaBC-PompA-ompA/oprF | 2812bp | |
BBa_K3771035 | PpspA-coaBC-6xHis-PompA-ompA/oprF | 2830bp | |
BBa_K3771036 | PpspA-csad-PompA-ompA/oprF | 3073bp | |
BBa_K3771037 | PpspA-csad-6xHis-PompA-ompA/oprF | 3091bp | |
BBa_K3771038 | PpspA-cs | 1401bp | |
BBa_K3771039 | PpspA-cs-6xHis | 1419bp | |
BBa_K3771040 | PpspA-cdo1 | 747bp | |
BBa_K3771042 | PpspA-cdo1-6xHis | 765bp | |
BBa_K3771043 | PpspA-cs-PompA-ompA/oprF | 2848bp | |
BBa_K3771044 | PpspA-cs-6xHis-PompA-ompA/oprF | 2212bp | |
BBa_K3771045 | PpspA-cdo1-PompA-ompA/oprF | 2194bp | |
BBa_K3771046 | PpspA-cdo1-6xHis-PompA-ompA/oprF | 2212bp | |
BBa_K3771050 | J23100-sfgfp | 774bp | |
BBa_K3771091 | PkatG-sfgfp | 784bp | |
BBa_K3771092 | PsoxS-sfgfp | 824bp | |
BBa_K3771093 | soxR-PsoxS-sfgfp | 1297bp | |
BBa_K3771094 | PsoxS-cs-6xHis | 1386bp | |
BBa_K3771095 | soxR-PsoxS-cs-6xHis | 1859bp | |
BBa_K3771096 | soxR-PsoxS-cdo1-6xHis | 1205bp | |
BBa_K3771097 | soxR-PsoxS-csad-6xHis | 2084bp | |
BBa_K3771098 | PsoxS-RiboJ-rcp-J23101-soxR | 1409bp | |
BBa_K3771099 | PsoxS-RiboJ-sfgfp-J23101-soxR | 1441bp | |
BBa_K3771075 | Ptrc-cdo1 | 690bp | |
BBa_K3771076 | PlacI-csad | 1708bp | |
BBa_K3771077 | Ptrc-csad | 1569bp | |
BBa_K3771078 | Ptrc-cs | 1344bp | |
BBa_K3771079 | PT7-jju | 1222bp | |
BBa_K3771080 | PT7-coaBC | 1270bp |
Parts Collection
Function | Name | Description | Type | Length |
---|---|---|---|---|
Detection of Depressive Symptoms | BBa_K3771048 | PsoxS | Promoter | 85bp |
BBa_K3771100 | soxR | Coding | 465bp | |
BBa_K3771092 | PsoxS-sfgfp | Composite | 824bp | |
BBa_K3771093 | soxR-PsoxS-sfgfp | Composite | 1297bp | |
BBa_K3071013 | pspA promoter | Promoter | 118bp | |
BBa_K156009 | OFP | Coding | 864bp | |
BBa_K3771009 | OmpA/OprF Chimeric Protein | Coding | 1110bp | |
BBa_K3771017 | PpspA-ofp-PlacI-ompA/oprF | Composite | 2352bp | |
BBa_K3771018 | PpspA-ofp-PompA-ompA/oprF | Composite | 2455bp | |
Taurine production | BBa_K3771097 | soxR-PsoxS-csad-6xHis | Composite | 2084bp |
BBa_K3771044 | PpspA-cs-6xHis-PompA-ompA/oprF | Composite | 2212bp | |
BBa_K3771075 | Ptrc-cdo1 | Composite | 690bp | |
BBa_K3771000 | JJU | Coding | 1173bp | |
BBa_K3771001 | CoaBC | Coding | 1221bp |
![](https://static.igem.org/mediawiki/2021/1/18/T--NCKU_Tainan--backtotop.png)