Difference between revisions of "Team:NCKU Tainan/Parts"

Line 240: Line 240:
 
               <tbody>
 
               <tbody>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997005">BBa_K2997005</a></th>
+
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K3771013">BBa_K3771013</a></th>
−
                   <td>K880005 - <i>yebF</i> - GS linker - sCBM-B</td>
+
                   <td><i>P<sub>pLacI</sub></i>-OFP</td>
 
                   <td style="vertical-align:middle;text-align:center;" rowspan="9">Composite</td>
 
                   <td style="vertical-align:middle;text-align:center;" rowspan="9">Composite</td>
−
                   <td style="text-align: center; vertical-align:middle;">685bp</td>
+
                   <td style="text-align: center; vertical-align:middle;">1070bp</td>
 
                 </tr>
 
                 </tr>
 
                 <tr>
 
                 <tr>
Line 285: Line 285:
 
                   <td style="text-align: center; vertical-align:middle;">1550bp</td>
 
                   <td style="text-align: center; vertical-align:middle;">1550bp</td>
 
                 </tr>
 
                 </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                  <td><i>sam8</i> with ribosome binding site</td>
 +
                  <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
               
 +
               
 +
               
 +
               
 +
               
 +
               
 +
               
 +
               
 
                 <tr>
 
                 <tr>
 
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_K880005">BBa_K880005</a></th>
 
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_K880005">BBa_K880005</a></th>

Revision as of 05:30, 19 October 2021

Overview

BioBricks have always been the foundational building blocks for iGEM projects. When constructing our BioBricks, we made use of the IDT synthesis offer to generate our required parts in a fast and efficient way. Additionally, we made use of PCR amplification with our constructed expression plasmids as a template to construct several BioBricks. All of the parts contain a coding sequence, some of which extended with promoters or introns. We created a part collection comprised of 14 basic parts and 10 improved composite parts. All parts were generated during our project.

Basic parts

Name Description Type Length
BBa_R0010 lacI promoter Promoter 200bp
BBa_K156009 OFP Coding 669bp
BBa_K3071013 pspA promoter Promoter 218bp
BBa_K3771009 OmpA/OprF Chimeric Protein Coding 1110bp
BBa_K3771011 ompA promoter Promoter 303bp
BBa_K3771010 OmpA Coding 1041bp
BBa_K3771008 CSAD-6xHis Coding 1500bp
BBa_J23100 J23100 Promoter 35bp
BBa_J23101 J23101 Promoter 35bp
BBa_K3771047 PkatG Promoter 45bp
BBa_K3771048 PsoxS Promoter 85bp
BBa_K2862010 PsoxS (Imperial, mutant3) Promoter 61bp
BBa_B0034 B0034 RBS 12bp
BBa_K2918012 RiboJ Regulator 75bp
BBa_K223000 soxR Coding 465bp
BBa_K1321337 sfGFP Coding 711bp
BBa_K592012 RCP Coding 681bp
BBa_K3771012 CS-6xHis Coding 1275bp
BBa_K3771000 JJU Coding 1173bp
BBa_K3771006 JJU-6xHis Coding 1191bp
BBa_K3771001 CoaBC Coding 1221bp
BBa_K3771007 CoaBC-6xHis Coding 1239bp
BBa_K3771004 CDO1 Coding 603bp
BBa_K3771003 CSAD Coding 1482bp
BBa_K3771002 CS Coding 1257bp
BBa_K3771041 CDO1-6xHis Coding 1275bp
BBa_K864400 Ptrc Promoter 30bp
BBa_I719005 T7 Promoter 23bp

Composite Parts

Name Description Type Length
BBa_K3771013 PpLacI-OFP Composite 1070bp
BBa_K2997006 K880005 - yebF- TB linker - sCBM-B 120bp
BBa_K2997007 K880005 - yebF - CBM-B 1282bp
BBa_K2997008 Native promoter - pchR - sfgfp 3137bp
BBa_K2997009 J23100 - NRBS - sam8 1606bp
BBa_K2997010 K880005 - sam8 1613bp
BBa_K2997011 B0034 - sam8 1572bp
BBa_K2997012 Pfnr-B0034-GFP 866bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_K880005 Strong promoter J23100 and strong RBS B0034 Promoter+RBS 55bp

e-mail : igem.ncku.tainan@gmail.com
phone : 06-2757575