Team:SCUT-China/Notebook
2020.7——recruit our first team members
2020.7-8——select our theme ; Learn about iGEM competitions in the past ; Learn experimental operations and basic knowledge.
2020.9-2021.2——Discuss with PI ; Learn experimental operations ; Conduct literature research to find a suitable topic.
2021.2.10——Determine the theme.
2021.2.20——Conduct literature research ; search for promotor.
2021.2.20——Software: Understand the HMM ; Determine NuPoP as our evaluation function.
2021.3.4——Fermenter: preliminarily determined The growth of simulated bacteria in fermentation tank.
2021.3.9——team officially formed.
2021.3.17——Software: Attempts at machine learning thwarted ; Discover NucOpt Progs ; Identifying problems and proposing better solutions.
2021.3.28——Officially registered our iGEM team.
2021.4.4——Held 2021 iGEM Guangzhou-Shenzhen District Symposium.
2021.4.5——Acquisition the fragments of promoter and terminator.
2021.4.7——Fermenter: Writing an ODE set hits a snag, shifts direction to science and visualisation.
2021.4.10——Construct the vector of YEp181-Promoter-SAG1t.
2021.4.17——Acquisition fragment of the VS gene (1770 bp).
2021.4.20——Construct the vector of YEp181-Promoter-VS-SAG1t .
2021.4.21——Fermenter: ODE set was completed and put into the HP education.
2021.4.22——The First volunteer teaching at Tuhua Primary School.
2021.4.29——preparation for competent cell of Saccharomyces cerevisiae BJ5464.
2021.5.1——Transformation of Cas9 plasmids into brewer's yeast.
2021.5.5——Software: Complete the NuPGO code ; Successful implementation of promoter optimisation.
2021.5.6——The second volunteer teaching at Tuhua Primary School.
2021.5.8——Knock-in of the Promoter-VS-SAG1t expression cassettes into the genome of S. cerevisiae BJ5464.
2021.5.15——Fermentation and testing of valencene by S. cerevisiae.
2021.5.16——Held the Synthetic Biology X Symposium.
2021.6.2——Fermenter:Revised after consultation with the professor.
2021.6.9——Construction of M1 M2 promotor by overlap ; construct vector of YEp181-promoter-VS-SAG1t.
2021.6.14——Knock-in of the Promoter M1 M2 into the genome of S. cerevisiae.
2021.6.19——The first modification of brewer's yeast fermentation for the production of valencene and its detection.
2021.7.15——Construction of M3 M4 promotor by overlap ; construct vector of YEp181-promoter-VS-SAG1t.
2021.7.21—Knock-in of the Promoter M3-M7 into the genome of S. cerevisiae.
2021.7.24——The second modification of brewer's yeast fermentation for the production of valencene and its detection.
2021.7.28——Construction of M8-M410 promotor by overlap ; construct vector of YEp181-promoter-VS-SAG1t.
2021.8——Machine Learning: Literature research to obtain dataset and prepare training code.
2021.8.3——Knock-in of the Promoter M3-M7 into the genome of S. cerevisiae.
2021.8.7——The third modification of brewer's yeast fermentation for the production of valencene and its detection.
2021.8.8——Lecture of Synthetic Biology in Guangzhou NO.5 Middle School.
2021.8.22——Lecture of Synthetic Biology in Ganzhou Middle School.
2021.8.25——Lecture of Synthetic Biology in Foshan No.1 High School.
2021.8.25——Lecture of Synthetic Biology in Taian Yingxiongshan Middle School.
2021.8.29——Lecture of Synthetic Biology in Zhejiang Yanzhou Middle School.
2021.9——Machine Learning: Initial acquisition of training results ; Analysis, parameter optimisation.
2021.9.12——Fermentation of the constructed.
2021.9.12——Lecture of Synthetic Biology in Guangdong Beijiang Secondary School.
2021.9.20——Re-fermentation experiments with brewer's yeast containing M8p and PDC1p ; Perform qPCR experiments to verify increased levels of transcription.
2021.9.20——Lecture of Synthetic Biology in Guangdong Guangya Middle School
2021.9.20——Lecture of Synthetic Biology in LanLian No.1 Middle School.
2021.9.21——Complete M8 optimisation and more validation and comparison ; Validating the effects of NuPGO.
2021.9.25——The plasmid YEP352-PPADH3-ADH1T-M8P-VS-SAG1T was constructed by homologous recombination.
2021.9.25——Construction of Yep352-PpADH3-ADH1t-M8p-VS-SAG1t by homologous recombination.
2021.10——Machine Learning: Continued parameter optimisation attempts and comparison of results.
2021.10.3——Preparation for competent cell of Saccharomyces cerevisiae CEN.PK2-1Ca.
2021.10.5——Transform Yep352-PpADH3-ADH1t-M8p-VS-SAG1t plasmid containing HPO、CPR gene and YEp352-Hm6-HPO-TPI1t-CYC1p-AtCPR-CYC1t plasmid into Saccharomyces cerevisiae CEN.PK2-1Ca.
2021.10.5——Transform Yep352-PpADH3-ADH1t-PDC1p-VS-SAG1t plasmid containing HPO、CPR gene and YEp352-Hm6-HPO-TPI1t-CYC1p-AtCPR-CYC1t plasmid into Saccharomyces cerevisiae CEN.PK2-1Ca.
2021.10.7——Fermentation experiments of M8 (CEN.PK2-1Ca) and PDC1 (CEN.PK2-1Ca) ; Sample preparation and detection of the supernatant of the fermentation broth by gas chromatographic.