Team:NCHU Taichung/Note
Note
2020 Apr-Jun
Design PQQ plasmid
Transfer PQQ gene into Bacillus subtilis WB800N
B.subtilis WB800/PQQ growth curve measurement
Sow the seed of Arabidopsis thaliana
Inoculate Arabidopsis with Bacillus subtilis WB800N/PQQ and Bacillus subtilis WB800 .
Control group only inoculated water.
PQQ plasmid extraction
Record the phenotype of Arabidopsis
Bacillus subtilis WB800 with PQQ plasmid improved the growth of Arabidopsis.
Bacillus subtilis WB800 with PQQ plasmid improved the growth of Arabidopsis.
2020 Jul-Sep
Rice straw decomposition
Use four kinds of cellulose to decomposition rice straw, in order to get more sugar in the solution.
The result of the DNS reagent shows that, with cellulose, the sugar in solution is higher.
Use four kinds of cellulose to decomposition rice straw, in order to get more sugar in the solution.
The result of the DNS reagent shows that, with cellulose, the sugar in solution is higher.
Meetup Conference
Transfer PQQ plasmid into Bacillus subtilis RM125 and extract it.
2020 Oct-Dec
growth curve measurement
Sow the seed of Arabidopsis.
1. Inoculation.
Inoculate Arabidopsis with Bacillus subtilis RM125/PQQ and Bacillus subtilis RM125. Control group only inoculated water.
2. Sow the seed of Arabidopsis
Inoculate Arabidopsis with Bacillus subtilis RM125/PQQ and Bacillus subtilis RM125. Control group only inoculated water.
2. Sow the seed of Arabidopsis
Record the phenotype of Arabidopsis.
The result shows that Bacillus subtilis RM125 with PQQ plasmid improved the growth of Arabidopsis. But the sample size was small this time. We need to repeat this experiment again and increase the sample size.
The result shows that Bacillus subtilis RM125 with PQQ plasmid improved the growth of Arabidopsis. But the sample size was small this time. We need to repeat this experiment again and increase the sample size.
Bacillus subtilis RM125 16s rDNA PCR
Inoculation
Inoculate Arabidopsis with the supernatant of Bacillus subtilis RM125/PQQ and Bacillus subtilis RM125. Control group only inoculated water.
Inoculate Arabidopsis with the supernatant of Bacillus subtilis RM125/PQQ and Bacillus subtilis RM125. Control group only inoculated water.
Inoculation
Inoculate the supernatant of Bacillus subtilis RM125/PQQ and Bacillus subtilis RM125 again.
Inoculate the supernatant of Bacillus subtilis RM125/PQQ and Bacillus subtilis RM125 again.
Record the phenotype of Arabidopsis.
Supernatant of Bacillus improved the growth of Arabidopsis
Supernatant of Bacillus improved the growth of Arabidopsis
2021 Jan-Mar
Find the best carbon-nitrogen ratio of the saccharified straw liquid
We think the straw solution lacks nitrogen, bacteria can’t grow well, so we add okara solution to find the carbon nitrogen ratio Bacillus subtilis RM125/PQQ most like.
Mix okara solution and straw solution in different formulas, and use them to culture.
Then find the best formula is 80% okara solution plus 20% straw solution.
We think the straw solution lacks nitrogen, bacteria can’t grow well, so we add okara solution to find the carbon nitrogen ratio Bacillus subtilis RM125/PQQ most like.
Mix okara solution and straw solution in different formulas, and use them to culture.
Then find the best formula is 80% okara solution plus 20% straw solution.
Sow the dehull rice seed in MS medium.
Inoculation
Inoculated rice seeding with Bacillus subtilis RM125/PQQ and Bacillus subtilis RM125.
Inoculated rice seeding with Bacillus subtilis RM125/PQQ and Bacillus subtilis RM125.
Re-isolation
Record the phenotype of rice.
Collaboration
Discussed modeling with 2021 Mingdao iGEM team instructor Dr. Pei-Hong Chen.
Discussed modeling with 2021 Mingdao iGEM team instructor Dr. Pei-Hong Chen.
2021 Apr-Jun
Sow the seed of Arabidopsis.
Use saccharified straw liquid to culture Bacillus subtilis RM125/PQQ, and find the growth
curve.
Inoculation
Inoculate Arabidopsis with Bacillus subtilis RM125/PQQ and Bacillus subtilis RM125. Control group only inoculated water.
Inoculate Arabidopsis with Bacillus subtilis RM125/PQQ and Bacillus subtilis RM125. Control group only inoculated water.
Arabidopsis phenotype recording I
Sow rice seeds in pot soil
Arabidopsis phenotype recording II
Covid-19 outbreak
Many members left the school
Many members left the school
Rice inoculation I
Use saccharified straw liquid as culture medium and inoculate the supernatant of Bacillus subtilis RM125/PQQ.
The concentration of the supernatant is 1x, 1/100x,1/200x, and 1/500x. Control group inoculated water.
Use saccharified straw liquid as culture medium and inoculate the supernatant of Bacillus subtilis RM125/PQQ.
The concentration of the supernatant is 1x, 1/100x,1/200x, and 1/500x. Control group inoculated water.
Rice inoculation II
Phenotype recording I
2021 Jul-Sep
Collaboration with Info-Tech Services
1. Online meetup conference
2. Measure chlorophyll content and plant weight
Rice inoculation III
Phenotype recording III
Data analyzing and integration
Data discussion I
Interviews with Taiwan Riceplant Association, Mr. Liang Huoqiu, and several of its members
Data discussion II
Coffee with jamboree
PanSci Podcast
B. subtilis RM125/PQQ can be endophyte.
The picture below shows the PQQ plasmid extracted from re-isolated bacteria.
The picture below shows the PQQ plasmid extracted from re-isolated bacteria.
Re-isolated Bacillus subtilis RM125/PQQ PCR.
Double check the bacteria re-isolated was Bacillus subtilis RM125/PQQ
Double check the bacteria re-isolated was Bacillus subtilis RM125/PQQ
2021 Oct-Now
Interview with Nantou Environmental Protection Bureau
- nchutaichung2021@gmail.com
- NCHU iGEM Team
- igem_nchu_taichung
- NCHU_Taichung
- NCHU, Taichung, Taiwan
