Short description:pET28-A inserted with pqqA, pqqB, pqqC, pqqD, pqqE, f1 ori, T7 terminator
and pr promoter.
Long description:The purification of PQQ compound is very difficult.The concentration of PQQ
secrete from bacteria may too low to be detected by HPLC.However, We found that the OD value
in the log phase and the stationary phase of Bacillus subtilis RM125/PQQ raise.
We conducted plasmid extraction and PCR to verify that the PQQ plasmid was successfully
transferred into Bacillus subtilis RM125.
Name: xynC BBa_K3830016
Short description: pGET-118 inserted with xynC, T7 terminator and pr promoter
Long description: we use this plasmid to degrade hemicellulose at the non-reducing end and
we inserted pr promoter to enhance the express of this plasmid
Name: celK BBa_K3830017
Short description: pGET-118 inserted with celK, T7 terminator and pr promoter
Long description: we use this plasmid to hydrolyze the reducing or non-reducing end to xylan
disaccharide and we inserted pr promoter to enhance the express of this plasmid
Name: celA BBa_K3830018
Short description: pGET-118 inserted with celA, T7 terminator and pr promoter
Long description: we use this plasmid to hydrolyze the β-1,4 bond by producing a new
reduction end at the loosely arranged amorphous region then make the new reducing end at
long chain molecule and we inserted pr promoter to enhance the express of this plasmid
Name: cipA BBa_K3830019
Short description: pGET-118 inserted with cipA, T7 terminator and pr promoter
Long description: we use this plasmid to promotes binding of cellulose to the catalytic
domains of the cellulolytic enzymes and we inserted pr promoter to enhance the express of
this plasmid.
