Team:Evry Paris-Saclay/Composite Parts
Our
Composite Parts
Composite Parts
During this project, our team designed the following composite parts.
The expression cassettes of the Evolution.T7 mutators
| Part Number | Part Name |
|---|---|
| BBa_K3766100 | T7RNAP CGG-R12-KIRV expression cassette under the control of pBad promoter |
| BBa_K3766101 | AID T7RNAP CGG-R12-KIRV expression cassette under the control of pBad promoter |
| BBa_K3766102 | pmCDA1 T7RNAP CGG-R12-KIRV expression cassette under the control of pBad promoter |
| BBa_K3766103 | rAPOBEC1 T7RNAP CGG-R12-KIRV expression cassette under the control of pBad promoter |
| BBa_K3766104 | TadA* T7RNAP CGG-R12-KIRV expression cassette under the control of pBad promoter |
| BBa_K3766105 | evoAPOBEC1-BE4max T7RNAP CGG-R12-KIRV expression cassette under the control of pBad promoter |
| BBa_K3766106 | evoCDA1-BE4max T7RNAP CGG-R12-KIRV expression cassette under the control of pBad promoter |
| BBa_K3766107 | ABE8.20-m T7RNAP CGG-R12-KIRV expression cassette under the control of pBad promoter |
| BBa_K3766108 | evoAPOBEC1-BE4max T7RNAP expression cassette under the control of pTetA promoter |
| BBa_K3766109 | evoCDA1-BE4max T7RNAP expression cassette under the control of pTetA promoter |
| BBa_K3766110 | ABE8.20-m T7RNAP expression cassette under the control of pTetA promoter |
The expression cassettes of aztreonam resistance genes, the AmpR variants evolved to confer resistance to aztreonam
| Part Number | Part Name |
|---|---|
| BBa_K3766151 | AZT-1 expression cassette |
| BBa_K3766152 | AZT-2 expression cassette |
| BBa_K3766153 | AZT-3 expression cassette |
| BBa_K3766154 | AZT-4 expression cassette |
| BBa_K3766155 | AZT-5 expression cassette |
| BBa_K3766156 | AZT-6 expression cassette |
| BBa_K3766157 | AZT-7 expression cassette |
| BBa_K3766158 | AZT-8 expression cassette |
| BBa_K3766159 | AZT-9 expression cassette |
| BBa_K3766160 | AZT-10 expression cassette |
| BBa_K3766161 | AZT-11 expression cassette |
| BBa_K3766162 | AZT-12 expression cassette |
| BBa_K3766163 | AZT-13 expression cassette |
| BBa_K3766164 | AZT-14 expression cassette |
| BBa_K3766165 | AZT-15 expression cassette |
| BBa_K3766166 | AZT-16 expression cassette |
| BBa_K3766167 | AZT-17 expression cassette |
| BBa_K3766168 | AZT-18 expression cassette |
| BBa_K3766169 | AZT-19 expression cassette |
| BBa_K3766170 | AZT-20 expression cassette |
| BBa_K3766171 | AZT-21 expression cassette |
| BBa_K3766172 | AZT-22 expression cassette |
| BBa_K3766173 | AZT-23 expression cassette |
| BBa_K3766174 | AZT-24 expression cassette |
| BBa_K3766175 | AZT-25 expression cassette |
| BBa_K3766176 | AZT-26 expression cassette |
| BBa_K3766177 | AZT-27 expression cassette |
| BBa_K3766178 | AZT-28 expression cassette |
| BBa_K3766179 | AZT-29 expression cassette |
| BBa_K3766180 | AZT-30 expression cassette |
| BBa_K3766181 | AZT-31 expression cassette |
| BBa_K3766182 | AZT-32 expression cassette |
| BBa_K3766183 | AZT-33 expression cassette |
| BBa_K3766184 | AZT-34 expression cassette |
| BBa_K3766185 | AZT-35 expression cassette |
The expression cassettes of LacI variants
| Part Number | Part Name |
|---|---|
| BBa_K3766190 | wild type LacI expression cassette |
| BBa_K3766191 | LacI K325R expression cassette |
Our reporter constructs
| Part Number | Part Name |
|---|---|
| BBa_K3766111 | mCherry sfGFP AmpR in the Evolution.T7 mutational region |
| BBa_K3766112 | sfGFP-LVAtag expression cassette under the control of pTacI promoter |
© 2021 • iGEM Evry Paris-Saclay