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| <div class="modal-content"> | | <div class="modal-content"> |
| <div class="modal-header"><h5 class="modal-title" id="Proof2Label">Proof2</h5></div> | | <div class="modal-header"><h5 class="modal-title" id="Proof2Label">Proof2</h5></div> |
− | <div class="modal-body"><h1>Chassis Yeast</h1> | + | <div class="modal-body"><h2>Chassis Yeast</h2> |
| <p>Pichia pastoris, a kind of methylotrophic yeast separated by Ogata in 1969, can grow in culture | | <p>Pichia pastoris, a kind of methylotrophic yeast separated by Ogata in 1969, can grow in culture |
| media with methanol as the only carbon source.[1].P.pastoris, being the second widely used | | media with methanol as the only carbon source.[1].P.pastoris, being the second widely used |
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| proteins have been successfully expressed in P. pastoris, and P. pastoris has become one of the | | proteins have been successfully expressed in P. pastoris, and P. pastoris has become one of the |
| most commonly used expression systems.[3].</p> | | most commonly used expression systems.[3].</p> |
− | <h1>Xylose Responding Regulation System</h1> | + | <h2>Xylose Responding Regulation System</h2> |
| <p>In order to express the genes at the right time, we introduced a Xylose Responding Regulation | | <p>In order to express the genes at the right time, we introduced a Xylose Responding Regulation |
| System. The system includes two promoters: the constitutive promoter Panb1, applied in the dye | | System. The system includes two promoters: the constitutive promoter Panb1, applied in the dye |
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| Pynr071c[5], applied in the decolorization and straightening devices. And the two promoters have | | Pynr071c[5], applied in the decolorization and straightening devices. And the two promoters have |
| been proved that would express in Saccharomyces cerevisiae, so we try them in P.pastoris.</p> | | been proved that would express in Saccharomyces cerevisiae, so we try them in P.pastoris.</p> |
− | <h1>Expression of parts</h1> | + | <h2>Expression of parts</h2> |
| <p>As for our functional genes, they had been expressed in some chassis. FMO <a | | <p>As for our functional genes, they had been expressed in some chassis. FMO <a |
| href="http://parts.igem.org/Part:BBa_K3711003">BBa_K3711003</a> in E.coli, Laccase <a | | href="http://parts.igem.org/Part:BBa_K3711003">BBa_K3711003</a> in E.coli, Laccase <a |
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| <div class="modal-content"> | | <div class="modal-content"> |
| <div class="modal-header"><h5 class="modal-title" id="Proof3Label">Proof3</h5></div> | | <div class="modal-header"><h5 class="modal-title" id="Proof3Label">Proof3</h5></div> |
− | <div class="modal-body"><h1>The pigments can be used to dye</h1> | + | <div class="modal-body"><h2>The pigments can be used to dye</h2> |
| <div class="image"><img alt=" " | | <div class="image"><img alt=" " |
| src="https://static.igem.org/mediawiki/2021/d/d7/T--HUST-China--img--proof--proof2.png" | | src="https://static.igem.org/mediawiki/2021/d/d7/T--HUST-China--img--proof--proof2.png" |
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| href="https://2021.igem.org/Team:HUST-China/Proof_Of_Concept/Results">result</a> for | | href="https://2021.igem.org/Team:HUST-China/Proof_Of_Concept/Results">result</a> for |
| more information.</p> | | more information.</p> |
− | <h1>The Color of Hairs Can Be Faded</h1> | + | <h2>The Color of Hairs Can Be Faded</h2> |
| <p>In our design, we have gained information from papers that lipoxygenase can fade the color | | <p>In our design, we have gained information from papers that lipoxygenase can fade the color |
| produced by lycopene[11], curcumin reductase can fade curcumin[12], and indigo can be faded by | | produced by lycopene[11], curcumin reductase can fade curcumin[12], and indigo can be faded by |
| laccase[13]. We also finished some experiments to prove these enzymes can work as we thought. <a | | laccase[13]. We also finished some experiments to prove these enzymes can work as we thought. <a |
| href="https://2021.igem.org/Team:HUST-China/Proof_Of_Concept/Results">(Results)</a></p> | | href="https://2021.igem.org/Team:HUST-China/Proof_Of_Concept/Results">(Results)</a></p> |
− | <h1>The Short Peptides Can Be Used to Perm</h1> | + | <h2>The Short Peptides Can Be Used to Perm</h2> |
| <div class="image"><img alt=" " | | <div class="image"><img alt=" " |
| src="https://static.igem.org/mediawiki/2021/a/ab/T--HUST-China--img--proof--proof3.png" | | src="https://static.igem.org/mediawiki/2021/a/ab/T--HUST-China--img--proof--proof3.png" |
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| it and more information please refer to <a | | it and more information please refer to <a |
| href="https://2021.igem.org/Team:HUST-China/Proof_Of_Concept/Results">result</a>.</p> | | href="https://2021.igem.org/Team:HUST-China/Proof_Of_Concept/Results">result</a>.</p> |
− | <h1>Use Our Products in Real Life</h1> | + | <h2>Use Our Products in Real Life</h2> |
| <p>For the use of our products in reality, we have designed two main apparatuses. The fermentation | | <p>For the use of our products in reality, we have designed two main apparatuses. The fermentation |
| bottle is used to produce pigments and short peptides. And the dyeing comb is used to change the | | bottle is used to produce pigments and short peptides. And the dyeing comb is used to change the |