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| href="https://2021.igem.org/Team:HUST-China/Judging">Judging</a><a | | href="https://2021.igem.org/Team:HUST-China/Judging">Judging</a><a |
| class="dropdown-item" | | class="dropdown-item" |
− | href="https://video.igem.org/w/aMwNT6eBryGs8xG5avXD51">Promotion | + | href="https://2021.igem.org/Team:HUST-China/https:/video.igem.org/w/aMwNT6eBryGs8xG5avXD51">Promotion |
| Video</a><a class="dropdown-item" | | Video</a><a class="dropdown-item" |
− | href="https://igem.org/2021_Judging_Form?id=3711">Judging | + | href="https://2021.igem.org/Team:HUST-China/https:/igem.org/2021_Judging_Form?id=3711">Judging |
| Form</a></div> | | Form</a></div> |
| </li> | | </li> |
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| <article><h1>Introduction</h1> | | <article><h1>Introduction</h1> |
| <p>Our project (click <a | | <p>Our project (click <a |
− | href="https://2021.igem.org/Team:HUST-China/Design">design</a> for detailed | + | href="https://2021.igem.org/Team:HUST-China/Proof_Of_Concept/Design">design</a> for detailed |
| information) integrated at most five reactions within a system. Each reaction in the system is | | information) integrated at most five reactions within a system. Each reaction in the system is |
| interrelated by metabolic or regulatory pathways. Integrated with the systems, the Pichia | | interrelated by metabolic or regulatory pathways. Integrated with the systems, the Pichia |
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| <div class="modal-content"> | | <div class="modal-content"> |
| <div class="modal-header"><h5 class="modal-title" id="Proof1Label">Proof1</h5></div> | | <div class="modal-header"><h5 class="modal-title" id="Proof1Label">Proof1</h5></div> |
− | <div class="modal-body"></div> | + | <div class="modal-body"><p>In the initial stage of construction, we adapted seamless cloning of homologous recombination. |
| + | Unique homologous sequences at front and rear ends of joining parts were designed based on |
| + | Gibson assembly seamless cloning principle. After PCR, the parts underwent recombination |
| + | reaction in the recombination enzyme system from some companies. Unfortunately, the products of |
| + | ligation fell short of our expectation and we hardly found any explanation to this. Later, the |
| + | restricted enzyme and T4 ligation enzyme were used to construct the devices, which is a less |
| + | efficient way of construction. Most devices and a few systems were successfully constructed and |
| + | we will continue to complete our work in the future. More details please click <a |
| + | href="https://2021.igem.org/Team:HUST-China/Proof_Of_Concept/Engineering">here</a></p></div> |
| <div class="modal-footer"> | | <div class="modal-footer"> |
| <button class="btn btn-danger" data-dismiss="modal" type="button">Close</button> | | <button class="btn btn-danger" data-dismiss="modal" type="button">Close</button> |
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| </div> | | </div> |
| </div> | | </div> |
− | <p>In the initial stage of construction, we adapted seamless cloning of homologous recombination.
| |
− | Unique homologous sequences at front and rear ends of joining parts were designed based on
| |
− | Gibson assembly seamless cloning principle. After PCR, the parts underwent recombination
| |
− | reaction in the recombination enzyme system from some companies. Unfortunately, the products of
| |
− | ligation fell short of our expectation and we hardly found any explanation to this. Later, the
| |
− | restricted enzyme and T4 ligation enzyme were used to construct the devices, which is a less
| |
− | efficient way of construction. Most devices and a few systems were successfully constructed and
| |
− | we will continue to complete our work in the future. More details please click <a
| |
− | href="https://2021.igem.org/Team:HUST-China/Engineering">here</a></p>
| |
| <h1>Expression</h1> | | <h1>Expression</h1> |
| <p>The constructed devices and systems were transformed into pichia pastoris GS115 and expressed. We | | <p>The constructed devices and systems were transformed into pichia pastoris GS115 and expressed. We |
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| <div class="modal-content"> | | <div class="modal-content"> |
| <div class="modal-header"><h5 class="modal-title" id="Proof2Label">Proof2</h5></div> | | <div class="modal-header"><h5 class="modal-title" id="Proof2Label">Proof2</h5></div> |
− | <div class="modal-body"></div> | + | <div class="modal-body"><h1>Chassis Yeast</h1> |
− | <div class="modal-footer">
| + | |
− | <button class="btn btn-danger" data-dismiss="modal" type="button">Close</button>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <h1>Chassis Yeast</h1>
| + | |
| <p>Pichia pastoris, a kind of methylotrophic yeast separated by Ogata in 1969, can grow in culture | | <p>Pichia pastoris, a kind of methylotrophic yeast separated by Ogata in 1969, can grow in culture |
| media with methanol as the only carbon source.[1].P.pastoris, being the second widely used | | media with methanol as the only carbon source.[1].P.pastoris, being the second widely used |
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| href="http://parts.igem.org/Part:BBa_K3711011">BBa_K3711011</a> in E.coli, curA <a | | href="http://parts.igem.org/Part:BBa_K3711011">BBa_K3711011</a> in E.coli, curA <a |
| href="http://parts.igem.org/Part:BBa_K3711012">BBa_K3711012</a> in P.pastoris[8]. So we try | | href="http://parts.igem.org/Part:BBa_K3711012">BBa_K3711012</a> in P.pastoris[8]. So we try |
− | to express them in eukaryotic system P.pastoris GS115.</p> | + | to express them in eukaryotic system P.pastoris GS115.</p></div> |
| + | <div class="modal-footer"> |
| + | <button class="btn btn-danger" data-dismiss="modal" type="button">Close</button> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | </div> |
| + | </div> |
| <h1>Application</h1> | | <h1>Application</h1> |
| <p>When it comes to the application of our products, we should ensure the theoretical basis of our | | <p>When it comes to the application of our products, we should ensure the theoretical basis of our |
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| </button> | | </button> |
| <div aria-hidden="true" aria-labelledby="Proof3Label" class="modal fade" data-backdrop="static" | | <div aria-hidden="true" aria-labelledby="Proof3Label" class="modal fade" data-backdrop="static" |
− | data-keyboard="false" id="Perm" tabindex="-1"> | + | data-keyboard="false" id="Proof3" tabindex="-1"> |
| <div class="modal-dialog modal-dialog-scrollable modal-dialog-centered modal-xl"> | | <div class="modal-dialog modal-dialog-scrollable modal-dialog-centered modal-xl"> |
| <div class="modal-content"> | | <div class="modal-content"> |
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| researched a lot about dye with the three pigments. We dyed the hair successfully and compared | | researched a lot about dye with the three pigments. We dyed the hair successfully and compared |
| it with the hair dyed with the traditional chemical way. Please refer to <a | | it with the hair dyed with the traditional chemical way. Please refer to <a |
− | href="https://2021.igem.org/Team:HUST-China/Results">result</a> for | + | href="https://2021.igem.org/Team:HUST-China/Proof_Of_Concept/Results">result</a> for |
| more information.</p> | | more information.</p> |
| <h1>The Color of Hairs Can Be Faded</h1> | | <h1>The Color of Hairs Can Be Faded</h1> |
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| produced by lycopene[11], curcumin reductase can fade curcumin[12], and indigo can be faded by | | produced by lycopene[11], curcumin reductase can fade curcumin[12], and indigo can be faded by |
| laccase[13]. We also finished some experiments to prove these enzymes can work as we thought. <a | | laccase[13]. We also finished some experiments to prove these enzymes can work as we thought. <a |
− | href="https://2021.igem.org/Team:HUST-China/Results">(Results)</a></p> | + | href="https://2021.igem.org/Team:HUST-China/Proof_Of_Concept/Results">(Results)</a></p> |
| <h1>The Short Peptides Can Be Used to Perm</h1> | | <h1>The Short Peptides Can Be Used to Perm</h1> |
| <div class="image"><img alt=" " | | <div class="image"><img alt=" " |
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| with α-keratins, thus changing the shape of hair[14]. We also designed some experiments to prove | | with α-keratins, thus changing the shape of hair[14]. We also designed some experiments to prove |
| it and more information please refer to <a | | it and more information please refer to <a |
− | href="https://2021.igem.org/Team:HUST-China/Results">result</a>.</p> | + | href="https://2021.igem.org/Team:HUST-China/Proof_Of_Concept/Results">result</a>.</p> |
| <h1>Use Our Products in Real Life</h1> | | <h1>Use Our Products in Real Life</h1> |
| <p>For the use of our products in reality, we have designed two main apparatuses. The fermentation | | <p>For the use of our products in reality, we have designed two main apparatuses. The fermentation |
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| color and shape of your hairs. With the help of them, you can finish the process of perming and | | color and shape of your hairs. With the help of them, you can finish the process of perming and |
| dyeing. Please refer to <a | | dyeing. Please refer to <a |
− | href="https://2021.igem.org/Team:HUST-China/Hardware">Hardware</a> for | + | href="https://2021.igem.org/Team:HUST-China/Proof_Of_Concept/Hardware">Hardware</a> for |
| more information.</p> | | more information.</p> |
| <div class="row g-0"> | | <div class="row g-0"> |
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| <div class="dialogbox"><p>Hmm…I can hardly pick out any flaw in this part, what about you? | | <div class="dialogbox"><p>Hmm…I can hardly pick out any flaw in this part, what about you? |
| Here is another thing I really care about—the <a | | Here is another thing I really care about—the <a |
− | href="https://2021.igem.org/Team:HUST-China/Safety">safety </a>of | + | href="https://2021.igem.org/Team:HUST-China/Proof_Of_Concept/Safety">safety </a>of |
| the products! Don’t you worry about whether the products of this team can cause cancer | | the products! Don’t you worry about whether the products of this team can cause cancer |
| or do damage to hair like other hairdressing products in the market?</p></div> | | or do damage to hair like other hairdressing products in the market?</p></div> |