Difference between revisions of "Team:HUST-China/Parts"

(Prototype team page)
 
Line 1: Line 1:
−
{{IGEM_TopBar}}
+
<!-- # TODO: #6 Fix table caption font--><!-- # TODO: #7 Fix citations links font size--><html lang="en"><head><meta charset="utf-8"/><meta content="width=device-width,initial-scale=1" name="viewport"/><link href="https://2021.igem.org/Template:HUST-China/css/contentCSS?action=raw&amp;ctype=text/css" rel="stylesheet"/><title>Parts | iGEM HUST-China</title><link href="https://2021.igem.org/Template:HUST-China/css/contentCSS?action=raw&amp;ctype=text/css" rel="stylesheet"/></head><body><!-- # TODO: #6 Fix table caption font--><!-- # TODO: #7 Fix citations links font size--><nav class="navbar navbar-expand-xl fixed-top"><div class="container d-flex justify-content-between"><a class="navbar-brand" href="https://2021.igem.org/Team:HUST-China"><i class="navbar-logo-left"></i><span>HUST-China</span></a><button aria-controls="navbarNav" aria-expanded="false" aria-label="Toggle navigation" class="navbar-toggler" data-target="#navbarNav" data-toggle="collapse" type="button"><span class="navbar-toggler-icon"></span></button><div class="collapse navbar-collapse" id="navbarNav"><ul class="navbar-nav ml-auto"><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarProjectDropdown" role="button">Project</a><div aria-labelledby="navbarProjectDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Description">Description</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Design">Design</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Proof_Of_Concept">Proof of Concept</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Safety">Safety</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Implementation">Proposed Implementation</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Contribution">Contribution</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarHuman PracticesDropdown" role="button">Human Practices</a><div aria-labelledby="navbarHuman PracticesDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Human_Practices">Overview</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Human_Practices_01">Part 1</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Human_Practices_02">Part 2</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Collaborations">Part 3</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Education">Part 4</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarModelingDropdown" role="button">Modeling</a><div aria-labelledby="navbarModelingDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Model">Overview</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Gene_Pathway">Gene Pathway</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Dye_color_forecast">Dye color forecast</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Micronucleus_Counter">Micronucleus Counter</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarProductsDropdown" role="button">Products</a><div aria-labelledby="navbarProductsDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Products">Overview</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Software">Software</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Hardware">Hardware</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Another_Area">Another Area</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarExperimentsDropdown" role="button">Experiments</a><div aria-labelledby="navbarExperimentsDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Experiments">Overview</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Parts">Parts</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Engineering">Engineering Success</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Results">Results</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Protocol">Protocol</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Notebook">Notebook</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarPeopleDropdown" role="button">People</a><div aria-labelledby="navbarPeopleDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Team">Team</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Partnership">Partnership</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Attributions">Attributions</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarAchievementsDropdown" role="button">Achievements</a><div aria-labelledby="navbarAchievementsDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/Judging">Judging</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/https:/video.igem.org/w/aMwNT6eBryGs8xG5avXD51">Promotion Video</a><a class="dropdown-item" href="https://2021.igem.org/Team:HUST-China/https:/igem.org/2021_Judging_Form?id=3711">Judging Form</a></div></li></ul></div></div></nav><div class="navbar-extra fixed-top"></div><header class="d-flex justify-content-center align-items-center"><div class="container"><h1 id="Top">Parts</h1><p class="lead pl-1"></p><hr class="my-4"/></div></header><main><div class="container"><div class="row"><div class="sidebar col-lg-3"><div class="nav" id="contents"><h5>Contents</h5><ul></ul></div></div><div class="content col-lg-9"><article><h1>Introduction</h1><p>Biology is different from other engineering fields due to a lack of modular nature of the structure and function, which means the expression of gene will change when transferred from one species to another. What synthetic biology is dedicated to is the modularization and standardization of functional gene, to make the expression of it predictable in different chassis organism. Our project has registered 19 basic parts and 56 composite parts, all qualified for standardized requirement of RFC[10]. We have also tested these parts. We hope that everyone can utilize our parts based on our research through registry and that the information for parts can further be completed so that more people can make use of them.</p><h1>Basic parts</h1><table><thead><tr><th>Part</th><th>Number</th><th>Part Name</th><th>Type</th><th>length</th></tr></thead><tbody><tr><td>BBa_K3711019</td><td>Panb1</td><td>Regulatory</td><td>399</td><td></td></tr><tr><td>BBa_K3711001</td><td>Pynr071c</td><td>Regulatory</td><td>1188</td><td></td></tr><tr><td>BBa_K3711015</td><td>AOX1</td><td>Regulatory</td><td>937</td><td></td></tr><tr><td>BBa_K3711017</td><td>AOX1 terminator</td><td>Terminator</td><td>247</td><td></td></tr><tr><td>BBa_K3711002</td><td>ROX1</td><td>Coding</td><td>1251</td><td></td></tr><tr><td>BBa_K3711003</td><td>FMO</td><td>Coding</td><td>1395</td><td></td></tr><tr><td>BBa_K3711004</td><td>Laccase</td><td>Coding</td><td>1602</td><td></td></tr><tr><td>BBa_K3711005</td><td>crtE</td><td>Coding</td><td>927</td><td></td></tr><tr><td>BBa_K3711006</td><td>crtB</td><td>Coding</td><td>948</td><td></td></tr><tr><td>BBa_K3711007</td><td>crtI</td><td>Coding</td><td>1497</td><td></td></tr><tr><td>BBa_K3711008</td><td>LOX2</td><td>Coding</td><td>2709</td><td></td></tr><tr><td>BBa_K3711009</td><td>4CL</td><td>Coding</td><td>1704</td><td></td></tr><tr><td>BBa_K3711010</td><td>ACC</td><td>Coding</td><td>1800</td><td></td></tr><tr><td>BBa_K3711011</td><td>CUS</td><td>Coding</td><td>1227</td><td></td></tr><tr><td>BBa_K3711012</td><td>curA</td><td>Coding</td><td>1056</td><td></td></tr><tr><td>BBa_K3711013</td><td>PepACS</td><td>Coding</td><td>168</td><td></td></tr><tr><td>BBa_K3711014</td><td>DsbC</td><td>Coding</td><td>903</td><td></td></tr><tr><td>BBa_K3711016</td><td>α-factor</td><td>Coding</td><td>267</td><td></td></tr><tr><td>BBa_K3711018</td><td>FMO dimer</td><td>Coding</td><td>2769</td><td></td></tr></tbody></table><h1>Improvement</h1><p>Flaving Containing-Monooxygenase (FMO) is an enzyme that can be used to produce indigo. This basic part has been registered by iGEM13_Berkeley as BBa_K1131000. We improved its function and registered as a new part BBa_K3711018.</p><p>Firstly, we optimized the codon of this gene according to the codon preference of P.pastoris to improve its expression level.</p><p>Secondly, research shows that FAD is the prosthetic group of FMO, NADPH is the cofactor of FMO. FMO-FAD-NADPH complex usually forms a asymmetric dimer as a unit to work. The enzyme-substrate complex formed when enzyme acts on substrate, has two dimers per unit[1].</p><p>So, we wanted to construct the two FMO fusion proteins named FMO dimer and expressed it, hoping that they could form a dimer more easily and improve their activity.</p><p>Then, we predicted the 3D structure of FMO dimer. The result shows that the structure could be formed and the dimer could combinate with substrate.</p><p>Therefore, we started to build FMO dimer and achieved success in expression. More details please click here.</p><h1>Composite parts</h1><table><thead><tr><th>Part</th><th>Number</th><th>Part Name</th><th>Type</th><th>length</th></tr></thead><tbody><tr><td>BBa_K3711039</td><td>AOX1-α factor-ROX1-AOX1 Terminator</td><td>Composite</td><td>2722</td><td></td></tr><tr><td>BBa_K3711040</td><td>AOX1-α factor-FMO-AOX1 Terminator</td><td>Composite</td><td>2866</td><td></td></tr><tr><td>BBa_K3711041</td><td>AOX1-α factor-Laccase-AOX1 Terminator</td><td>Composite</td><td>3073</td><td></td></tr><tr><td>BBa_K3711042</td><td>AOX1-α factor-crtE-AOX1 Terminator</td><td>Composite</td><td>2398</td><td></td></tr><tr><td>BBa_K3711043</td><td>AOX1-α factor-crtB-AOX1 Terminator</td><td>Composite</td><td>2419</td><td></td></tr><tr><td>BBa_K3711044</td><td>AOX1-α factor-crtI-AOX1 Terminator</td><td>Composite</td><td>2968</td><td></td></tr><tr><td>BBa_K3711045</td><td>AOX1-α factor-LOX2-AOX1 Terminator</td><td>Composite</td><td>4180</td><td></td></tr><tr><td>BBa_K3711046</td><td>AOX1-α factor-4CL-AOX1 Terminator</td><td>Composite</td><td>3175</td><td></td></tr><tr><td>BBa_K3711047</td><td>AOX1-α factor-ACC-AOX1 Terminator</td><td>Composite</td><td>3271</td><td></td></tr><tr><td>BBa_K3711048</td><td>AOX1-α factor-CUS-AOX1 Terminator</td><td>Composite</td><td>2698</td><td></td></tr><tr><td>BBa_K3711049</td><td>AOX1-α factor-curA-AOX1 Terminator</td><td>Composite</td><td>2527</td><td></td></tr><tr><td>BBa_K3711050</td><td>AOX1-α factor-PepACS-AOX1 Terminator</td><td>Composite</td><td>1639</td><td></td></tr><tr><td>BBa_K3711051</td><td>AOX1-α factor-DsbC-AOX1 Terminator</td><td>Composite</td><td>2374</td><td></td></tr><tr><td>BBa_K3711052</td><td>Pynr071c-α factor-FMO dimer-AOX1 Terminator</td><td>Composite</td><td>4491</td><td></td></tr><tr><td>BBa_K3711053</td><td>Pynr071c-α factor-ROX1-AOX1 Terminator</td><td>Composite</td><td>2973</td><td></td></tr><tr><td>BBa_K3711054</td><td>Panb1-α factor-FMO-AOX1 Terminator</td><td>Composite</td><td>2328</td><td></td></tr><tr><td>BBa_K3711055</td><td>Pynr071c-α factor-Laccase-AOX1 Terminator</td><td>Composite</td><td>3324</td><td></td></tr><tr><td>BBa_K3711056</td><td>Panb1-α factor-crtE-AOX1 Terminator</td><td>Composite</td><td>1860</td><td></td></tr><tr><td>BBa_K3711057</td><td>Panb1-α factor-crtB-AOX1 Terminator</td><td>Composite</td><td>1881</td><td></td></tr><tr><td>BBa_K3711058</td><td>Panb1-α factor-crtI-AOX1 Terminator</td><td>Composite</td><td>2430</td><td></td></tr><tr><td>BBa_K3711060</td><td>Pynr071c-α factor-LOX2-AOX1 Terminator</td><td>Composite</td><td>4431</td><td></td></tr><tr><td>BBa_K3711061</td><td>Panb1-α factor-4CL-AOX1 Terminator</td><td>Composite</td><td>2637</td><td></td></tr><tr><td>BBa_K3711062</td><td>Panb1-α factor-ACC-AOX1 Terminator</td><td>Composite</td><td>2733</td><td></td></tr><tr><td>BBa_K3711063</td><td>Panb1-α factor-CUS-AOX1 Terminator</td><td>Composite</td><td>2160</td><td></td></tr><tr><td>BBa_K3711064</td><td>Pynr071c-α factor-curA-AOX1 Terminator</td><td>Composite</td><td>2778</td><td></td></tr><tr><td>BBa_K3711065</td><td>Panb1-α factor-PepACS-AOX1 Terminator</td><td>Composite</td><td>1101</td><td></td></tr><tr><td>BBa_K3711066</td><td>Pynr071c-α factor-DsbC-AOX1 Terminator</td><td>Composite</td><td>2625</td><td></td></tr><tr><td>BBa_K3711067</td><td>Pynr071c-ROX1-AOX1 Terminator</td><td>Composite</td><td>2700</td><td></td></tr><tr><td>BBa_K3711068</td><td>Panb1-FMO-AOX1 Terminator</td><td>Composite</td><td>2055</td><td></td></tr><tr><td>BBa_K3711069</td><td>Pynr071c-Laccase-AOX1 Terminator</td><td>Composite</td><td>3051</td><td></td></tr><tr><td>BBa_K3711070</td><td>Panb1-crtE-AOX1 Terminator</td><td>Composite</td><td>1587</td><td></td></tr><tr><td>BBa_K3711071</td><td>Panb1-crtB-AOX1 Terminator</td><td>Composite</td><td>1608</td><td></td></tr><tr><td>BBa_K3711072</td><td>Panb1-crtI-AOX1 Terminator</td><td>Composite</td><td>2157</td><td></td></tr><tr><td>BBa_K3711073</td><td>Pynr071c-LOX2-AOX1 Terminator</td><td>Composite</td><td>4158</td><td></td></tr><tr><td>BBa_K3711074</td><td>Panb1-4CL-AOX1 Terminator</td><td>Composite</td><td>2364</td><td></td></tr><tr><td>BBa_K3711075</td><td>Panb1-ACC-AOX1 Terminator</td><td>Composite</td><td>2460</td><td></td></tr><tr><td>BBa_K3711076</td><td>Panb1-CUS-AOX1 Terminator</td><td>Composite</td><td>1887</td><td></td></tr><tr><td>BBa_K3711077</td><td>Pynr071c-curA-AOX1 Terminator</td><td>Composite</td><td>2505</td><td></td></tr><tr><td>BBa_K3711078</td><td>Panb1-PepACS-AOX1 Terminator</td><td>Composite</td><td>828</td><td></td></tr><tr><td>BBa_K3711079</td><td>Pynr071c-DsbC-AOX1 Terminator</td><td>Composite</td><td>2352</td><td></td></tr><tr><td>BBa_K3711080</td><td>AOX1-α factor-Lycopene-AOX1 Terminator</td><td>Composite</td><td>7801</td><td></td></tr><tr><td>BBa_K3711081</td><td>AOX1-α factor-Curcumin-AOX1 Terminator</td><td>Composite</td><td>9160</td><td></td></tr><tr><td>BBa_K3711082</td><td>Panb1-α factor-Curcumin-AOX1 Terminator</td><td>Composite</td><td>7546</td><td></td></tr><tr><td>BBa_K3711083</td><td>Panb1-α factor-Lycopene-AOX1 Terminator</td><td>Composite</td><td>6187</td><td></td></tr><tr><td>BBa_K3711084</td><td>Panb1-Curcumin-AOX1 Terminator</td><td>Composite</td><td>6727</td><td></td></tr><tr><td>BBa_K3711085</td><td>Panb1-Lycopene-AOX1 Terminator</td><td>Composite</td><td>5368</td><td></td></tr><tr><td>BBa_K3711086</td><td>The synthesis and decolorization system of indigo</td><td>Composite</td><td>8368</td><td></td></tr><tr><td>BBa_K3711087</td><td>The synthesis and decolorization system of indigo</td><td>Composite</td><td>7822</td><td></td></tr><tr><td>BBa_K3711088</td><td>The synthesis and decolorization system of indigo</td><td>Composite</td><td>14948</td><td></td></tr><tr><td>BBa_K3711089</td><td>The synthesis and decolorization system of lycopene without signal peptide</td><td>Composite</td><td>12242</td><td></td></tr><tr><td>BBa_K3711090</td><td>The synthesis and decolorization system of lycopene without signal peptide</td><td>Composite</td><td>13040</td><td></td></tr><tr><td>BBa_K3711091</td><td>The synthesis and decolorization system of curcumin without signal peptide</td><td>Composite</td><td>11948</td><td></td></tr><tr><td>BBa_K3711092</td><td>The perm and straightening system</td><td>Composite</td><td>6442</td><td></td></tr><tr><td>BBa_K3711093</td><td>The perm and straightening system without signal peptide</td><td>Composite</td><td>5896</td><td></td></tr></tbody></table><div class="row g-0"><div class="col-4 girl"><img src="https://static.igem.org/mediawiki/2021/2/28/T--HUST-China--img--girl.png"/></div><div class="col-5"><div class="dialogbox"><p>How ingenious! It seems that constructing composite parts and full pathway takes <a href="https://2021.igem.org/Team:HUST-China/Parts/Engineering">engineering</a> wisdom.</p></div></div></div></article></div></div></div></main><footer><div class="container"><div class="row"><div class="col-7"><div class="row justify-content-center"><div class="footer-logo"></div></div><p><span>CONTACT US: </span><a href="mailto:iGEMHUSTChina@163.com">iGEMHUSTChina@163.com</a></p><p>Huazhong University of Sci. &amp; Tech., Wuhan, China</p><p>1037# Luoyu Rd, Wuhan, P.R.China 430074</p><p>Copyright © <span>HUST-China </span>iGEM 2021</p></div><div class="col-5"><div class="row"><div class="footer-xmind"></div></div><div class="row"><div class="footer-snapgene"></div></div><div class="row"><div class="footer-NEBI"></div></div></div></div></div></footer><script src="https://2021.igem.org/Template:HUST-China/content-bundleJS?action=raw&amp;ctype=text/javascript"></script><script src="https://2021.igem.org/Template:HUST-China/mathjax-bundleJS?action=raw&amp;ctype=text/javascript"></script></body></html>
−
{{HUST-China}}
+
−
<html>
+
−
 
+
−
 
+
−
<div class="column full_size">
+
−
<h1>Parts</h1>
+
−
<p>Each team will make new parts during iGEM and will add them to the Registry of Standard Biological Parts. iGEM provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
+
−
<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
+
−
</div>
+
−
 
+
−
<div class="column full_size">
+
−
<div class="highlight decoration_background">
+
−
<h3>Note</h3>
+
−
<p>Note that parts must be well documented on each Part's Main Page on the <a href="http://parts.igem.org/Main_Page">Registry</a>. This documentation includes all of the characterization data for your parts. <b>The part's data MUST be on the part's Main Page on the Registry for your team to be eligible for medals and special prizes pertaining to parts.</b> <br><br>
+
−
This page serves to <i>showcase</i> the parts you have made and should include links to the Registry pages for your parts. Future teams and other users are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
+
−
</div>
+
−
</div>
+
−
 
+
−
<div class="clear extra_space"></div>
+
−
<div class="line_divider"></div>
+
−
<div class="clear extra_space"></div>
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
−
<div class="column full_size">
+
−
<div class="highlight decoration_B_full">
+
−
 
+
−
<h3>Adding parts to the Registry</h3>
+
−
<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
+
−
 
+
−
<p>We encourage teams to start adding and documenting their parts on the Registry as soon as they can. Once you add your parts to the Registry, you can continue to add documentation to them throughout the iGEM season (up until the Registry freeze). This will allow you to remember all the details about your parts and store their history in the wiki. Documentation includes the characterization data of your parts.</p>
+
−
<div class="button_link">
+
−
<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
+
−
ADD PARTS
+
−
</a>
+
−
</div>
+
−
 
+
−
</div>
+
−
</div>
+
−
 
+
−
 
+
−
 
+
−
<div class="column third_size">
+
−
<div class="highlight decoration_A_full">
+
−
<h3>Inspiration: Basic Parts</h3>
+
−
<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
+
−
 
+
−
<p> You can also take a look at the following examples for Basic Parts on the Registry:</p>
+
−
<ul>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K3114006">2019 Calgary </a></li>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K3027000">2019 GO Paris Saclay</a></li>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K3187028">2019 TU Darmstadt</a></li>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K3552000">2020 Links China</a></li>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K3558000">2020 UNSW Australia</a></li>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K3338002">2020 Hannover</a></li>
+
−
</ul>
+
−
</div>
+
−
</div>
+
−
 
+
−
<div class="column third_size">
+
−
<div class="highlight decoration_A_full">
+
−
<h3>Inspiration: Composite Parts</h3>
+
−
<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
+
−
 
+
−
<p> You can also take a look at the following examples for Composite Parts on the Registry:</p>
+
−
<ul>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K3198007">2019 NUS Singapore </a></li>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K2932003">2019 Mingdao</a></li>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K2980009">2019 Tsinghua</a></li>
+
−
 
+
−
<li><a href="http://parts.igem.org/Part:BBa_K3407022">2020 TUDelft</a></li>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K3380500">2020 Edinburgh</a></li>
+
−
<li><a href="http://parts.igem.org/Part:BBa_K3512042">2020 BITSPilani Goa India</a></li>
+
−
</ul>
+
−
</div>
+
−
</div>
+
−
 
+
−
 
+
−
 
+
−
<div class="column third_size">
+
−
 
+
−
<h3>What information do I need to start putting my parts on the Registry?</h3>
+
−
<p>The information needed to initially create a part on the Registry is:</p>
+
−
<ul>
+
−
<li>Part Name</li>
+
−
<li>Part type</li>
+
−
<li>Creator</li>
+
−
<li>Sequence</li>
+
−
<li>Short Description (60 characters on what the DNA does)</li>
+
−
<li>Long Description (Longer description of what the DNA does)</li>
+
−
<li>Design considerations</li>
+
−
</ul>
+
−
 
+
−
<p>
+
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, you must also put it up on the part page. </p>
+
−
 
+
−
</div>
+
−
 
+
−
 
+
−
<div class="clear extra_space"></div>
+
−
<div class="line_divider"></div>
+
−
<div class="clear extra_space"></div>
+
−
 
+
−
<div class="column full_size">
+
−
<h3>Part Table </h3>
+
−
 
+
−
<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
+
−
 
+
−
</html>
+
−
<groupparts>iGEM20 HUST-China</groupparts>
+
−
<html>
+
−
</div>
+
−
 
+
−
 
+
−
 
+
−
 
+
−
</html>
+

Revision as of 23:43, 21 October 2021

Parts | iGEM HUST-China

Parts

Introduction

Biology is different from other engineering fields due to a lack of modular nature of the structure and function, which means the expression of gene will change when transferred from one species to another. What synthetic biology is dedicated to is the modularization and standardization of functional gene, to make the expression of it predictable in different chassis organism. Our project has registered 19 basic parts and 56 composite parts, all qualified for standardized requirement of RFC[10]. We have also tested these parts. We hope that everyone can utilize our parts based on our research through registry and that the information for parts can further be completed so that more people can make use of them.

Basic parts

PartNumberPart NameTypelength
BBa_K3711019Panb1Regulatory399
BBa_K3711001Pynr071cRegulatory1188
BBa_K3711015AOX1Regulatory937
BBa_K3711017AOX1 terminatorTerminator247
BBa_K3711002ROX1Coding1251
BBa_K3711003FMOCoding1395
BBa_K3711004LaccaseCoding1602
BBa_K3711005crtECoding927
BBa_K3711006crtBCoding948
BBa_K3711007crtICoding1497
BBa_K3711008LOX2Coding2709
BBa_K37110094CLCoding1704
BBa_K3711010ACCCoding1800
BBa_K3711011CUSCoding1227
BBa_K3711012curACoding1056
BBa_K3711013PepACSCoding168
BBa_K3711014DsbCCoding903
BBa_K3711016α-factorCoding267
BBa_K3711018FMO dimerCoding2769

Improvement

Flaving Containing-Monooxygenase (FMO) is an enzyme that can be used to produce indigo. This basic part has been registered by iGEM13_Berkeley as BBa_K1131000. We improved its function and registered as a new part BBa_K3711018.

Firstly, we optimized the codon of this gene according to the codon preference of P.pastoris to improve its expression level.

Secondly, research shows that FAD is the prosthetic group of FMO, NADPH is the cofactor of FMO. FMO-FAD-NADPH complex usually forms a asymmetric dimer as a unit to work. The enzyme-substrate complex formed when enzyme acts on substrate, has two dimers per unit[1].

So, we wanted to construct the two FMO fusion proteins named FMO dimer and expressed it, hoping that they could form a dimer more easily and improve their activity.

Then, we predicted the 3D structure of FMO dimer. The result shows that the structure could be formed and the dimer could combinate with substrate.

Therefore, we started to build FMO dimer and achieved success in expression. More details please click here.

Composite parts

PartNumberPart NameTypelength
BBa_K3711039AOX1-α factor-ROX1-AOX1 TerminatorComposite2722
BBa_K3711040AOX1-α factor-FMO-AOX1 TerminatorComposite2866
BBa_K3711041AOX1-α factor-Laccase-AOX1 TerminatorComposite3073
BBa_K3711042AOX1-α factor-crtE-AOX1 TerminatorComposite2398
BBa_K3711043AOX1-α factor-crtB-AOX1 TerminatorComposite2419
BBa_K3711044AOX1-α factor-crtI-AOX1 TerminatorComposite2968
BBa_K3711045AOX1-α factor-LOX2-AOX1 TerminatorComposite4180
BBa_K3711046AOX1-α factor-4CL-AOX1 TerminatorComposite3175
BBa_K3711047AOX1-α factor-ACC-AOX1 TerminatorComposite3271
BBa_K3711048AOX1-α factor-CUS-AOX1 TerminatorComposite2698
BBa_K3711049AOX1-α factor-curA-AOX1 TerminatorComposite2527
BBa_K3711050AOX1-α factor-PepACS-AOX1 TerminatorComposite1639
BBa_K3711051AOX1-α factor-DsbC-AOX1 TerminatorComposite2374
BBa_K3711052Pynr071c-α factor-FMO dimer-AOX1 TerminatorComposite4491
BBa_K3711053Pynr071c-α factor-ROX1-AOX1 TerminatorComposite2973
BBa_K3711054Panb1-α factor-FMO-AOX1 TerminatorComposite2328
BBa_K3711055Pynr071c-α factor-Laccase-AOX1 TerminatorComposite3324
BBa_K3711056Panb1-α factor-crtE-AOX1 TerminatorComposite1860
BBa_K3711057Panb1-α factor-crtB-AOX1 TerminatorComposite1881
BBa_K3711058Panb1-α factor-crtI-AOX1 TerminatorComposite2430
BBa_K3711060Pynr071c-α factor-LOX2-AOX1 TerminatorComposite4431
BBa_K3711061Panb1-α factor-4CL-AOX1 TerminatorComposite2637
BBa_K3711062Panb1-α factor-ACC-AOX1 TerminatorComposite2733
BBa_K3711063Panb1-α factor-CUS-AOX1 TerminatorComposite2160
BBa_K3711064Pynr071c-α factor-curA-AOX1 TerminatorComposite2778
BBa_K3711065Panb1-α factor-PepACS-AOX1 TerminatorComposite1101
BBa_K3711066Pynr071c-α factor-DsbC-AOX1 TerminatorComposite2625
BBa_K3711067Pynr071c-ROX1-AOX1 TerminatorComposite2700
BBa_K3711068Panb1-FMO-AOX1 TerminatorComposite2055
BBa_K3711069Pynr071c-Laccase-AOX1 TerminatorComposite3051
BBa_K3711070Panb1-crtE-AOX1 TerminatorComposite1587
BBa_K3711071Panb1-crtB-AOX1 TerminatorComposite1608
BBa_K3711072Panb1-crtI-AOX1 TerminatorComposite2157
BBa_K3711073Pynr071c-LOX2-AOX1 TerminatorComposite4158
BBa_K3711074Panb1-4CL-AOX1 TerminatorComposite2364
BBa_K3711075Panb1-ACC-AOX1 TerminatorComposite2460
BBa_K3711076Panb1-CUS-AOX1 TerminatorComposite1887
BBa_K3711077Pynr071c-curA-AOX1 TerminatorComposite2505
BBa_K3711078Panb1-PepACS-AOX1 TerminatorComposite828
BBa_K3711079Pynr071c-DsbC-AOX1 TerminatorComposite2352
BBa_K3711080AOX1-α factor-Lycopene-AOX1 TerminatorComposite7801
BBa_K3711081AOX1-α factor-Curcumin-AOX1 TerminatorComposite9160
BBa_K3711082Panb1-α factor-Curcumin-AOX1 TerminatorComposite7546
BBa_K3711083Panb1-α factor-Lycopene-AOX1 TerminatorComposite6187
BBa_K3711084Panb1-Curcumin-AOX1 TerminatorComposite6727
BBa_K3711085Panb1-Lycopene-AOX1 TerminatorComposite5368
BBa_K3711086The synthesis and decolorization system of indigoComposite8368
BBa_K3711087The synthesis and decolorization system of indigoComposite7822
BBa_K3711088The synthesis and decolorization system of indigoComposite14948
BBa_K3711089The synthesis and decolorization system of lycopene without signal peptideComposite12242
BBa_K3711090The synthesis and decolorization system of lycopene without signal peptideComposite13040
BBa_K3711091The synthesis and decolorization system of curcumin without signal peptideComposite11948
BBa_K3711092The perm and straightening systemComposite6442
BBa_K3711093The perm and straightening system without signal peptideComposite5896

How ingenious! It seems that constructing composite parts and full pathway takes engineering wisdom.

CONTACT US: iGEMHUSTChina@163.com

Huazhong University of Sci. & Tech., Wuhan, China

1037# Luoyu Rd, Wuhan, P.R.China 430074

Copyright © HUST-China iGEM 2021