Difference between revisions of "Team:Shanghai Metro HS"

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<h1> Welcome to iGEM 2021! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h3>Before you start</h3>
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<p> Please read the following pages:</p>
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<li> <a href="https://2021.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2021.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2021.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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<h3> Styling your wiki </h3>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<h3> Uploading pictures and files </h3>
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<p> You must upload any pictures and files to the iGEM 2021 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<h3> Wiki template information </h3>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2021.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<h3> Editing your wiki </h3>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<a href="https://2021.igem.org/wiki/index.php?title=Team:Shanghai_Metro_HS&action=edit">
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EDIT PAGE
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<h3>Tips</h3>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2021.igem.org/Calendar">iGEM 2021 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h3>Inspiration</h3>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<ul>
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<li> <a href="https://2019.igem.org/Team:Vilnius-Lithuania"> 2019 Vilnius Lithuania</a> </li>
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<li> <a href="https://2019.igem.org/Team:NUS_Singapore">2019 NUS Singapore</a> </li>
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<li> <a href="https://2019.igem.org/Team:Lambert_GA">2019 Lambert GA</a> </li>
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<li> <a href="https://2020.igem.org/Team:Waterloo"> 2020 Waterloo</a></li>
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<li> <a href="https://2020.igem.org/Team:TUDelft">2020 TUDelft</a></li>
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<li> <a href="https://2020.igem.org/Team:XMU-China"> 2020 XMU China </a></li>
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<li> <a href="https://2020.igem.org/Team:TAS_Taipei"> 2020 TAS Taipei </a></li>
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</ul>
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    <title>Shanghai_Metro_HS</title>
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        href="https://2021.igem.org/wiki/index.php?title=Template:Shanghai_Metro_HS/Main_CSS&action=raw&ctype=text/css" />
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        href="https://2021.igem.org/wiki/index.php?title=Template:Shanghai_United_HS/Swiper_CSS&action=raw&ctype=text/css">
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    <nav class="head-nav clearfix">
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        <div class="top-nav-bar">
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            <ul class="clearfix">
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                <li class="active">
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                    <a href="#">Home</a>
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                </li>
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                <li>
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                    <a href="">Project</a>
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                    <div class="sub-nav">
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                        <ul>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Description"
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                                    class="sub-nav-74">Description</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Experiments"
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                                    class="sub-nav-74">Experiments</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Results"
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                                    class="sub-nav-74">Results</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/ProofOfConcept"
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                                    class="sub-nav-52">Proof Of
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                                    Concept</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Notebook"
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                                    class="sub-nav-52">Notebook</a>
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                            </li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Safety">Safety</a></li>
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                        </ul>
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                    </div>
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                </li>
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                    <a href="">Parts</a>
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                    <div class="sub-nav">
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                        <ul>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Collection"
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                                    class="sub-nav-74">Parts
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                                    Collection</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Engineering"
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                                    class="sub-nav-74">Engineering</a></li>
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                        </ul>
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                </li>
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                <li>
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                    <a href="">Human Practices</a>
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                    <div class="sub-nav">
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                        <ul>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Integrated"
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                                    class="sub-nav-74">Integrated
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                                    Human Practice</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Communication"
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                                    class="sub-nav-74">Communication</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Fundraising"
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                                    class="sub-nav-74">Fundraising</a></li>
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                        </ul>
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                    </div>
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                </li>
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                <li>
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                    <a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Implementation">Implementation</a>
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                </li>
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                <li>
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                    <a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Entrepreneurship">Entrepreneurship</a>
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                </li>
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                <li>
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                    <a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Model">Model</a>
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                </li>
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                <li>
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                    <a href="">Team</a>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Members" class="sub-nav-74">Team
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                                    Members</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Attributions"
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                                    class="sub-nav-74">Attributions</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_HS/Collaborations"
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                                    class="sub-nav-74">Collaborations</a></li>
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        <div class="team-tips">
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            <p class="team-tips-title">ABSTRACT</p>
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                <p class="team-tips-content">Our project aims to create a gene-edited E.coli bacteria as a silage
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                    additive that can help improve the digestion of ruminants on silage and further improve the quality
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                    of the livestock. In the selection of the desired gene, PKC-001 from Pseudomonas aeruginosa was
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                    reported to secrete the cellulase which maintains well activity under both acid and base conditions.
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                    Therefore, we optimized its codon and further constructed the plasmid to combine the optimized
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                    gene-PKC-OP, which was transformed into the E. coli for expressing itself. Even though we
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                    encountered many difficulties in lab work, we finally obtained our engineered E. coli which could
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                    express the desired cellulase. We hope this silage additive could contribute the stockbreeding
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                    development and improve life quality in the future.</p>
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            </div>
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        </div>
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        <video width="100%" height="690" controls class="home-video">
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            <source src="https://static.igem.org/mediawiki/2021/4/45/T--Shanghai_Metro_HS--video.mp4" type="video/mp4">
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    <footer class="footer">
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        <section class="footer-wrap">
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            <div class="footer-contact">CONTACT INFO</div>
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            <p class="margin-bottom-20">Email: igemteamceres@gmail.com</p>
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            <p><i style="color: #2d2d2d;">WeChat Official Account: iGEM Ceres</i></p>
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        </section>
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    </footer>
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</body>
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Revision as of 15:21, 28 September 2021

Shanghai_Metro_HS

ABSTRACT

Our project aims to create a gene-edited E.coli bacteria as a silage additive that can help improve the digestion of ruminants on silage and further improve the quality of the livestock. In the selection of the desired gene, PKC-001 from Pseudomonas aeruginosa was reported to secrete the cellulase which maintains well activity under both acid and base conditions. Therefore, we optimized its codon and further constructed the plasmid to combine the optimized gene-PKC-OP, which was transformed into the E. coli for expressing itself. Even though we encountered many difficulties in lab work, we finally obtained our engineered E. coli which could express the desired cellulase. We hope this silage additive could contribute the stockbreeding development and improve life quality in the future.

CONTACT INFO

Email: igemteamceres@gmail.com

WeChat Official Account: iGEM Ceres