Difference between revisions of "Team:Shanghai HS United/Results"

(Prototype team page)
 
Line 1: Line 1:
−
{{IGEM_TopBar}}
 
−
{{Shanghai_HS_United}}
 
 
<html>
 
<html>
  
−
<div class="column full_size">
+
<link rel="stylesheet" type="text/css"
−
<h1>Results</h1>
+
      href="https://2021.igem.org/wiki/index.php?title=Template:Shanghai_HS_United/StyleCSS&action=raw&ctype=text/css"/>
−
<p>You can describe the results of your project and your future plans here. </p>
+
−
</div>
+
  
 +
<div class="flavone-lab-content">
 +
    <!--navbar-->
 +
    <div class="navbar">
 +
        <a class="logo" href="https://2021.igem.org/Team:Shanghai_HS_United">
 +
            <img src="https://static.igem.org/mediawiki/2021/c/c1/T--Shanghai_HS_United--logo.png" alt="">
 +
        </a>
  
−
<div class="column third_size" >
+
        <!--nav-->
 +
        <ul class="list">
 +
            <!--Team-->
 +
            <li class="item">
 +
                <a href="https://2021.igem.org/Team:Shanghai_HS_United/Team">Team</a>
 +
                <div class="child-nav">
 +
                    <ul class="child-list">
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Team">Team Members</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Attributions">Attributions</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Collaborations">Collaboration</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Partnership">Partnership</a>
 +
                        </li>
 +
                    </ul>
 +
                </div>
 +
            </li>
  
−
<h3>What should this page contain?</h3>
+
            <!--Modeling-->
−
<ul>
+
            <li class="item">
−
<li> Clearly and objectively describe the results of your work.</li>
+
                <a href="https://2021.igem.org/Team:Shanghai_HS_United/Model">Modeling</a>
−
<li> Future plans for the project. </li>
+
            </li>
−
<li> Considerations for replicating the experiments. </li>
+
−
</ul>
+
−
</div>
+
  
 +
            <!--Entrepreneurship-->
 +
            <li class="item">
 +
                <a href="https://2021.igem.org/Team:Shanghai_HS_United/Entrepreneurship">Entrepreneurship</a>
 +
            </li>
  
 +
            <!--Implementation-->
 +
            <li class="item">
 +
                <a href="https://2021.igem.org/Team:Shanghai_HS_United/Implementation">Implementation</a>
 +
            </li>
  
 +
            <!--Human Practice-->
 +
            <li class="item">
 +
                <a href="https://2021.igem.org/Team:Shanghai_HS_United/Human_Practices">Human Practice</a>
 +
                <div class="child-nav">
 +
                    <ul class="child-list">
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Human_Practices">Integrated Human
 +
                                Practice</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Communication">Communication</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Fundraising">Fundraising</a>
 +
                        </li>
 +
                    </ul>
 +
                </div>
 +
            </li>
  
−
<div class="column two_thirds_size" >
+
            <!--Parts-->
−
<h3>Describe what your results mean </h3>
+
            <li class="item">
−
<ul>
+
                <a href="https://2021.igem.org/Team:Shanghai_HS_United/PartsCollection">Parts</a>
−
<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
+
                <div class="child-nav">
−
<li> Show data, but remember <b>all measurement and characterization data must also be on the Part's Main Page on the <a href="http://parts.igem.org/Main_Page">Registry</a>.</b> Otherwise these data will not be in consideration for any medals or part awards! </li>
+
                    <ul class="child-list">
−
<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
+
                        <li class="child-item">
−
</ul>
+
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Parts_Collection">Parts
−
</div>
+
                                Collection</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Engineering">Engineering</a>
 +
                        </li>
 +
                    </ul>
 +
                </div>
 +
            </li>
  
 +
            <!--Project-->
 +
            <li class="item active">
 +
                <a href="https://2021.igem.org/Team:Shanghai_HS_United/Description">Project</a>
 +
                <div class="child-nav">
 +
                    <ul class="child-list">
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Description">Description</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Experiments">Experiments</a>
 +
                        </li>
 +
                        <li class="child-item active">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Results">Result</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/ProofOfConcept">Proof of Concept</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Notebook">Notebook</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Safety">Safety</a>
 +
                        </li>
 +
                    </ul>
 +
                </div>
 +
            </li>
  
−
<div class="clear extra_space"></div>
+
            <!--Home-->
 +
            <li class="item">
 +
                <a href="https://2021.igem.org/Team:Shanghai_HS_United">Home</a>
 +
            </li>
 +
        </ul>
 +
    </div>
  
 +
    <!--图片-->
 +
    <div class="page-header">
 +
        <img src="https://static.igem.org/mediawiki/2021/9/97/T--Shanghai_HS_United--bg_1.png" alt="">
 +
        <h1>Results</h1>
 +
    </div>
  
 +
    <!--内容-->
 +
    <div class="content-small">
 +
        <section class="article p-t-72 p-b-54">
 +
            <section class="en-indent-all">
 +
                <h1 class="title">Enzyme Activity Test</h1>
 +
                <p>After we obtained the purified protein, namely the FLR enzyme, we conducted enzyme activity tests by
 +
                    using 4 kinds of flavonoids samples: apigenin, chrysin, luteolin, diosmetin with the initial
 +
                    concentration 10mg/L and the concentration of FLR enzyme was 1mM/L. Each sample was guaranteed three
 +
                    replicates of the enzyme activity test in order to gain more data to ensure the credibility of the
 +
                    result of our experiment.</p>
  
−
<div class="column two_thirds_size" >
+
                <div class="img-container">
−
<h3> Project Achievements </h3>
+
                    <img src="https://static.igem.org/mediawiki/2021/8/81/T--Shanghai_HS_United--img_result_1.jpg" alt="" style="width: 80%">
 +
                    <span class="figure">Figure 1. Bar graph of the enzyme activity test results</span>
 +
                </div>
  
−
<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
+
                <p>Figure 1 shows that the concentration of these 4 flavonoids remarkably decreases after 2 hours which were
 +
                    degraded by the enzyme, even there were only 0.3-5% left after 6 hours. All these results indicate that
 +
                    our enzyme has high activity and is capable of degrading multiple flavonoids with a certain
 +
                    universality.</p>
 +
            </section>
  
−
<ul>
+
            <section class="en-indent-all">
−
<li>A list of linked bullet points of the successful results during your project</li>
+
                <h1 class="title">Bacteria Activity Test</h1>
−
<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
+
                <p>In order to analyze the performance of our engineered E. coli, we also conducted the activity test by
−
</ul>
+
                    using the same 4 kinds of flavonoids samples: apigenin, chrysin, luteolin, diosmetin with the initial
 +
                    concentration 10mg/L and the OD600 was 1.0. Each sample was guaranteed three replicates of the activity
 +
                    test in order to gain more data to ensure the credibility of the result of our experiment.</p>
  
−
</div>
+
                <div class="img-container">
 +
                    <img src="https://static.igem.org/mediawiki/2021/5/56/T--Shanghai_HS_United--img_result_2.jpg" alt="" style="width: 80%">
 +
                    <span class="figure">Figure 2. Bar graph of the bacteria activity test results</span>
 +
                </div>
  
 +
                <p>In figure 2, you could easily tell that all these flavonoids were almost degraded after 2 hours with only
 +
                    1-7% left, which indicates the strong ability of our engineered E. coli to degrade flavonoids as we
 +
                    expected.</p>
  
 +
                <p>Above all, we could come to the conclusion that the FLR enzyme, as well as the engineered E. coli, is
 +
                    capable of degrading various flavonoids with a certain universality and practicability. In comparison,
 +
                    the degradability of FLR enzyme to these flavonoids could be ranked from greatest to least as apigenin >
 +
                    chrysin > luteolin > diosmetin.</p>
 +
            </section>
  
−
<div class="column third_size" >
+
            <section class="en-indent-all">
−
<div class="highlight decoration_A_full">
+
                <h1 class="title">Future Plan</h1>
−
<h3>Inspiration</h3>
+
−
<p>See how other teams presented their results.</p>
+
−
<ul>
+
−
<li><a href="https://2019.igem.org/Team:Newcastle/Results">2019 Newcastle</a></li>
+
−
<li><a href="https://2019.igem.org/Team:Munich/Results">2019 Munich </a></li>
+
−
<li><a href="https://2019.igem.org/Team:Tec-Chihuahua/Results">2019 Tec Chihuahua</a></li>
+
−
<li><a href="https://2020.igem.org/Team:Aalto-Helsinki/Results">2020 Aalto Helsinki</a></li>
+
−
<li><a href="https://2020.igem.org/Team:GreatBay_SCIE/Results">2020 GreatBay SCIE</a></li>
+
−
<li><a href="https://2020.igem.org/Team:Queens_Canada/Results">2020 Queens Canada</a></li>
+
  
−
</ul>
+
                <p>Since the FLR enzyme showed a great ability to decompose flavonoids, we are looking forward to testing
 +
                    how the human body would consume with and without the FLR enzyme. What’s more, if the further experiment
 +
                    succeeds, we will probably try to add the FLR engineered bacteria into yogurt. Yogurt contains a large
 +
                    number of lactic acid bacteria, which themselves belong to acidic substances. After use, they can
 +
                    promote gastrointestinal peristalsis and food digestion. Yogurt helps in digesting food and FLR helps in
 +
                    digesting flavonoids. By adding it, we could make a health-care yogurt which is great for human health
 +
                    improvement.</p>
 +
            </section>
 +
        </section>
 +
    </div>
 +
 
 +
    <!--Footer-->
 +
    <div class="footer">
 +
        <div class="content">
 +
            <hr>
 +
            <span>Email: xiaowenxuan0526@gmail.com; caroline.niujl@gmail.com</span>
 +
            <span>Wechat Official Account: FlavoneLabRouse</span>
 +
        </div>
 +
    </div>
 
</div>
 
</div>
−
</div>
 
−
 
−
 
−
 
−
 
−
 
 
</html>
 
</html>

Revision as of 06:48, 17 September 2021

Results

Enzyme Activity Test

After we obtained the purified protein, namely the FLR enzyme, we conducted enzyme activity tests by using 4 kinds of flavonoids samples: apigenin, chrysin, luteolin, diosmetin with the initial concentration 10mg/L and the concentration of FLR enzyme was 1mM/L. Each sample was guaranteed three replicates of the enzyme activity test in order to gain more data to ensure the credibility of the result of our experiment.

Figure 1. Bar graph of the enzyme activity test results

Figure 1 shows that the concentration of these 4 flavonoids remarkably decreases after 2 hours which were degraded by the enzyme, even there were only 0.3-5% left after 6 hours. All these results indicate that our enzyme has high activity and is capable of degrading multiple flavonoids with a certain universality.

Bacteria Activity Test

In order to analyze the performance of our engineered E. coli, we also conducted the activity test by using the same 4 kinds of flavonoids samples: apigenin, chrysin, luteolin, diosmetin with the initial concentration 10mg/L and the OD600 was 1.0. Each sample was guaranteed three replicates of the activity test in order to gain more data to ensure the credibility of the result of our experiment.

Figure 2. Bar graph of the bacteria activity test results

In figure 2, you could easily tell that all these flavonoids were almost degraded after 2 hours with only 1-7% left, which indicates the strong ability of our engineered E. coli to degrade flavonoids as we expected.

Above all, we could come to the conclusion that the FLR enzyme, as well as the engineered E. coli, is capable of degrading various flavonoids with a certain universality and practicability. In comparison, the degradability of FLR enzyme to these flavonoids could be ranked from greatest to least as apigenin > chrysin > luteolin > diosmetin.

Future Plan

Since the FLR enzyme showed a great ability to decompose flavonoids, we are looking forward to testing how the human body would consume with and without the FLR enzyme. What’s more, if the further experiment succeeds, we will probably try to add the FLR engineered bacteria into yogurt. Yogurt contains a large number of lactic acid bacteria, which themselves belong to acidic substances. After use, they can promote gastrointestinal peristalsis and food digestion. Yogurt helps in digesting food and FLR helps in digesting flavonoids. By adding it, we could make a health-care yogurt which is great for human health improvement.

Email: xiaowenxuan0526@gmail.com; caroline.niujl@gmail.com Wechat Official Account: FlavoneLabRouse